GB/T 36775-2018 English PDF

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GB/T 36775-2018: Detection and identification of Pseudocercospora angolensis Crous & U. Braun
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36775-2018	279	Add to Cart	3 days	Detection and identification of Pseudocercospora angolensis Crous & U. Braun	Valid

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Basic data

Standard ID: GB/T 36775-2018 (GB/T36775-2018)
Description (Translated English): Detection and identification of Pseudocercospora angolensis Crous & U. Braun
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,149
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Regulation (derived from): National Standard Announcement No. 11 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36775-2018: Detection and identification of Pseudocercospora angolensis Crous & U. Braun

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Pseudocercospora angolensis Crous ICS 65.020.01 B16 National Standards of People's Republic of China Method for quarantine and identification of citrus spotted bacteria Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Feng Lixia, Hu Xuenan, Zuo Ranling, Wu Mutao, Wang Weifang, Zhao Lirong, Yu Yu. Method for quarantine and identification of citrus spotted bacteria

1 Scope

This standard specifies the quarantine and identification methods for citrus spotted bacteria in plant quarantine. This standard applies to the quarantine and identification of citrus spotted bacteria in citrus seedlings and fruits.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 1538.1 Guidelines for the preparation of medium - Part 1

3 Principle of the method

See Appendix A for the classification, distribution and host information of citrus spotted bacteria. This standard uses the pathogen to host the symptoms, morphological characteristics, The culture characteristics were used as the basis for the main quarantine and identification, and the pathogenicity test was used as the basis for the auxiliary quarantine identification of citrus spotted bacteria.

4 equipment

4.1 Instrumentation Stereo microscope, biological microscope, ultra-clean workbench, autoclave, electronic balance (sensitivity 0.01g), biological incubator. 4.2 Laboratory equipment Labels, qualitative filter paper, sample bags, storage bags, hand-held magnifiers, tweezers, scissors, scalpels, alcohol lamps, slides, coverslips, inoculation needles, Petri dishes, beakers, measuring cylinders, flasks, glass rods, micropipettes (with a range of 2.5 μL to 1000 μL) and pH meter.

5 reagents and media

5.1 Reagents Anhydrous ethanol, 0.5% sodium hypochlorite (NaOCl) solution, calcium carbonate (CaCO3), sterile double distilled water. 5.2 medium Potato Dextrose Agar Medium (PDA). Weigh.200 g of potato, 15 g of glucose, and 15 g of agar. Potato peeled and cut into small After the block, add water and boil for 20min~30min. After filtering the gauze, add 15g agar, dissolve and add 15g of glucose, and make up the deionized water. 1000 mL, the pH of the medium was adjusted to 5.6, and sterilized at 121 ° C for 20 min. Commercialized PDA powder can also be used according to the instructions for use. Formulated. V8 medium (V8A). 100mL V8 vegetable juice, calcium carbonate 0.2g, agar powder 150g, deionized water to make up 1000mL, 121 ° C ......

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