GB/T 35798-2018 English PDF

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GB/T 35798-2018: Determination of coumarin and its derivatives in cosmetics -- High performance liquid chromatography
Status: Valid

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Basic data

Standard ID: GB/T 35798-2018 (GB/T35798-2018)
Description (Translated English): Determination of coumarin and its derivatives in cosmetics -- High performance liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: Y42
Classification of International Standard: 71.100.70
Word Count Estimation: 14,159
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35798-2018: Determination of coumarin and its derivatives in cosmetics -- High performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of coumarin and its derivatives in cosmetics - High performance liquid chromatography ICS 71.100.70 Y42 National Standards of People's Republic of China Determination of coumarin and its derivatives in cosmetics High performance liquid chromatography 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the China Light Industry Federation. This standard by the National Spice cosmetics Standardization Technical Committee (SAC/TC257) centralized. This standard was drafted. China Academy of Inspection and Quarantine, Shanghai Daily Chemical Industry Institute. The main drafters of this standard. Meng Xian double, Ma Qiang, Wang Hongwei, Lu Qing, Li Junfang, Li Yahui, Wang Feng, Shen Min.

Introduction

Some of the standard substances (dicoumarol, 7-methoxycoumarin, dihydrocoumarin, acenocoumarol, 7-methyl coumarin, 7-B Oxy-4-methyl-coumarin and cyclo-coumarin) are banned substances in China's "Cosmetic Safety Technical Specification". Prohibited substances are those that can not be added to cosmetics as a raw material for the production of cosmetics. "Cosmetics Safety Specifications" rules Determine. If technically unavoidable banned substances as impurities into the cosmetics, safety risk assessment should be carried out to ensure that the normal, reasonable and may Under the foreseeable conditions of use, no harm to human health may occur. At present, China has not provided for the limited value of these substances, the development of this standard, only for the detection of these substances in cosmetics to provide testing methods. Determination of coumarin and its derivatives in cosmetics High performance liquid chromatography

1 Scope

This standard specifies the method for the determination of coumarin and dicoumarin and other 7 coumarin derivatives in cosmetics by high performance liquid chromatography. This standard applies to agents, creams, shampoos, loose powder and lipstick cosmetics coumarin and two coumarin and other coumarin derivatives The determination. Detection limits and quantitative limits of this standard. coumarin detection limit of 4mg/kg, the limit of quantification of 8mg/kg; the detection limit of dihydrocoumarin Is 10 mg/kg and the limit of quantification is 20 mg/kg; 7-methoxycoumarin, 7-methylcoumarin, 7-ethoxy-4-methylcoumarin, The detection limits of dicoumarol and acenocoumarol were 5 mg/kg and the limit of quantification was 10 mg/kg.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 principle

The sample was extracted by solvent, centrifuged, and filtered through a microporous filter. The sample was confirmed by high performance liquid chromatography, external standard method, and liquid chromatography-mass spectrometry.

4 Reagents and materials

Unless otherwise indicated, the reagents used are of analytical grade, water is a water GB/T 6682 regulations. 4.1 Acetonitrile. Chromatographic purity. 4.2 methanol. pure chromatography. 4.3 Dichloromethane. 4.4 Tetrahydrofuran. 4.5 Ammonium acetate. 4.6 glacial acetic acid. 4.7 Sodium hydroxide. 4.8 0.05mol/L ammonium acetate solution. Weigh 1.925g of ammonium acetate (4.5), dissolved in water and transferred to 500mL volumetric flask, with water Allow to scale, shake, spare. 4.9 0.1mol/L sodium hydroxide solution. Weigh 0.400g sodium hydroxide (4.7), dissolved in water and transferred to a 100mL volumetric flask, constant volume To the scale. 4.10 0.1mol/L sodium hydroxide - acetonitrile mixed solution (19) (volume volume). Measure 90mL acetonitrile (4.1) to 100mL capacity Bottle, add 0.1mol/L sodium hydroxide solution (4.9) to the mark, mix well. 4.11 coumarin, dihydrocoumarin, 7-methoxycoumarin, 7-methylcoumarin, 7-ethoxy-4-methylcoumarin, cyclocoumarin, The Chinese name, English name, CAS number, molecular formula, structural formula and relative molecular mass of allotroxen and coumarin are given in Appendix A ......

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