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GB/T 34777-2017 English PDF

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US$119.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 34777-2017: Detection of residual DNA in biological product which expressed from Chinese hamster ovary (CHO) cell -- Fluorescent quantitative polymerase chain reaction assay
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34777-2017	119	Add to Cart	3 days	Detection of residual DNA in biological product which expressed from Chinese hamster ovary (CHO) cell -- Fluorescent quantitative polymerase chain reaction assay	Valid

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Basic data

Standard ID: GB/T 34777-2017 (GB/T34777-2017)
Description (Translated English): Detection of residual DNA in biological product which expressed from Chinese hamster ovary (CHO) cell -- Fluorescent quantitative polymerase chain reaction assay
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Classification of International Standard: 07.080
Word Count Estimation: 6,668
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Regulation (derived from): National Standard Announcement 2017 No. 29
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34777-2017: Detection of residual DNA in biological product which expressed from Chinese hamster ovary (CHO) cell -- Fluorescent quantitative polymerase chain reaction assay

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of residual DNA in biological product which expressed from Chinese hamster ovary (CHO) cell-Fluorescent quantitative polymerase chain reaction assay ICS 07.080 A40 National Standards of People's Republic of China Chinese Hamster Ovary (CHO) Cells Express Products Residual DNA Detection Fluorescent Quantitative PCR Chinesehamsterovary (CHO) cel- Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized. This standard drafting unit. Cinda biological pharmaceutical (Suzhou) Co., Ltd., China Institute of Tested Technology Institute of Biology. The main drafters of this standard.Sun Zuoyu, Zhou Lihua, Li Xuefeng, Huang Xiaogang, Yuan Zhijun, Guo Yanhong. Chinese Hamster Ovary (CHO) Cells Express Products Residual DNA Detection Fluorescent Quantitative PCR

1 Scope

This standard specifies the detection of residual host DNA by fluorescence quantitative PCR method. This standard applies to the CHO (Chinese Hamster Ovary cells) host cell line expressed in the biological products of the detection of the host DNA.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Ct value cyclethreshold The number of cycles experienced when the fluorescent signal in each reaction tube reached a set threshold.

4 Abbreviations

The following abbreviations apply to this document. CHO. Chinese Hamster Ovary

5 principle

In the eukaryotic cell genome with high copy number of 16SrRNA gene, designed to specifically amplify the gene PCR Forward primer, ForwardPrimer (5'-CCAGGCATTGGTGGCAC-3 ') and reverse primer, ReversePrimer (5'-AGACAGGGTTTCTCTGT-3 '). The SYBR fluorescence quantitative PCR (FQ-PCR) method was used to acquire the Ct value of the PCR amplification curve drawn by the fluorescence and was used in the amplification system The original purpose of the template is inversely proportional to the logarithm of the initial template DNA logarithm of the vertical axis, Ct value of the abscissa, making a standard curve. According to the standard curve equation to calculate the sample of DNA residues, so as to quantitatively detect the content of residual DNA samples purpose.

6 equipment

6.1 Fluorescence quantitative PCR instrument containing SYBR fluorescent dye detection channel. 6.2 UV - visible spectrophotometer. Wavelength range .200nm ~ 760nm. 6.3 Microcentrifuge. Speed range..2000r/min ~ 23000r/min. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 34777-2017_English be delivered?

Answer: Upon your order, we will start to translate GB/T 34777-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 34777-2017_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 34777-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.