GB/T 34776-2017 English PDF

US$179.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 34776-2017: T4 DNA ligase -- Detection methods of activity and impurities of enzyme
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34776-2017	179	Add to Cart	3 days	T4 DNA ligase -- Detection methods of activity and impurities of enzyme	Valid

Similar standards

GB/T 38505   GB/T 38132   GB/T 36081   GB/T 43708   GB/T 34777   

Basic data

Standard ID: GB/T 34776-2017 (GB/T34776-2017)
Description (Translated English): T4 DNA ligase -- Detection methods of activity and impurities of enzyme
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Classification of International Standard: 07.080
Word Count Estimation: 9,964
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Regulation (derived from): National Standard Announcement 2017 No. 29
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34776-2017: T4 DNA ligase -- Detection methods of activity and impurities of enzyme

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
T4 DNA ligase-Detection methods of activity and impurities of enzyme ICS 07.080 A40 National Standards of People's Republic of China T4 DNA ligase activity and impurity detection methods Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard by the National Tool Enzyme Working Group (SAC/SWG11) centralized. This standard drafting unit. China Institute of Testing Technology, Shenzhen Huaneng Kang Gene Technology Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd. The main drafters of this standard.Zhou Lihua, Li Huaping, Sheng Tong, Sun Dengfeng, Tan Huibiao, Huangfa Can, Jin Hong, Ma Li Xia, Ye Deping, Li Hua, Wang Zhi, Shen Xingzhong, Tan peace. T4 DNA ligase activity and impurity detection methods

1 Scope

This standard specifies the T4DNA ligase activity, impurity detection methods. This standard applies to research as a molecular biology T4DNA ligase.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods YY/T 0087 electrophoresis device YY/T 0657 medical centrifuge JJG646 pipette test procedures

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 DNA ligase DNAligase A gapped enzyme that blocks the DNA strand, catalyzing the 3'-hydroxylation between adjacent nucleotide molecules with the aid of the energy provided by ATP or NAD hydrolysis The base and the 5'-phosphate group form a phosphodiester bond. Note. DNA ligase-catalyzed substrates can be either DNA or RNA. Depending on the type of enzyme, cofactors that produce high energy intermediates in the reaction may be ATP or NAD. 3.2 T4 DNA ligase T4 DNAligase A T4 phage gene 30 encoding product, with ATP as a cofactor, catalyzes the double-stranded DNA or RNA 5'-phosphate end The terminal and 3'-hydroxyl ends form phosphodiester bonds. Note. His-Tag, with a molecular mass of approximately 70 ku, is derived from the E. coli strain transformed with T4 DNA ligase expression vector. The main role is catalysis Linkage between DNA ends repairs single-stranded nicks in double-stranded nucleic acids. 3.3 Unit of activity In 20μL 1 × T4 DNA ligase reaction buffer, reaction conditions at 16 ℃, 30min can make 50% Hind Ⅲ eliminate The amount of enzyme required to ligate the [lambda] DNA fragment [5 'end of 0.12 [mu] mol/L (300 [mu] g/mL] is 1 unit of activity.

4 Abbreviations

The following abbreviations apply to this document. PCR polymerase chain reaction (polymerasechainreaction). SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sodium dodecylsulfate-polyacrylamide gel ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 34776-2017_English be delivered?

Answer: Upon your order, we will start to translate GB/T 34776-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 34776-2017_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 34776-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.