GB/T 33681-2017 English PDFGB/T 33681: Historical versions
Basic dataStandard ID: GB/T 33681-2017 (GB/T33681-2017)Description (Translated English): (Pretreatment of high throughput gene sequencing samples -- Part 1: Pretreatment of animal tissue samples) Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: A40 Date of Issue: 2017-05-12 Date of Implementation: 2017-12-01 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China GB/T 33681.1-2017: Methods to prepare samples for high-throughput gene sequencing -- Part 1: Preparing samples of animal tissues---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Methods to prepare samples for high-throughput gene sequencing - Part 1. Preparing samples of animal tissues ICS 07.080 A40 National Standards of People's Republic of China Pretreatment of high throughput gene sequencing samples Part 1. Animal tissue sample pretreatment Part 1. Preparingamplesofanimaltissues 2017-05-12 released 2017-12-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released Directory Preface I 1 Scope 1 2 normative reference document 1 3 Terms and definitions 1 4 acronym 2 5 General 2 6 laboratory requirements 3 Main equipment and equipment 3 8 Test Step 3 Quality assessment 4 Appendix A (informative) Extraction of nucleic acids 5 Appendix B (informative) Quality evaluation 6 Appendix C (normative appendix) Laboratory requirements 7 Appendix D (informative) Fragmentation 11 Appendix E (informative) Single molecule amplification 13 Reference 14 ForewordGB/T 33681 "high-throughput gene sequencing sample pretreatment method" is divided into three parts. Part 1 Animal tissue sample pretreatment; Part 2 Plant Tissue Sample Pretreatment; Part 3 Microorganism Tissue Sample Pretreatment. This part is part 1 of GB/T 33681. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part of the National Biochemical Testing Standardization Technical Committee (SAC/TC387) proposed and centralized. This part of the drafting unit. Shenzhen Hua Kang Gene Technology Co., Ltd., Shenzhen Hua Kang high-throughput biotechnology research institute, Guangzhou Kang Xin Ruike Health Technology Co., Ltd., Wuhan Kang Xinrui Gene Health Technology Co., Ltd., China Testing Technology Research Institute, Shanghai Jiaotong University College Affiliated Ruijin Hospital, National Human Genome Research Center of the South, Zhongshan University Cancer Center. The main drafters of this part. Sheng Sitong, Tan Hui Biao, Li Hua, Zeng Tao, Yang Jiebin, Zhou Lihua, Zhang Jun, Wang Shengyue, Chen Gong. Pretreatment of high throughput gene sequencing samples Part 1. Animal tissue sample pretreatment1 ScopeThis part of GB/T 33681 specifies the technique for pre-processing samples of animal tissue samples to be tested in high-throughput gene sequencing techniques Claim. This part is suitable for the pretreatment of samples of animal tissues to be tested during high-throughput gene sequencing. This part applies to blood, epidermal swab samples, fresh animal tissue samples (muscles, organs, hair, bones), formaldehyde fixed animal tissue Sample, paraffin embedded animal tissue samples.2 normative reference documentsThe following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 26798 single - beam UV - Vis spectrophotometer Dual - beam UV - Vis spectrophotometer GB/T 26813 GB/T 27025 General requirements for testing and calibration laboratory capabilities Technical specification for high - throughput gene sequencing GB/T JJG646 pipette YY0569 Ⅱ level biological safety cabinet YY/T 0657 Medical centrifuge Medical institutions, clinical gene amplification management approach ASTME1342 Preserves bacteria, fungi, protozoa, viruses, genetic elements and animals by freezing, freeze-drying and cryopreservation Standards and regulations for plant and plant tissue (Standardpracticeforpreservationbyfreezing, freeze-drying, andlow temperaturemaintenanceofbacteria, fungi, protista, viruses, geneticelements, andanimalandplant tissues3 terms and definitionsGB/T 30989 Definitions and the following terms and definitions apply to this document. 3.1 Animal tissue sample animaltissuesample An animal tissue material that provides DNA or RNA for constructing a sequencing library. 3.2 Connector adapter A fragment of a synthetic length that is capable of matching (concatenating) a blunt end or a cohesive end. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 33681-2017_English be delivered?Answer: Upon your order, we will start to translate GB/T 33681-2017_English as soon as possible, and keep you informed of the progress. 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Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.Question 5: Should I purchase the latest version GB/T 33681-2017?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 33681-2017 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |
