GB/T 33682-2025 English PDF

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GB/T 33682-2025: General microoganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry
Status: Valid

GB/T 33682: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 33682-2025	199	Add to Cart	3 days	General microoganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry	Valid
GB/T 33682-2017	109	Add to Cart	3 days	General microorganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry	Valid

Similar standards

GB/T 38505   GB/T 38132   GB/T 36081   GB/T 33681   GB/T 33681.1   

Basic data

Standard ID: GB/T 33682-2025 (GB/T33682-2025)
Description (Translated English): General microoganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A40
Classification of International Standard: 07.080
Word Count Estimation: 9,935
Date of Issue: 2025-05-30
Date of Implementation: 2025-09-01
Older Standard (superseded by this standard): GB/T 33682-2017
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 33682-2025: General microoganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 07.080 CCSA40 National Standard of the People's Republic of China Replaces GB/T 33682-2017 Matrix-assisted laser desorption ionization time-of-flight mass spectrometry General principles for methods of identification of microorganisms Released on 2025-05-30 2025-09-01 Implementation State Administration for Market Regulation The National Standardization Administration issued

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 33682-2017 "General rules for identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight mass spectrometry" and Compared with GB/T 33682-2017, in addition to structural adjustments and editorial changes, the main technical changes are as follows. --- Added requirements for pre-treatment methods for different strains (see 8.2); --- Added instrument setting condition requirements (see 8.4.1); --- Added "quality control" requirements (see 8.6); --- Changed the interpretation criteria of “result interpretation” (see 8.8, 8.7 of the.2017 edition); --- Changed the reporting requirements for “Results Report” (Chapter 9, Chapter 9 of the.2017 edition). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document was proposed and coordinated by the National Technical Committee for Biochemical Testing Standardization (SAC/TC387). This document was drafted by. Hebei Provincial Food Inspection Institute, China Testing Technology Research Institute, Heilongjiang Provincial Intellectual Property Protection Center, Tsinghua University University, Guangzhou Huaxin Testing Technology Co., Ltd., Suzhou Shipu Testing Technology Co., Ltd., Henan University, Beijing Institute of Food Science, Nanjing University of Finance and Economics, Hebei Medical University, Beijing Technology and Business University, Zhejiang University of Technology, China Institute of Inspection and Quarantine, Lanzhou Baiyuan Gene Technology Ltd. The main drafters of this document are. Zhang Yan, Zhou Wei, Wang Yi, Zhou Lihua, Peng Liping, Li Yongbo, Kang Wenyi, Sun Yong, Lv Pin, Wendi, Fan Sufang, Tang Xiaozhi, Feng Xiao, Zhang Yalun, Wang Yanbo, Zhou Xuxia, Zhang Rui, Che Tuanjie, Chen Chen, Liao Huayong, Dai Dan, Wang Hui, Liu Shuai, Ren Xiaoyan, Niu Xiangtao, Zou Yan and Zheng Xiaoling. The previous versions of this document and the documents it replaces are as follows. ---First published in.2017 as GB/T 33682-2017; ---This is the first revision. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry General principles for methods of identification of microorganisms

1 Scope

This document specifies the general method for identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This document applies to the matrix-assisted laser desorption ionization time-of-flight mass spectrometry identification of microbial species.

2 Normative references

The contents of the following documents constitute essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB 19489 General requirements for laboratory biosafety

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 The isolated and purified single colony is directly applied to the target plate, and after drying, the matrix is added and mixed. The microbial soluble protein and the matrix form After forming co-crystals, mass spectrometry fingerprints of protein molecules with different mass-to-charge ratios were obtained through a vacuum time-of-flight tube.

4 Abbreviations

The following abbreviations apply to this document. BTS. Bacteria Detection Standard Sample. MALDI-TOFMS. matrix-assisted laser desorption ionization time-of-flight mass spectrometry. MALDI-TOF-TOFMS. matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry. MSP. main spectrum plot.

5 Principle

Common microorganisms are composed of special proteins that are different from other species, and therefore have unique protein fingerprints, which are determined by species. It is determined by the genetic characteristics and is less affected by external environmental conditions. Samples (from clinical samples such as sputum, blood, body fluids, swabs, and food, cosmetics, medicines, and the environment, etc.) are properly processed and cultured in solid After culturing and isolating a single colony on the matrix, the sample was directly spotted onto the target plate and placed into the matrix-assisted laser desorption ionization time-of-flight mass spectrometer. Ionization is separated according to the mass/charge (m/z) ratio, and the collected data is compared with the microbial fingerprint library for identification. ......

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