GB/T 31810-2015 English PDF

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GB/T 31810-2015: Detection and identification of Maize chlorotic mottle virus
Status: Valid

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GB/T 31810-2015	399	Add to Cart	4 days	Detection and identification of Maize chlorotic mottle virus	Valid

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Basic data

Standard ID: GB/T 31810-2015 (GB/T31810-2015)
Description (Translated English): Detection and identification of Maize chlorotic mottle virus��
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 19,116
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-27
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 31810-2015: Detection and identification of Maize chlorotic mottle virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Maize chlorotic mottle virus ICS 65.020.01 B16 National Standards of People's Republic of China Chlorotic mottle virus quarantine testing methods for maize Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine, the People's Republic of China State and Ningbo Entry-Exit Inspection and Quarantine People's Republic of China Shanghai, People's Republic of China Yunnan Entry-Exit Inspection Quarantine Bureau. The main drafters. Zhang Weidong, Zhang Yongjiang, Zhang Huili, Wang Lan, Yang Cuiyun, Liao force, Min Li, Chi Yuan Li, Miao Xu Feng, right Yongbing, Wen Weigang, Chen Qiwen. Chlorotic mottle virus quarantine testing methods for maize

1 Scope

This standard specifies the maize chlorotic mottle virus quarantine and identification methods. This standard applies to maize chlorotic mottle virus may carry the seeds, seedlings and other plant propagation material in maize chlorotic mottle virus detection Phytophthora identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2122 entry and exit quarantine of plants and plant products sampled SN/T 2964-2011 Specification plant virus detection 3 equipment, facilities and Reagents 3.1 Equipment Reader, washer, conventional PCR, fluorescence PCR instrument, micro spectrophotometer, biological safety cabinets, autoclaves, incubators, micro The amount of the balance (the amount of sense. 0.001g), refrigerators, centrifuges, pH meter, water bath, electrophoresis, gel imaging system, the pipette (1000μL, 200μL, 100μL, 10μL, 2.5μL). 3.2 Facilities Isolation greenhouse (10 ℃ ~ 30 ℃). 3.3 Reagents Unless otherwise specified, all reagents were of analytical grade. Reagents and buffers see Appendix B B.1 and Appendix C C.1. Preparation 4 sampling and testing of samples 4.1 Sampling Sampling carried out in accordance SN/T 2122's. 4.2 Preparation of the test sample 4.2.1 seeds Picked at least 500 seeds (emphasis picked malformation, immature seeds) samples over 3% sodium hypochlorite (NaClO) surface disinfection 10min later, the seeds were placed in sterilized soil in a suitable germination temperature (25 ℃ ~ 28 ℃) under the conditions of germination, grow up to 3 ~ 4 true leaves were detected. Select young leaves have chlorotic plants, deformities and other symptoms detected all numbered, randomly selected at least 20 asymptomatic plant samples were mixed sample test. ......

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