GB/T 31800-2015 English PDF

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GB/T 31800-2015: Detection and identification of Plum pox virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 31800-2015	399	Add to Cart	4 days	Detection and identification of Plum pox virus	Valid

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Basic data

Standard ID: GB/T 31800-2015 (GB/T31800-2015)
Description (Translated English): Detection and identification of Plum pox virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 19,176
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-27
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 31800-2015: Detection and identification of Plum pox virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Plum pox virus ICS 65.020.01 B16 National Standards of People's Republic of China Plum pox virus quarantine and identification methods Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Ningbo Entry-Exit Inspection and Quarantine of Guangdong People's Republic of China Bureau, Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Chen Xianfeng, Zhang Huili, Chending Hu, Zhang Ji Hong, Xu Ying, Guo Lixin, Wen Weigang, Yang Hongxia, Du Hongzhong, Shan Wu products. Plum pox virus quarantine and identification methods

1 Scope

This standard specifies the immunology and molecular biology quarantine and identification of plum pox virus. This standard applies to plant material, including quarantine and identification of vegetative propagation material, tissue culture, seeds, flowers and fruits of plum pox virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use - Specification and test methods GB/T 6682 Analysis SN/T 1840 plant virus immune electron microscopy method

3 Equipment and Reagents

3.1 Equipment Ordinary PCR, real-time PCR instrument, microplate reader, electronic balance (1/1000g), electrophoresis, electrophoresis tank, gel imaging system, water Bath, high-speed desktop refrigerated centrifuge, -80 ℃ ultra-low temperature freezer, clean benches, autoclaves, ice maker, microwave, vortex Device. Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and the corresponding RNase-free tip, no RNase centrifuge tubes, PCR tubes and mortar and the like. 3.2 Reagents Unless otherwise specified, all reagents were of analytical grade experiment, test water should be consistent with GB/T 6682 in the relevant regulations. The Trizol, diethyl pyrocarbonate (of DEPC), liquid nitrogen, chloroform, isopropanol, ethanol, [beta] -mercaptoethanol, RNA reverse transcriptase, TaqDNA polymerase, SYBRgreenI, DNA molecular weight standard, ethidium bromide, agarose, bromophenol blue. Double antibody enzyme Linked immunosorbent assay method (DAS-ELISA) detection reagent preparation method, see Appendix B, B.1.

4 identification of quarantine

4.1 Symptom Check Carefully check the plant leaves and flowers, fruit, whether stone faded, ringed, deformities and other suspicious symptoms, if there is a typical symptoms (cf. See Appendix A), can be collected showing symptoms of flower, leaf or fruit subsequent testing. 4.2 pairs of antibody ELISA method (DAS-ELISA) DAS-ELISA specific steps in Appendix B. 4.3 Common RT-PCR detection method Common RT-PCR method to detect the specific steps in Appendix C. ......

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