GB/T 31797-2015 English PDF

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GB/T 31797-2015: Detection and identification of Hop latent viroid
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 31797-2015	299	Add to Cart	3 days	Detection and identification of Hop latent viroid	Valid

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Basic data

Standard ID: GB/T 31797-2015 (GB/T31797-2015)
Description (Translated English): Detection and identification of Hop latent viroid
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,176
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-27
Quoted Standard: GB/T 6682
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies the method for inspection and identification of hop latent viroid. This Standard applies to hops and vegetative propagation material, seeds, hops quarantine and identification of latent viroid on pollen, but also for the Humulus dioica hop quarantine and identification of latent class virus.

GB/T 31797-2015: Detection and identification of Hop latent viroid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Hop latent viroid ICS 65.020.01 B16 National Standards of People's Republic of China Hop latent viroid identification of quarantine Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Ningbo Entry-Exit Inspection and Quarantine Beijing People's Republic of China Bureau, People's Republic of China Xinjiang Exit Inspection and Quarantine, Plant Protection Institute of China Academy of Agricultural Sciences, Shihezi University. The main drafters of this standard. Guo Lixin, Deng Cong Liang, the military section of Victoria, Zhang Xianglin, Shi visit, Jiang Dongmei, Rongde Fu, Liu studies. Hop latent viroid identification of quarantine

1 Scope

This standard specifies the method for inspection and identification of hop latent viroid. This standard applies to hops (Humuluslupulus) and vegetative propagation material, seeds, pollen hop latent viroid on the subject Pest identification, but also for Humulus (Humulusjaponicus) and dioica (Urticadioica) hop latent viroid on the subject Phytophthora identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis 3 equipment, major appliances and Reagents 3.1 Equipment Electronic balance (inductance 0.001g), general balance (inductance 0.1g), high-speed refrigerated centrifuge, small instantaneous centrifuges, ordinary refrigerator, ultra Deep freezer (-80 ℃), ice maker, a vortex, magnetic stirrer, autoclaves, pH meter, PCR instrument, microwave, electrophoresis, electro Swimming tank, gel imaging analysis system, real-time PCR instrument, hybridization oven, plastic film sealing machine, UV cross-linking instrument, camera obscura, a double, adjustable shift Dispenser (2.5μL, 10μL, 20μL, 200μL, 1000μL, 5000μL). 3.2 major appliances RNase-free tip (10μL, 20μL, 200μL, 1000μL, 5000μL), RNase-free tubes (1.5mL, 5mL, 10mL), mortar and pestle, magnetic rack, PCR reaction tube (200μL), real-time fluorescence 96-well plates, bottles hybridization, hybridization membrane, hybridization Bags, X-rays. 3.3 Reagents Unless otherwise specified, all reagents were of analytical grade, test water should be consistent with GB/T 6682 in the relevant regulations. RT-PCR detection test Agents see Appendix B; real-time fluorescent RT-PCR detection reagent in Appendix C; dot blot detection reagent in Appendix D.

4 detects sample preparation

4.1 seeds Random sample of at least 50 seed samples, surface disinfection, lined with absorbent paper placed white porcelain plate or dish, in a suitable hair Germination, until the buds grow under the temperature conditions of the first pair of true leaves (about one week germination time); or directly planted in sterile soil, until long The first pair of true leaves. Take appropriate blade, into a clean mortar quickly ground to a fine powder in liquid nitrogen was added. ......

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