GB/T 31787-2015 English PDF

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GB/T 31787-2015: Molecular detection of Carnation ringspot virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 31787-2015	299	Add to Cart	3 days	Molecular detection of Carnation ringspot virus	Valid

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Basic data

Standard ID: GB/T 31787-2015 (GB/T31787-2015)
Description (Translated English): Molecular detection of Carnation ringspot virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,172
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-27
Quoted Standard: SN/T 1193; SN/T 2122
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This Standard specifies the method of molecular biology carnation ringspot virus, RT-PCR, IC-RT-PCR and real-time RT-PCR and the like. This Standard applies to Carnation ringspot virus detection plant seedlings carried.

GB/T 31787-2015: Molecular detection of Carnation ringspot virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Molecular detection of Carnation ringspot virus ICS 65.020.01 B16 National Standards of People's Republic of China Carnation ringspot virus molecular biology methods Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Tianjin Entry-Exit Inspection and Quarantine Bureau, the Shanghai Institute of Standardization, People's Republic of China Zhuhai CIQ. The main drafters of this standard. Yang Cuiyun at Cui, Wei Yadong, Yang Rui Yu, Zhang Weidong, Guo Jingze, Hupei Long, Cuixue Hui. Carnation ringspot virus molecular biology methods

1 Scope

This standard specifies the biology carnation ringspot virus, RT-PCR, IC-RT-PCR and real-time RT-PCR and other molecular method. This standard applies to Carnation ringspot virus detection plant seedlings carried.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 1193 genetic testing laboratory technical requirements SN/T 2122 entry and exit quarantine of plants and plant products sampled 3 equipment, appliances and reagents 3.1 Equipment PCR, real-time PCR instrument, clean benches, electronic scales (1/10000g), electrophoresis, electrophoresis tank, gel imaging system Systems, water bath, high-speed refrigerated centrifuge, -80 ℃ ultra-low temperature refrigerators, autoclaves, ice maker, microwave, whirlpool oscillator. 3.2 Appliances Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL) and the corresponding RNase-free tips, RNase-off Heart tube, PCR tubes and mortar and the like. 3.3 Reagents RT-PCR reagents (see Appendix B), IC-RT-PCR reagents (see Appendix C).

4 symptom observation and sampling

4.1 Observation of Symptoms MAIN OUTCOME entry quarantine site on plant material damage symptoms of the virus, CRSV Damage symptoms described in Appendix A. 4.2 Sampling Found to have symptoms of the virus damage plant material direct censorship; No symptoms, press SN/T 2122 provisions in sampling, and sent Laboratory virus detection and identification.

5 virus detection

5.1 RT-PCR method The submission plant samples in liquid nitrogen, finely ground plant tissue suspected viral damage symptoms, take 0.1g plant for the extraction of total RNA, then ......

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