GB/T 31789-2015 English PDF

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GB/T 31789-2015: Detection and identification of Phytophthora hibernalis Carne
Status: Valid

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Basic data

Standard ID: GB/T 31789-2015 (GB/T31789-2015)
Description (Translated English): Detection and identification of Phytophthora hibernalis Carne
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 15,112
Date of Issue: 2015-07-03
Date of Implementation: 2015-11-27
Regulation (derived from): National Standard Announcement 2015 No.22
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 31789-2015: Detection and identification of Phytophthora hibernalis Carne

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phytophthora hibernalis Carne ICS 65.020.01 B16 National Standards of People's Republic of China Winter Health quarantine and identification of Phytophthora Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. China Inspection and Quarantine Science Research Institute, Xiamen People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Du Hongzhong, products Wu Shan, Wang Hongyi, Yan Jin, Zhang Qiue. Winter Health quarantine and identification of Phytophthora

1 Scope

This standard specifies the method for inspection and identification of Phytophthora Dongsheng. This standard applies for Winter Health Phytophthora host seedlings, branches, leaves, fruits and entrained soil and medium Dongsheng Phytophthora Identification.

2 apparatus and appliances

2.1 Instrument Biological microscope, clean benches, light incubator, electronic scales (1/1000g), autoclaves, refrigerators, PCR amplification, fluorescence PCR amplification, electrophoresis, gel imager, high-speed refrigerated centrifuge, heated water bath. 2.2 Appliances Beakers, flasks, graduated cylinder, test tubes, petri dishes, alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, plastic pestle Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tank. 3 reagents, materials and media 3.1 Reagents Horses neomycin (pimaricin), ampicillin sodium salt (ampicilin), rifampin sodium salt (rifampicin), PCNB (penta- chloronitrobenzene), calcium carbonate (CaCO3), β- sitosterol (β-sitosterol), tryptophan (tryptophan), sulfur ammonia (thiamine), Calcium chloride (CaCl2 · H2O), sodium hypochlorite (NaClO). Agarose, Tris-HCl, MgCl2, HCl, EDTA (ethylenediaminetetraacetic acid tetrasodium salt), NaOH, NaOAc, KCl, Tris, CTAB (Cetyl trimethyl ammonium bromide), TAE, ethanol (Ethanol), chloroform (Chloroform), isopropyl alcohol (Isopropanol), iso Pentanol (Isoamylalcohol), SYBRGreenI nucleic acid stain, proteinase K, TaqDNA polymerase, dNTPs, DNA molecular Quality standards, PCR primers PHIB1 and PHIB2, real-time PCR primers PH-F and PH-R and TaqMan-MGB probe PH-Pr. 3.2 Material Agar, V8 juice, cedar pine needles (Cedrusdeodara), citrus leaves (Citrusspp.), Carrots, corn oil. 3.3 Medium V8 medium carrots medium CPA, selective medium PARP-V8 and oospores medium specific formula see Appendix Record B.

4 identification of quarantine

4.1 Symptom Check Citrus fruits Is there a key observation brown lesions, whether or withered leaves and twigs; Rhododendron, observe whether the blades have dark brown spots, ......

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