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GB/T 28229-2011 English PDF

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GB/T 28229-2011: Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 28229-2011259 Add to Cart 3 days Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships Valid

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Basic data

Standard ID: GB/T 28229-2011 (GB/T28229-2011)
Description (Translated English): Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C51
Classification of International Standard: 07.100.20
Word Count Estimation: 11,146
Date of Issue: 2011-12-30
Date of Implementation: 2012-05-01
Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the entry and exit of ships' ballast water and diarrhea Escherichia coli (diarrheagenic Escherichia coli) testing procedures and methods. This standard applies to the entry-exit ship ballast water diarrhea detection of Escherichia coli.

GB/T 28229-2011: Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships ICS 07.100.20 C51 National Standards of People's Republic of China Entry and exit of ships ballast water cause diarrhea E. coli Testing method Issued on. 2011-12-30 2012-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The revised standard used by the US Food and Drug Administration (FDA) "bacteriological test manual. diarrhea caused by Escherichia coli" (2009 July) (BAM. DiarrheagenicEscherichiacoli, July2009). The main difference between this standard and the FDA approach are. --- Deletion of some virulence factor detecting step; --- Delete real-time PCR screening step; --- Culture temperature from 35 ℃ ± 0.5 ℃ revised to 36 ℃ ± 1 ℃. This standard was proposed by the State Administration of Quality Supervision, Inspection and Quarantine. The standard by the China Academy of Inspection and Quarantine. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Qinhuangdao Exit Inspection and Quarantine, the Chinese people Exit Inspection and Quarantine of the Republic of Guangzhou. The main drafters of this standard. Wang Lin, Zhang Shun, normally forest, Lijun Cheng, Zhang Yue, Li there, Li Xin, Nie Weizhong, Liao Ruyan, Chen Chuntian, Chi Ying, Liu Endong, Han Hui. Entry and exit of ships ballast water cause diarrhea E. coli Testing method

1 Scope

This standard specifies the entry and exit of ships ballast water cause diarrhea Escherichia coli (diarrheagenicEscherichiacoli) testing procedures And methods. This standard applies to the entry and exit of ships' ballast water cause diarrhea E. coli detected.

2 Definitions

The following terms and definitions apply to this document. 2.1 Diarrhea caused by Escherichia coli diarrheagenicEscherichiacoli Part of the genus Escherichia virulent, can cause diarrhea, collectively known strains of human disease, generally including enteropathogenic Escherichia coli (EPEC), enterohemorrhagic Escherichia coli (EHEC), intestinal invasive Escherichia coli (EIEC ), enterotoxigenic large Escherichia coli (ETEC) and the like.

3 equipment and materials

3.1 Refrigerator. 2 ℃ ~ 8 ℃. 3.2 incubator. 36 ℃ ± 1 ℃, 44 ℃ ± 1 ℃. 3.3 filter collection device bacteria. 3.4 UV lamp. wavelength 365nm. 3.5 sterile conical flask. 500mL. 3.6 sterile pipette. 25mL, accurate to 0.1mL. 3.7 Pipette. 0.1mL, accurate to 0.01mL. 3.8 inoculating loop. 3.9 inoculating needle. 3.10 sterile Petri dish. 90mm. 4 media and reagents 1) 1) recommends using a commercial medium and biochemical reaction tube to ensure stable quality, biochemical reactions can also be used to identify microorganisms automatic biochemical equipment to complete. 4.1 brain heart infusion broth (BHI). 4.2 Double tryptone phosphate broth (dTP). See A.1. 4.3 EHEC enrichment broth (EEB). See A.2. 4.4 eosin methylene blue agar (EMB). See A.3. 4.5 MacConkey agar (MAC). see A.4.
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