GB/T 21813-2008 English PDF

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GB/T 21813-2008: Chemicals -- Honeybees acute contact toxicity test
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 21813-2008	174	Add to Cart	3 days	Chemicals -- Honeybees acute contact toxicity test	Valid

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Basic data

Standard ID: GB/T 21813-2008 (GB/T21813-2008)
Description (Translated English): Chemicals -- Honeybees acute contact toxicity test
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A80
Classification of International Standard: 13.300; 13.020.40
Word Count Estimation: 9,958
Date of Issue: 2008-05-12
Date of Implementation: 2008-09-01
Adopted Standard: OECD No.214-1998, IDT
Regulation (derived from): Announcement of Newly Approved National Standards No. 7, 2008 (No. 120 overall)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the laboratory evaluation of pesticides and other chemicals to bees produce acute contact toxicity test scope, terms and definitions, test purposes, principles, test preparation, test procedure, quality control, data, and reports. This standard applies to laboratory evaluation of pesticides and other chemicals to bees produce acute contact toxicity of size.

GB/T 21813-2008: Chemicals -- Honeybees acute contact toxicity test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals.Honeybees acute contact toxicity test ICS 13.300; 13.020.40 A80 National Standards of People's Republic of China Chemical bee acute contact toxicity test Posted.2008-05-12 2008-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard is identical with the Organization for Economic Co-operation and Development (OECD) Chemicals Test Guide No. 214 (1998) "Acute contact of bees Toxicity test "(in English). This standard made the following editorial changes. --- Added Chapter 1. Range; --- The appendix terms and definitions advanced to Chapter 2; --- Unit "μg/bee" for "μg/bee". This standard by the National Standardization Technical Committee of hazardous chemicals management (SAC/TC251) and focal point. This standard was drafted. Chinese Academy of Inspection and Quarantine, Ningbo Entry-Exit Inspection and Quarantine. The main drafters of this standard. Chen Huiming, Wang Lifeng, Wang Junbing, Yu Wenlian, Zhou Xin, Ma Zhongchun, Lin Zhenxing.

Introduction

This standard specifies the laboratory evaluation of pesticides and other chemical substances on the bees produced acute contact toxicity test methods. This Reference was made to the above method at the Fifth International Conference organized by the International Plant Honey Commission (ICPBR) in the Netherlands in.1993 See the reference [2] for the modification suggestion, reference [3], [4] and [5] at the same time. Chemical bee acute contact toxicity test

1 Scope

This standard specifies the laboratory assessment of insecticides and other chemical substances on the bees acute toxicity of the size of the test range Language and definition, purpose of test, principle, test preparation, test procedure, quality control, data and report. This standard applies to laboratory assessment of pesticides and other chemical substances on the bees acute toxicity.

2 Terms and definitions

The following terms and definitions apply to this standard. 2.1 The test substance has a detrimental effect on the test organism when the test substance is in local contact with the test organism for a maximum of 96 hours. 2.2 The amount of test substance used (μg) is expressed as the mass (μg/bee) of the test substance used for a single bee. 2.3 Refers to the amount of test substance that causes death of 50% of the test organisms when the test organism is in contact with the test substance. Half lethal dose with a single bee by Use the mass of the test substance (μg/bee) to express. For pesticides, the test substance may be an active ingredient, it may contain one or more Preparation of active ingredients. 2.4 The proportion of dead test organisms to the total test organisms.

3 test purposes

Test substance in practical applications, if possible contact with the bees, then assess the toxicity of the test substance, but also need to determine the contact toxicity Sexual size. This standard specifies the test methods for acute contact toxicity of insecticides and other chemical bees, the test results can be used to confirm Subject to test whether the need for further assessment. This standard can be applied in the laboratory - semi-field - field sequence test evaluation of pesticides and chemicals Acute contact hazard of quality bees. Pesticides can be either an active ingredient or a formula-based product during the test. The sensitivity of the bees and the precision of the test procedure should be controlled during the test.

4 principle

The solution of the test substance was dropped onto the bee chest through the carrier, and the experiment was continued for 48 hours to observe the mortality rate of the bees. If in 24h ~ 48h , The bee mortality rate continued to increase, while the mortality rate of bees in the control group remained at an acceptable level, for example, not more than 10% To consider extending the trial to 96h. Mortality was recorded daily and compared with the control group. The results were analyzed and calculated for 24h and 48h Of LD50, as well as LD50 values at 72 h and 96 h after prolonged testing.

5 test preparation

5.1 Bee selection The test bees should be selected from the same population, try to choose the same bee age, feeding the same way, good nutrition, disease resistance, and ......

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