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GB 31610.1-2023 English PDF

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GB 31610.1-2023: (National Food Safety Standards Inspection of Gnatostome nematodes in animal aquatic products and their products)
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB 31610.1-2023179 Add to Cart 3 days (National Food Safety Standards Inspection of Gnatostome nematodes in animal aquatic products and their products) Valid

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Basic data

Standard ID: GB 31610.1-2023 (GB31610.1-2023)
Description (Translated English): (National food safety standards: Inspection of Angiostrongylus cantonensis in animal aquatic products and their products)
Sector / Industry: National Standard
Classification of Chinese Standard: X09
Word Count Estimation: 7,780
Date of Issue: 2023-09-06
Date of Implementation: 2024-03-06
Issuing agency(ies): National Health Commission of the People's Republic of China, State Administration for Market Regulation
Summary: This standard specifies the morphological and PCR detection methods of Angiostrongylus cantonensis larvae in animal aquatic products and their products. This standard is applicable to the inspection of Angiostrongylus cantonensis larvae in animal aquatic products and their products.

GB 31610.1-2023: (National Food Safety Standards Inspection of Gnatostome nematodes in animal aquatic products and their products)


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Standards of People's Republic of China National food safety standards Inspection of Gnatostome nematodes in animal aquatic products and their products Published on 2023-09-06 Implemented on 2024-03-06 National Health Commission of the People's Republic of China Released by the State Administration for Market Regulation National food safety standards Inspection of Gnatostome nematodes in animal aquatic products and their products

1 Scope

This standard specifies the morphological and PCR detection methods of Gnathostoma larvae in animal aquatic products and their products. This standard is applicable to the inspection of Gnatostoma larvae in animal aquatic products and their products.

2 Principles

Gnatostome larvae in animal aquatic products and their products mainly parasitize in the muscles and other tissues of freshwater fish, amphibians and other aquatic products. The parasites are obtained by digesting animal host tissues with pepsin. According to the morphological characteristics of the larvae, the larvae of Gnatomostoma are judged. To detect suspicious parasites The gene fragment of the mitochondrial cytochrome C oxidase subunit I (cox1) of Gnathostomia nematode was over-amplified and sequenced to conduct the analysis of common Gnatomostoma larvae. identification.

3 Instruments and equipment

3.1 Biological microscope. 100×~400×. 3.2 Stereo microscope. 7.5×~150×. 3.3 PCR amplification instrument. 3.4 Gel imaging system. 3.5 Electrophoresis apparatus. 3.6 Constant temperature incubator. 37℃±1℃. 3.7 Magnetic stirrer. 3.8 High-speed centrifuge. speed ≥12000r/min. 3.9 Mesh screen. hole diameter 2mm (10 mesh). 3.10 Conical measuring cup. 1000mL. 3.11 Micropipette. 0.2μL~2.5μL, 1μL~10μL, 10μL~100μL, 100μL~1000μL.

4 Reagents and materials

4.1 Reagents 4.1.1 Hydrochloric acid. 36%~38% HCl solution. 4.1.2 Physiological saline. 0.85% NaCl solution. 4.1.3 1mol/LTris-HCl solution (pH8.0). 4.1.4 0.5mol/LEDTA solution (pH8.0). 4.1.5 10% SDS solution. 4.1.6 5mol/LNaCl solution. 4.1.7 3000U/mg pepsin.
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