GB 31610.4-2023 English PDF

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GB 31610.4-2023: (National food safety standards - Inspection of animal aquatic products and their products for Clonorchis sinensis)
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB 31610.4-2023	159	Add to Cart	3 days	(National food safety standards - Inspection of animal aquatic products and their products for Clonorchis sinensis)	Valid

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Basic data

Standard ID: GB 31610.4-2023 (GB31610.4-2023)
Description (Translated English): (National food safety standards - Inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products)
Sector / Industry: National Standard
Classification of Chinese Standard: X09
Word Count Estimation: 6,628
Date of Issue: 2023-09-06
Date of Implementation: 2024-03-06
Issuing agency(ies): National Health Commission of the People's Republic of China, State Administration for Market Regulation
Summary: This standard specifies the morphological and PCR detection methods for Spirometra mansoni larvae in animal aquatic products and their products. This standard is applicable to the inspection of Sparganoma mansoni tapeworm in animal aquatic products and their products.

GB 31610.4-2023: (National food safety standards - Inspection of animal aquatic products and their products for Clonorchis sinensis)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Standards of People's Republic of China National food safety standards Animal-based aquatic products and their products Testing for Clonorchis sinensis Published on 2023-09-06 Implemented on 2024-03-06 National Health Commission of the People's Republic of China Released by the State Administration for Market Regulation National food safety standards Animal-based aquatic products and their products Testing for Clonorchis sinensis

1 Scope

test method. This standard is applicable to the inspection of Clonorchis sinensis metacercariae in animal aquatic products and their products.

2 Principles

Clonorchis sinensis metacercariae in animal aquatic products and their products mainly parasitize the muscle tissue of freshwater fish and freshwater shrimp. Observed by tabletting method Detect metacercariae or use pepsin to digest the muscle tissue of animal aquatic products and their products to obtain metacercariae. Based on the morphological characteristics of metacercariae, preliminary Determine the type of metacercariae. By amplifying and sequencing the second internal transcribed spacer (ITS2) gene fragment of the ribosomal DNA of Clonorchis sinensis, Identification of metacercariae of Clonorchis sinensis.

3 Instruments and equipment

3.1 Biological microscope. 100×~400×. 3.2 Stereo microscope. 7.5×~150×. 3.3 PCR amplification instrument. 3.4 Gel imaging system. 3.5 Electrophoresis apparatus. 3.6 Constant temperature incubator. 37℃±1℃. 3.7 High-speed centrifuge. speed ≥12000r/min. 3.8 Mesh screen. aperture 0.25mm (60 mesh). 3.9 Conical measuring cup. 1000mL. 3.10 Micropipette. 0.2μL~2.5μL, 1μL~10μL, 10μL~100μL, 100μL~1000μL.

4 Reagents and materials

4.1 Reagents 4.1.1 Hydrochloric acid. 36%~38% HCl solution. 4.1.2 Physiological saline. 0.85% NaCl solution. 4.1.3 1mol/LTris-HCl solution (pH8.0). 4.1.4 0.5mol/LEDTA solution (pH8.0). 4.1.5 10% SDS solution. 4.1.6 5mol/LNaCl solution. ......

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