GB 25535-2010 English PDF

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GB 25535-2010: National food safety standards of food additives gellan gum
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB 25535-2010	209	Add to Cart	3 days	National food safety standards of food additives gellan gum	Valid

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Basic data

Standard ID: GB 25535-2010 (GB25535-2010)
Description (Translated English): National food safety standards of food additives gellan gum
Sector / Industry: National Standard
Classification of Chinese Standard: X40
Classification of International Standard: 67.220.20
Word Count Estimation: 9,966
Date of Issue: 2010-12-21
Date of Implementation: 2011-02-21
Regulation (derived from): Ministry of Health Bulletin No. 19 of 2010
Issuing agency(ies): Ministry of Health of the People's Republic of China
Summary: This Chinese standard applies to Elodea aeruginosa (Pseudomonas elodea) for pure culture fermentation of carbohydrates, processed food additives made ??of gellan gum.

GB 25535-2010: National food safety standards of food additives gellan gum

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National food safety standards of food additives gellan gum National Food Safety Standard Food Additives gellan gum Issued on. 2010-12-21 2011-02-21 implementation National Standards of People's Republic of China People's Republic of China Ministry of Health issued

Foreword

Appendix A of this standard is a normative appendix, Appendix B is an informative annex. National Food Safety Standard Food Additives gellan gum

1 Scope

This standard applies to the Elodea aeruginosa (Pseudomonas elodea) of carbohydrates pure culture fermentation, by adding Food Additives workers made gellan gum.

2 Normative references

The standard file referenced in the application of this standard is essential. For cited documents with dates, only the date of Version applies to this standard. For undated references, the latest edition (including any amendments) applies to this standard.

3 molecular structure and molecular weight

3.1 Molecular Structure The basic unit consists of one molecule of glucuronic acid molecule rhamnose and two molecules of glucose repeating polymeric composition. 3.2 relative molecular mass 4 × 105 ~ 6 × 105 (according to 2007 international relative atomic mass) 4. Technical Requirements 4.1 Sensory requirements. comply with Table 1. Table 1 Sensory requirements Project requires test methods Color white take appropriate sample is placed in a clean, dry white porcelain dish, in natural light Under observe its color and texture. Organization Status powder 4.2 Physical indicators. to comply with Table 2. Table 2. Physical and chemical indicators 4.3 microbial indicators. to comply with Table 3. Item Index Test Method Gellan gum, w /% 85.0 ~ 108.0 Appendix A A.3 Loss on drying, w /% ≤ 15.0 GB 5009.3-2010 a direct drying Lead (Pb)/(mg/kg) ≤ 2 GB 5009.12 Isopropanol b/(mg/kg) ≤ 750 Appendix B a drying temperature and time are 105 ℃ and 2.5h. b limited to non-ethanol processing gellan gum products. Table 3 microbiological indicators Item Index Test Method Cfu/(CFU/g) ≤ 10000 GB 4789.2 Coliform/(MPN/100g) ≤ 30 GB 4789.3 Salmonella 0/25g GB 4789.4 Molds and yeasts/(CFU/g) ≤ 400 GB 4789.15

Appendix A

(Normative) Testing method A.1 General Provisions Unless otherwise indicated in the analysis using only recognized as analytical reagents and GB/T 6682-2008 specified in the water. In the analysis Standard titration solution, impurities measured by standard solution, preparations and products, did not indicate when the other requirements, according to GB/T 601, GB/T 602, the provisions of preparation GB/T 603's. This solution was used in the test does not indicate what is formulated with solvent, it refers to an aqueous solution. A.2 Identification Test A.2.1 Solubility Test Dissolved in water, forming a viscous solution; insoluble in ethanol. A.2.2 calcium gel test 1g samples were dissolved in 99mL water, formulated as a 1% solution. With a mechanical stirrer and a propeller-shaped stirring blades, stirring the Solution 2h (left portion of the solution when used in A.2.3), with a wide bore pipette to take a small amount of the solution, which was moved CaCl2 solution (100g/L), and made I.e. form a gel. A.2.3 sodium gel test A.2.2 formulated to 1% of the sample was added 0.5g NaCl, and the solution is heated to 80 ℃, with constant stirring and at 80 ℃ condition 1min, to stop heating and stirring, cooled to room temperature to form a hard gel. A.3 Determination of cold glue knot A.3.1 Reagents and materials a) diatomaceous earth. chromatography. b) ethanol. c) ethanol. 7822. A.3.2 Instruments and Equipment a) glass filter. b) a dryer (180 mm). A.3.3 Analysis step Weigh about 1.0g HPLC grade diatomaceous earth, placed in a glass filter, uniform paving, 105 ℃ dried 5h, in a desiccator Accurately weighed after cooling. It weighs about 0.2g after drying (105 ℃, 2.5h) sample (accurate to 0.001g), add 50mL of water at about 80 ℃ Water bath and stirred to dissolve 30min. Added 200mL pre-heated to 60 ℃ ~ 70 ℃ absolute ethanol, mixed, allowed to stand for 12h, spend Said glass containing celite filter, and the filter residue was washed 5 times with ethanol, washed 3 times before each dosage 20mL, twice with The amount of each wash 10 mL. The residue at 105 ℃ drying 5h, accurately weighed after cooling in a desiccator. A.3.4 Calculation Results Gellan gum content X1 according to formula (A.1) Calculated. 1 × = m mX% (A.1) Where. X1-- gellan gum content,%; m1-- residue mass in grams (g); m0-- sample mass, in grams (g). The results parallel arithmetic mean of the measurement results shall prevail. Twice in the same condition absolutely independent determination results The difference is not more than 2% of the arithmetic mean.

Appendix B

(Informative) Determination of isopropanol B.1 Reagents and materials a) isopropanol. chromatography. b) tert-butanol. HPLC grade. B.2 instruments and equipment a) Gas chromatograph. with hydrogen flame ionization detector. b) Column. 1.8m × 2.3mm (id) stainless steel column or an equivalent efficacy column filled 0.2mm ~ 0.15 mm of Porapak QS Fillers or other equivalents. B.3 reference chromatographic conditions a) carrier gas. helium. b) flow rate. 80 mL/min. c) Inlet temperature.200 ℃. e) Column temperature. 165 ℃. f) Detector temperature.200 ℃. g) Injection volume. 5μL. h) isopropanol retention time of approximately 2min, t-butanol retention time of approximately 3min. B.4 analysis step B.4.1 Preparation of standard solutions B.4.1.1 isopropanol (IPA) standard solution Accurately weigh 500.0 mg of isopropanol, transferred to a 50mL volumetric flask, and diluted with water to volume, and mix. Draw 10mL The solution was transferred to a 100mL volumetric flask, and diluted with water to volume, and mix. B.4.1.2 t-butanol (TBA) standard solution Accurately weigh 500.0 mg of tert-butanol and transferred to a 50mL volumetric flask, and diluted with water to volume, and mix. Draw 10mL The solution was transferred to a 100mL volumetric flask, and diluted with water to volume, and mix. B.4.1.3 mixed standard solution Pipette 4mL isopropyl alcohol (IPA) and tert-butyl alcohol (TBA) standard solution was transferred to a 125 mL Erlenmeyer flask, add water to dilute Diluted to about 100mL, and mix. Each ml of this solution contains about 40 μg and 40 μg of isopropanol tert-butanol. B.4.2 samples were prepared In 200 mL of water containing 1000 mL round-bottomed distillation flask, 1mL Suitable defoamers (such as Dow-Corning G-10 or equivalent Thereof), and dispersed. Join accurately weighed sample of about 5g gellan gum, shaking 1h. The bottle was connected to a distillation column, adjust the temperature, So that the foam can not enter the column, then the distillate of about 100mL. Added 4.0mL of tert-butanol in the distillate (TBA) standard solution to prepare test Sample solution. B.4.3 Determination 5μL injection mixed standard solution was measured at reference chromatographic conditions, record isopropanol peak areas AIPA, tert-butyl alcohol Peak areas ATBA, calculate the relative correction factor f (f = AIPA/ATBA). Similarly, injection 5μL sample solution, under reference chromatographic conditions were measured peak area values recorded isopropanol aIPA, tert-butanol The peak areas aTBA. B.5 Calculation Results Isopropanol content X according to the formula (B.1) Calculated. maf aX TBA IPA ×× × = 4000 (B.1) Where. X-- isopropyl alcohol content in milligrams per kilogram (mg/kg); aIPA-- sample liquid chromatographic isopropanol mean peak area; f-- relative correction factor (f = AIPA/ATBA); aTBA-- sample liquid t-butanol mean peak area; m-- saying that the quality of the sample taken, in grams (g). ......

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