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SN/T 4612-2016: Detection of viral hepatitis C by real-time fluorescence RT-PCR at frontier ports
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Detection of viral hepatitis C by real-time fluorescence RT-PCR at frontier ports
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SN/T 4612-2016
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Basic data | Standard ID | SN/T 4612-2016 (SN/T4612-2016) | | Description (Translated English) | Detection of viral hepatitis C by real-time fluorescence RT-PCR at frontier ports | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Word Count Estimation | 6,670 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.438 | | Issuing agency(ies) | General Administration of Customs | SN/T 4612-2016: Detection of viral hepatitis C by real-time fluorescence RT-PCR at frontier ports
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(Detection of Hepatitis C Virus by RT - PCR at National Port)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Frontier port hepatitis C virus fluorescence
RT-PCR detection method
Published on.2016-08-23
2017-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Entry and exit inspection and quarantine
Industry Standard
Frontier port hepatitis C virus fluorescence
RT-PCR detection method
China Standard Press Publishing
First edition, February.2018
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Sichuan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Entry and Exit Inspection of the People's Republic of China
Epidemic situation.
The main drafters of this standard. Tian Lubo, Fan Xuejun, Shi Lei, Gao Guolong, Chen Xiaoyu, Shi Ying.
Frontier port hepatitis C virus fluorescence
RT-PCR detection method
1 Scope
This standard specifies the subject of hepatitis C virus fluorescent RT-PCR detection at the border port, the collection, transportation and preservation of specimens, and the detection process.
Preface and results report.
This standard applies to the laboratory testing of hepatitis C virus in entry and exit personnel at border ports.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
SN/T 1193 Genetic Testing Laboratory Technical Requirements
Application of Polymerase Chain Reaction (PCR) Technology in WS/T 230 Clinical Diagnosis
List of pathogenic microorganisms transmitted from humans (Ministry of Health,.2006)
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Hepatitis C virus viralhepatitisC
It belongs to the Flaviviridae family and is an enveloped RNA virus.
Note. It is mainly caused by blood, sexual life, vertical transmission of mother and baby, causing hepatitis C virus, in which blood transmission is the main mode of transmission of hepatitis C virus. Type C
Hepatitis is a global epidemic that can lead to chronic inflammation, necrosis and fibrosis in the liver. Some patients can develop cirrhosis or even hepatocellular carcinoma. Clinically acute
Hepatitis C manifests as elevated alanine aminotransferase, nausea, loss of appetite, yellow urine, yellow eyes and other symptoms. Chronic hepatitis C is characterized by fatigue, poor appetite, and abdomen.
Bulging and so on.
3.2
Fluorescence RT-PCR real-timefluorescence RT-PCR
On the basis of conventional RT-PCR, a specific fluorescent probe is added, which is an oligonucleotide labeled with one on each side.
A fluorescent reporter group and a fluorescent quenching group. When the probe is intact, the fluorescent signal emitted by the reporter group is absorbed by the quenching group, PCR
During amplification, the probe is hydrolyzed by the 5'-3' exonuclease activity of the Taq enzyme to separate the fluorescent reporter group from the fluorescence quenching group.
The fluorescence monitoring system can receive fluorescent signals. Every time a PCR cycle is passed, the fluorescent signal is the same as the target segment, and there is a synchronization.
In the process of exponential growth, the intensity of the signal is proportional to the nucleic acid content of the test object, and the content of the test object can be calculated according to the mathematical model.
3.3
Ct value cyclethreshold
In the fluorescence RT-PCR amplification process, the value of the fluorescent signal within the range of the exponential growth region is called the threshold (threshold).
The number of amplification cycles elapsed when the fluorescent signal of the product increases to a set threshold is referred to as the Ct value.
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