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SN/T 4097-2015: Real-time fluorescent PCR quarantine protocol for Perkinsus sp.in shellfish
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4097-2015 | English | 189 |
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Real-time fluorescent PCR quarantine protocol for Perkinsus sp.in shellfish
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SN/T 4097-2015
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PDF similar to SN/T 4097-2015
Basic data | Standard ID | SN/T 4097-2015 (SN/T4097-2015) | | Description (Translated English) | Real-time fluorescent PCR quarantine protocol for Perkinsus sp.in shellfish | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 8,829 | | Date of Issue | 2015-02-09 | | Date of Implementation | 2015-09-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | State-Quality-Inspection-Accreditation [2015] 59 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the real-time PCR detection method shellfish centrist piano insects. This standard applies to shellfish tissue centrist piano insect detection. | SN/T 4097-2015: Real-time fluorescent PCR quarantine protocol for Perkinsus sp.in shellfish---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Shellfish piano insect send real-time PCR detection method)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Shellfish send piano insect real-time PCR detection method
Issued on. 2015-02-09
2015-09-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters. Zhang Xue, Zhao Huijun, Li Ye, LI Zhen-Rong, Jia Yun.
Shellfish send piano insect real-time PCR detection method
1 Scope
This standard specifies the real-time PCR detection method shellfish centrist piano insects.
This standard applies to shellfish tissue centrist piano insect detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Real-time fluorescence PCR real-timefluorescentPCR
On the basis of the ordinary on the polymerase chain reaction to add a specific fluorescent probe which is an oligonucleotide labeled at both ends of a
Reports a fluorophore and quencher fluorophore. When the probe is intact, the fluorescence signal emitted by the reporter group is absorbed by the quencher; PCR
Amplification, Taq polymerase 5'-3 'exonuclease activity of the enzyme degradation of the probe, so that the reporter group and quencher separate fluorescence monitoring system in order to
Receiving the fluorescence signal, per amplify a DNA strand, there is formed a fluorescent molecule, a cumulative PCR product shaped fluorescent signal
A fully synchronized, real-time quantitative achieve the purpose.
3.2
Ct values cyclethreshold
When the number of cycles for each reaction tube fluorescent signal reaches the threshold set by experienced, also known as Cp values.
4 Reagents and materials
4.1 1mol/LTris · Cl (pH8.0), 0.5mol/LEDTA solution (pH8.0), SDS (10%). Solution preparation see Appendix A.
4.2 proteinase K solution (20mg/mL).
4.3 Tris- saturated phenol.
4.4 chloroform.
4.5 isoamyl alcohol.
4.6 ethanol.
4.7 saline.
4.8 10 × PCRbuffer, 25mmol/LMgCl2,2.5mmol/LdNTP, 5U/μLTaq enzyme.
4.9 10μmol/L upstream primer, 10μmol/L downstream primer, 10μmol/L probe.
4.10 TE buffer (pH8.0).
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