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SN/T 4002-2014: Determination of silybin in health foods for export - HPLC method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4002-2014 | English | 369 |
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Determination of silybin in health foods for export - HPLC method
| Valid |
SN/T 4002-2014
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Standard similar to SN/T 4002-2014 SN/T 5268 Basic data | Standard ID | SN/T 4002-2014 (SN/T4002-2014) | | Description (Translated English) | Determination of silybin in health foods for export - HPLC method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 14,161 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies a high performance liquid chromatographic method silibinin health foods. This standard applies to capsules and tablets class DETERMINATION silybin care. | SN/T 4002-2014: Determination of silybin in health foods for export - HPLC method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Export health foods silibinin High performance liquid chromatography)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of export health food in the silibinin
High Performance Liquid Chromatography
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hunan Exit Inspection and Quarantine, Inspection and Quarantine Institute of Science and Technology in Hunan Province, China
Xiamen People's Republic of Exit Inspection and Quarantine.
The main drafters of this standard. Mei-ling, Shiya Mei, Jiao Yanna, Liang Jingtao, Fushan Liang, Yanhong Fei, Zhang Ying, Huang Zhiqiang.
Determination of export health food in the silibinin
High Performance Liquid Chromatography
1 Scope
This standard specifies a high performance liquid chromatographic method silibinin health foods.
This standard applies to capsules and tablets class DETERMINATION silybin care.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
Samples silibinin methanol ultrasonic extraction, HPLC, external standard.
4 Reagents and materials
Except where noted, all reagents were of analytical grade water as a water line with GB/T 6682 regulations.
4.1 Methanol. HPLC grade.
4.2 phosphoric acid.
4.3 sodium dihydrogen phosphate.
4.4 phosphoric acid solution (0.2mol/L). Pipette 1.33mL phosphate in 100mL volumetric flask, and dilute with water to volume.
Sodium dihydrogen phosphate 4.5 (0.5mol/L). said take 6.0g of sodium dihydrogen phosphate in 100mL flask, dissolved in water and dilute to
Scale.
4.6 silibinin Standards (CAS Number. 22888-70-6, molecular formula. C25H22O10). silibinin A and silibinin B mixture, pure
Greater than equal to 98%.
4.7 silibinin standard stock solution. Weigh water silibinin standard (4.6) amount, dissolved in methanol to prepare a concentration of 1.0mg/mL of
Standard stock solution, 4 ℃ stored in the dark.
4.8 silibinin standard solution. draw the right amount of silibinin standard stock solution (4.7), diluted to the appropriate concentration range mark with methanol
Quasi working solution, preparation before use.
5 Instruments
5.1 HPLC - with diode array detector.
5.2 ultrasonic cleaner.
5.3 Analysis of balance. a sense of the amount of 0.0001g and 0.01g.
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