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SN/T 3033-2018 English PDF

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SN/T 3033-2018: (Method for authenticating molecular biology of export bird's nest. Real-time fluorescent PCR method and two-dimensional electrophoresis method)
Status: Valid

SN/T 3033: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 3033-2018English399 Add to Cart 4 days [Need to translate] (Method for authenticating molecular biology of export bird's nest. Real-time fluorescent PCR method and two-dimensional electrophoresis method) Valid SN/T 3033-2018
SN/T 3033-2011English519 Add to Cart 3 days [Need to translate] Analysis of edible birds nest product by molecular biological methods. Real-time PCR and 2DGE Obsolete SN/T 3033-2011

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Basic data

Standard ID SN/T 3033-2018 (SN/T3033-2018)
Description (Translated English) (Method for authenticating molecular biology of export bird's nest. Real-time fluorescent PCR method and two-dimensional electrophoresis method)
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X04
Word Count Estimation 16,120
Date of Issue 2018-03-12
Date of Implementation 2018-10-01
Older Standard (superseded by this standard) SN/T 3033-2011
Regulation (derived from) National Quality Inspection (2018) 76
Issuing agency(ies) General Administration of Customs

SN/T 3033-2011: Analysis of edible birds nest product by molecular biological methods. Real-time PCR and 2DGE


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Analysis of edible bird "s nest product by molecular biological methods.Real-time PCR and 2DGE People's Republic of China Entry-Exit Inspection and Quarantine Standards Identification of the molecular biology of bird's nest Real-time PCR method and two-dimensional electrophoresis method Issued on. 2011-09-09 2012-04-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Guangdong CIQ. The main drafters of this standard. Chen Ying, Wu Yajun, Huang Huajun, Wang Bin, Yang Hairong, Zhao Yongsheng. Identification of the molecular biology of bird's nest Real-time PCR method and two-dimensional electrophoresis method

1 Scope

This standard specifies the method of identifying molecular biology bird's nest. Qualitative detection and identification of bird's nest protein and other proteins of biological origin This standard applies to nest components, wherein the PCR method is suitable for Yan Qualitative detection nest components, two-dimensional electrophoresis method for the detection of bird's nest protein and other proteins of biological origin, but not for carrageenan, agar And other protein-free ingredients or low protein content of the sample is detected.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 27403 laboratory quality control of food molecular biology standardized testing 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this document. 3.1.1 SYBRgreen real-time fluorescence PCR SYBRgreenreal-timePCR Join SYBRgreen fluorescent dye in a PCR reaction system. The dye binds only double stranded nucleic acid molecule. PCR amplification, with the The target number of fragments increases, the number of dye molecules also increases, to take advantage of the accumulation of the fluorescent signal in real-time monitoring of the entire PCR amplification Increasing process. 3.1.2 Two-dimensional electrophoresis 2DGE Two-dimensional electrophoresis is a charge based on the characteristics and relative molecular mass protein to protein separation techniques. First, based on the inherent power of protein Netherlands, by isoelectric focusing were the first to separate, and then based on the relative molecular mass protein electrophoresis by SDS perpendicular to a second protein Separation. 3.2 Acronyms The following abbreviations apply to this document. DNA (deoxyribonuleicacid). DNA dATP (deoxyadenosinetriphosphate). triphosphate dCTP (deoxycytidinetriphosphate). deoxycytidine triphosphate dGTP (deoxyguanosinetriphosphate). deoxyguanosine triphosphate

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