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SN/T 1174-2003 English PDF

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SN/T 1174-2003: Antibody detection methods for simian retrovirus type D
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PDF similar to SN/T 1174-2003


Standard similar to SN/T 1174-2003

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Basic data

Standard ID SN/T 1174-2003 (SN/T1174-2003)
Description (Translated English) Antibody detection methods for simian retrovirus type D
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Word Count Estimation 11,157
Date of Issue 2003-03-17
Date of Implementation 2003-09-01
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the monkey type D retrovirus disease antibody detection methods and results of determination. This standard applies to the monkey type D retrovirus disease quarantine.

SN/T 1174-2003: Antibody detection methods for simian retrovirus type D

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Antibody detection methods for simian retrovirus type D Book of the People's Republic of China Entry and Exit Inspection and Quarantine Monkey D-type retrovirus antibody detection method Released on.2003-03-17 2003-09-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Jia Jianjun, Zhou Xiaoli, Xu Zizhong, Ying Xuesong. This standard is the industry standard for inspection and quarantine issued for the first time. Monkey D-type retrovirus antibody detection method

1 Scope

This standard specifies the detection method and result determination of monkey D-type retroviral disease antibody. This standard applies to the quarantine of monkey D-type retrovirus disease.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)

3 Abbreviations

The following abbreviations apply to this standard. 3.1 CPE Caused by cytopathic effects. 3.2 TCID50 Half of the tissue culture infects. 3.3 ELISA Enzyme-linked immunosorbent assay. 3.4 Western blotting test. 3.5 SDS-PAGE SDS polyacrylamide gel electrophoresis.

4 Principle

4.1 ELISA principle The basic principle of the ELISA method is that the enzyme molecule is covalently bound to the antibody or anti-antibody molecule, and this binding does not change the immunology of the antibody. The properties do not affect the biological activity of the enzyme. The enzyme-labeled antibody can specifically bind to an antigen or antibody adsorbed on a solid phase carrier. Hehe. After the substrate solution is added dropwise, the substrate can be converted from a colorless reduced form to a colored oxidized form by an enzyme. Color reaction. Therefore, the color reaction of the substrate can be used to determine whether there is a corresponding immune response, the depth of the color reaction and the corresponding antibody in the sample. Or the amount of antigen is proportional. This color reaction can be quantitatively determined by an ELISA detector, thus the sensitivity of the enzyme chemical reaction. The specificity of the antigen-antibody reaction combines to make the ELISA method a both specific and sensitive assay.

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