HOME   Cart(0)   Quotation   About-Us Policy PDFs Standard-List
www.ChineseStandard.net Database: 189759 (19 Oct 2025)

HYT216-2017 English PDF

US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
HYT216-2017: Determination of tetrodotoxin
Status: Valid
Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
HY/T 216-2017English279 Add to Cart 3 days [Need to translate] Determination of tetrodotoxin Valid HY/T 216-2017

PDF similar to HYT216-2017


Standard similar to HYT216-2017

HJ 491   GB/T 17136   HY/T 0319   

Basic data

Standard ID HY/T 216-2017 (HY/T216-2017)
Description (Translated English) Determination of tetrodotoxin
Sector / Industry Marine Industry Standard (Recommended)
Classification of Chinese Standard Z19
Classification of International Standard 07.060; 13.020
Word Count Estimation 12,122
Date of Issue 2017-02-21
Date of Implementation 2017-06-01
Quoted Standard GB/T 6379.2
Regulation (derived from) State Oceanic Administration Notice No. 2 of 2017 (No. 34)
Issuing agency(ies) State Oceanic Administration
Summary This standard specifies the high performance liquid chromatography (HPLC) method for the detection of tetrodotoxin in shellfish and fish (excluding its products). This standard applies to shellfish, fish (excluding its products) determination of tetrodotoxin. The limit of detection of tetrodotoxin in this standard is 0.1 mg/kg and the limit of quantification is 0.25 mg/kg.

HYT216-2017: Determination of tetrodotoxin

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of tetrodotoxin ICS 07.060;13.020 Z19 People's Republic of China Marine Industry Standard Tetrodotoxin detection method 2017-02-21 released 2017-06-01 Implementation Issued by the State Oceanic Administration

Table of contents

Preface Ⅲ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Principle 1 5 Reagent 1 6 Instruments and equipment 2 7 Preparation and storage of samples 2 8 Sample determination 3 9 Presentation and calculation of results 3 10 Precision 4 11 Experimental notes 4 Appendix A (informative appendix) High performance liquid chromatogram of tetrodotoxin standard sample and positive sample 5 Appendix B (Informative Appendix) Standard Curve Chart 6 of Tetrodotoxin Standard Solution Appendix C (informative appendix) Recovery rate data 7 Appendix D (informative appendix) reproducibility data 9

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Third Institute of Oceanography, State Oceanic Administration. This standard is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283). This standard was drafted by the Third Institute of Oceanography, State Oceanic Administration, and Ocean Standard Metrology Center, State Oceanic Administration. The main drafters of this standard. Chen Weizhu, Yi Ruizao, Yuan Lingling, Hong Bihong, Zhang Yiping, Hong Zhuan, Jin Wenhui. Tetrodotoxin detection method Warning---Because tetrodotoxin is a highly toxic solid powder, you should wear a mask and gloves when using it, avoid inhalation, and prepare a solution Avoid direct contact with the human body. If you accidentally touch the skin or eyes, rinse with plenty of water. In severe cases, send it immediately hospital.

1 Scope

This standard specifies the high performance liquid chromatography fluorescence detection method for tetrodotoxin in shellfish and fish (excluding its products). This standard applies to the determination of tetrodotoxin in shellfish and fish (excluding its products). The detection limit of this standard for tetrodotoxin is 0.1mg/kg, and the limit of quantification is 0.25mg/kg.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6379.2 Accuracy of measurement methods and results (accuracy and precision) Part 2.Determine the repeatability of standard measurement methods Basic methods of sex and reproducibility

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Tetrodotoxin; TTX The blood and internal organs contain the most exogenous neurotoxins in some fish of the puffer family (such as the oriental puffer). Anesthesia in small doses Arthralgia and analgesic effects, a larger dose can be fatal. The structural formula is as follows.

4 Principle

The tetrodotoxin is soluble in weak acid for extraction. Tetrodotoxin can produce fluorescent derivatives in strong alkali. After derivatization-high performance liquid chromatography fluorescence method for detection, qualitative analysis according to retention time, quantitative analysis of peak area.

5 Reagents

Unless otherwise specified, only reagents and ultrapure water or equivalent purity water confirmed to be analytically pure are used in the analysis.

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of HYT216-2017_English be delivered?

Answer: Upon your order, we will start to translate HYT216-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of HYT216-2017_English with my colleagues?

Answer: Yes. The purchased PDF of HYT216-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.