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GB/T 45000-2024: Surface active agents - Determination of components of sucrose fatty acid esters - Liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 45000-2024 | English | 119 |
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Surface active agents - Determination of components of sucrose fatty acid esters - Liquid chromatography
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GB/T 45000-2024
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Basic data | Standard ID | GB/T 45000-2024 (GB/T45000-2024) | | Description (Translated English) | Surface active agents - Determination of components of sucrose fatty acid esters - Liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G72 | | Classification of International Standard | 71.100.40 | | Word Count Estimation | 6,611 | | Date of Issue | 2024-11-28 | | Date of Implementation | 2025-06-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 45000-2024: Surface active agents - Determination of components of sucrose fatty acid esters - Liquid chromatography
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Surface active agents - Determination of components of sucrose fatty acid esters - Liquid chromatography
Composition Analysis of Sucrose Fatty Acid Esters as Surfactants
Liquid chromatography
Surface active agents-Determination of components of sucrose fatty acid
esters-Liquid chromatography
ICS 71.100.40
CCS G 72
National Standard of the People's Republic of China
Released on 2024-11-28
2025-06-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by China Light Industry Federation.
This document is under the jurisdiction of the National Technical Committee on Standardization of Surfactants and Detergents (SAC/TC 272).
This document was drafted by. Hangzhou Ruilin Chemical Co., Ltd., Zhejiang Product Quality and Safety Science Research Institute, China Daily Chemical Research Institute
Co., Ltd., Yiwu Standardization Research Institute, Zhejiang Wufangzhai Industrial Co., Ltd., Hangzhou Dakang Pickled Food Co., Ltd., Shandong Fu
Reda Biotech Co., Ltd., Yunnan Beitani Biotech Group Co., Ltd., Nanjing Well Biotech Co., Ltd., Guangdong Zhong
Kehongtai New Materials Co., Ltd., Guangzhou Kema Biotechnology Co., Ltd., Dongguan Lidun Detergents Industrial Co., Ltd., Jiangsu Han
Bang Technology Co., Ltd., Zhejiang University of Technology.
The main drafters of this document are. Jiang Kan, Zhou Jiang, Yang Liang, Chen Keshu, Chen Xiaozhen, Wang Qian, Hu Jingjing, Zhang Xinxin, Chen Zhaogui,
Yan Hongbing, Xia Shengqin, Niu Canjie, Ding Yicheng, Zhang Donglei, Zhang Shuqi, Lu Caiyuan, Chen Liqun, Ren Xiaoze, Chen Cheng, Yuan Shuying,
Wang Yan'e, Dai Peixuan, Zou Jianying, Wu Xiaoqun, Duo Xiu, Yuan Jun, Liu Qianfang, Chen Yongfu, Zhang Qianyao, Zhang Zheng, Yao Chenzhi, Wang Qunjie,
Liu Fei, Wang Feifei, Qi Nan, Wang Meiling, Peng Anzhe, Ke Zhiwei, Zhang Dabing.
Composition Analysis of Sucrose Fatty Acid Esters as Surfactants
Liquid chromatography
1 Scope
This document describes a liquid chromatography method for the compositional analysis of sucrose fatty acid esters of surfactants.
This document applies to the composition analysis of synthetic sucrose fatty acid ester products.
2 Normative references
This document has no normative references.
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principles of the method
Sucrose fatty acid esters are composed of monoesters, diesters, triesters, tetraesters and other products with different degrees of substitution and unreacted fatty acids. The samples were dissolved and filtered.
Afterwards, sucrose fatty acid esters and fatty acids with different degrees of substitution were separated and detected by high performance liquid chromatography, and quantified by area normalization method.
5 Reagents and Materials
5.1 Tetrahydrofuran. chromatographic grade.
5.2 Sucrose fatty acid esters (CAS. 37318-3-3) Reference substance. purity not less than 95%.
5.3 Preparation of sucrose fatty acid ester reference solution. Weigh 0.125 g (accurate to 0.001 g) of reference substance (5.2) into a 25 mL volumetric flask.
Add an appropriate amount of tetrahydrofuran (5.1) to dissolve, sonicate for 1 min, and dilute to volume with tetrahydrofuran (5.1).
Filter through a microporous membrane and keep the filtrate for later use.
6 Instrument and chromatographic reference conditions
6.1 Instruments
6.1.1 High performance liquid chromatograph. equipped with a differential refractive index or evaporative light scattering detector and a column thermostat system.
6.1.2 Analytical balance. accuracy 0.1 mg.
6.1.3 Micro-injector. 10 μL.
6.1.4 Organic phase microporous membrane. 0.45 μm.
6.1.5 Ultrasonic generator. Ultrasonic power shall not be less than 700 W, and the temperature control range shall be 20 ℃ ~ 80 ℃.
6.2 Liquid chromatography reference conditions
6.2.1 Chromatographic columns. 4 high-efficiency gel permeation chromatography columns (SuperH2500, 6.0 mm × 150 mm, 3 μm) connected in series, or equivalent.
6.2.2 Column temperature. 40 ℃.
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