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GB/T 34797-2017: Quality technical requirements of primers and probes
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| GB/T 34797-2017 | English | 259 |
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Quality technical requirements of primers and probes
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GB/T 34797-2017
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Basic data | Standard ID | GB/T 34797-2017 (GB/T34797-2017) | | Description (Translated English) | Quality technical requirements of primers and probes | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G66 | | Classification of International Standard | 07.080 | | Word Count Estimation | 13,130 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34797-2017: Quality technical requirements of primers and probes---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quality technical requirements of primers and probes
ICS 07.080
G66
National Standards of People's Republic of China
Nucleic acid primer probe quality technical requirements
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized.
This standard drafting unit. China Institute of Tested Technology Institute of Biology, China Institute of Test Technology, Suzhou Jin Wei-biotechnology
Limited, Shenzhen Huada Gene Institute, Shenzhen Institute of Measurement and Quality Inspection.
The drafters of this standard. Zhou Lihua, Li Huaping, Ma Lixia, Liu Mingdong, Hao Junzheng, Jiang Hui, Wang Zhi, Ye Deping, Shi Xiefei, Yang Guowu,
Tan peace.
Nucleic acid primer probe quality technical requirements
1 Scope
This standard specifies the quality of DNA primer probe evaluation criteria, technical requirements, test methods, packaging, transport and storage requirements.
This standard applies to DNA primer probes and other oligonucleotide products quality evaluation and testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 Packaging - Pictorial signs
GB/T 6682 analytical laboratory water specifications and test methods
GB/T 19495.1 Genetically Modified Products General Requirements and Definitions
GB/T 30988 Polyphenols genomic DNA extraction purification and testing methods
3 Terms and definitions
GB/T 19495.1 defined and the following terms and definitions apply to this document.
3.1
Oligonucleotide
A linear polynucleotide fragment of two to thirty nucleotide residues joined by phosphodiester bonds. Oligonucleotides can be self-assembled by the instrument
Into it, it can be used as DNA synthesis primers, gene probes.
NOTE In the use of this term, the number of nucleotide residues is not strictly defined. In many documents, the number of nucleotide residues that contain thirty or more nucleotide residues
Polynucleotide molecules are also referred to as oligonucleotides.
3.2
Primer primer
Synthesized two oligonucleotide sequences, one end of the target gene with a DNA template strand complementary to the other segment and the target gene
The other DNA template strand on the other end is complementary.
3.3
Probe probe
A nucleic acid sequence complementary to the gene of interest with a test signature and sequence known.
3.4
Custom sequence customsequence
Customer supplied DNA sequences and synthesis requirements and other information.
3.5
Custom primers customprimer
Customer-supplied DNA primer sequences and synthesis requirements.
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