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GB/T 34795-2017: Determination of the activity of transglutaminase
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| GB/T 34795-2017 | English | 109 |
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Determination of the activity of transglutaminase
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GB/T 34795-2017
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Basic data | Standard ID | GB/T 34795-2017 (GB/T34795-2017) | | Description (Translated English) | Determination of the activity of transglutaminase | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G66 | | Classification of International Standard | 07.080 | | Word Count Estimation | 5,568 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34795-2017: Determination of the activity of transglutaminase---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of transglutaminase
ICS 07.080
G66
National Standards of People's Republic of China
Detection of transglutaminase activity
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized.
This standard was drafted by Shenzhen Institute of Measurement and Quality Inspection, China Institute of Testing Technology, Sichuan Province Health and Family Planning Supervision
Law Enforcement Corps, Shenzhen Polytechnic.
The main drafters of this standard. Zhang Shiwei, Lai Xin Tian, Lu Jie, Wang Shifeng, Zhou Lihua, Lin Lin, Dong Dong, Ye Xiuling, Lan Quan Xue,
Pan Lanfang, Yang Guowu.
Detection of transglutaminase activity
1 Scope
This standard specifies the source of Streptomyces mobaraensis and guinea pig (Caviaporcellus) source of ammonia
Amyl transaminase activity assay.
This standard applies to biochemical reagents, industrial products in the determination of transglutaminase activity.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Transglutaminase activityunit
N-benzyloxycarbonyl-L-glutamylglycine (CBZ-Gln-Gly, CAS. 6610-42-0) can be catalyzed per minute at 37 ° C,
The amount of enzyme required to form 1.0 μmol of hydroxamate with hydroxylamine is 1 unit in U.
4 principle
Transglutaminase can catalyze the formation of N-benzyloxycarbonyl-L-glutamyl glycine and hydroxylamine L-glutamic acid-γ-monohydroxamic acid, L-glutamine
Acid-γ-monohydroxamic acid can be developed by ferric chloride, with a characteristic absorption peak at a wavelength of 525 nm. Therefore, by external standard method
The activity of L-glutamic acid-γ-monohydroxamic acid was measured as the amount of enzyme produced.
5 equipment and appliances
5.1 pH meter.
5.2 electronic balance. Feel 0.0001g.
5.3 Spectrophotometer.
5.4 1cm quartz cuvette.
5.5 constant temperature water bath. Temperature control accuracy of ± 0.5 ℃.
5.6 Centrifuge.
6 Reagents
The reagents used in this standard are of analytical grade, water is in line with GB/T 6682 provisions of the two water.
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