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GB/T 34794-2017: Guidelines for DNA gel purification kit
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 34794-2017 | English | 159 |
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Guidelines for DNA gel purification kit
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GB/T 34794-2017
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Basic data | Standard ID | GB/T 34794-2017 (GB/T34794-2017) | | Description (Translated English) | Guidelines for DNA gel purification kit | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G66 | | Classification of International Standard | 07.080 | | Word Count Estimation | 8,873 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34794-2017: Guidelines for DNA gel purification kit---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Guidelines for DNA gel purification kit
ICS 07.080
G66
National Standards of People's Republic of China
Agarose gel recovery kit for determination of general rules
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized.
This standard drafting unit. China Institute of Tested Technology Institute of Biology, China Institute of Test Technology, Kaijie Enterprise Management (Shanghai) has
Limited company, Sichuan Zhongbian Biotechnology Co., Ltd.
The main drafters of this standard. Zhou Lihua, Shi Xiefei, Ma Li Xia, Tang Xiaobo, Hou Xiaoni, Wang Zhi, Li Huai Ping, Ye Deping, Sun Dengfeng,
Tan peace.
Agarose gel recovery kit for determination of general rules
1 Scope
This standard specifies the column agarose gel recovery kit DNA recovery product evaluation indicators and test methods.
This standard applies to column agarose gel recovery kit determination.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB/T 11165 Laboratory pH meter
GB/T 26813 dual-beam UV-visible spectrophotometer
JJG646 pipette test procedures
YY/T 0087 electrophoresis device
YY/T 0657 medical centrifuge
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
DNA purification DNApurification
Using appropriate methods to reduce observable or measurable processes of ligation, cleavage, labeling, amplification and other inhibitors, to obtain higher
Purity DNA.
3.2
DNA recovery DNArecoveryrate
That is, the recovery rate of DNA, the percentage of yield after DNA target fragment recovery and before recovery.
3.3
The maximum sample volume columnreservoircapacity
A single adsorption column of a single maximum loading volume.
3.4
Loading capacity of adsorption column
A single adsorption column can absorb up to the amount of DNA each time. It determines the maximum amount of DNA recovered in a single shot.
4 principle
Media adsorption is a commonly used method of purifying nucleic acids. Column Recycling Kit is a silica gel membrane or other adsorption medium fixed in the distance
As the adsorbent on the column of heart, under the condition of high salt (such as 3.0mol/L ~ 5.0mol/L guanidine hydrochloride) low pH (5.0mol/L ~ 6.5mol/L)
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