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GB/T 29764-2013: Chemicals -- Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 29764-2013 | English | 369 |
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Chemicals -- Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test
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GB/T 29764-2013
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Basic data | Standard ID | GB/T 29764-2013 (GB/T29764-2013) | | Description (Translated English) | Chemicals -- Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 11.100 | | Word Count Estimation | 16,186 | | Quoted Standard | GB/T 27861 | | Regulation (derived from) | National Standards Bulletin No. 18 of 2013 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies: medaka fish (d-rR strain medaka, hereinafter referred to as "fish") early life stage toxicity testing of chemicals the method outlined, equipment, test preparation, test procedures, quality assurance and quality control, data and | GB/T 29764-2013: Chemicals -- Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Fish (Oryzias latipes, d-rR Medaka) early life stage toxicity test
ICS 13.300; 11.100
A80
National Standards of People's Republic of China
Chemicals medaka fish early life stage toxicity test
Issued on. 2013-09-18
2014-03-20 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Table of Contents
Introduction Ⅲ
1 Scope 1
2 Normative references 1
3 Terms and definitions
1 4 test substance information
5 Summary of Method 2
6 Equipment 2
7 Test Preparation 2
8 Test Procedure 3
9 Quality Assurance and Quality Control 6
10 Data and Reports 6
Appendix A (informative) medaka (Oryziaslatipes, d-rRMedaka) domestication and breeding and nurturing environment parameters 8
Annex B (informative) qualified diluted chemical characteristics of water 10
Formulating ingredients in Appendix C (informative) hatching fluid of 11
Reference 12
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard reference to the Organisation for Economic Co-operation and Development (OECD) guidelines for testing of chemicals No.210 (1992 Nian) "fish early life stages
Toxicity Test "(in English), and adds the following main elements.
--- Test biological selection d-rR strain medaka, increased feeding times d-rR strain medaka newly hatched fry, juveniles and parents, investment
Feeding Amount and other information;
--- Anomalies adds multiple embryos and larvae damage deformity statements;
--- An increase in the sex ratio of male and female forms distinguish statements;
--- Statistical parameters increased at the end of the test the sex ratio of male and female fish.
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard was drafted. Research Center for Eco-Environmental Sciences, Chinese Academy of Inspection and Quarantine, the Ministry of Environmental Protection Chemicals Gordon
Hutchison Center, Shanghai testing center.
The main drafters of this standard. Chagin Miao, Wang Zijian, Li Wei, Zhu Lifei, Lizhao Li, Li Xi, Cheng Yan, Liu Chun new.
Chemicals medaka fish early life stage toxicity test
1 Scope
This standard specifies the medaka fish (d-rR strain medaka, hereinafter referred to as "fish") early life stage toxicity test chemical methods overview
Equipment, test preparation, test procedures, quality assurance and quality control, data and reports.
This standard applies to testing the toxicity of chemicals to fish early life stages (embryonic development and juvenile growth phase).
2 Normative references
The following documents for the application of this document is essential, dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 27861 Fish acute toxicity of chemicals
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Lowest observed effect concentration lowestobservedeffectconcentration; LOEC
Compared with the control group, the test substance to produce a significant effect (p < 0.05) the lowest test concentration.
3.2
No observed effect concentration noobservedeffectconcentration; NOEC
Directly below the test of the test substance concentration LOEC.
3.3
Median lethal concentration medianlethalconcentration; LC50
Test concentration causing 50% of the test fish died, expressed in LC50.
4 test substance information
a) structural formula;
b) purity;
c) solubility in water;
d) vapor pressure;
e) hydrolysis of the dissociation constant (pKa);
f) n-octanol/water partition coefficient (Pow);
g) stability in water and light in;
h) in the test solution and reliable quantitative analysis of the test substance and accuracy, detection limit;
i) stability under test conditions;
j) d-rR strain medaka LC50 values;
k) biodegradability test results.
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