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GB/T 29578-2013: Detection and identification of Xanthomonas albilineans (Ashby) Dowson
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| GB/T 29578-2013 | English | 229 |
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Detection and identification of Xanthomonas albilineans (Ashby) Dowson
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GB/T 29578-2013
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Basic data | Standard ID | GB/T 29578-2013 (GB/T29578-2013) | | Description (Translated English) | Detection and identification of Xanthomonas albilineans (Ashby) Dowson | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 10,196 | | Quoted Standard | GB/T 4789.28; SN/T 1193; SN/T 2122 | | Regulation (derived from) | National Standards Bulletin 2013 No. 10 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method of quarantine and identification of bacteria white striped cane. This standard applies to quarantine and identification cane cane seedlings, sugar cane stalks and other plant material in white stripes disease. | GB/T 29578-2013: Detection and identification of Xanthomonas albilineans (Ashby) Dowson---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xanthomonas albilineans (Ashby) Dowson
ICS 65.020.01
B16
National Standards of People's Republic of China
White striped cane quarantine and identification of bacteria
Issued on. 2013-07-19
2013-12-06 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine, Chongqing University.
The main drafters of this standard. Liu Peng, Liuyue Ting, Cui Tiejun, Wang Zhongkang, Liu Fang, Zhang Yujun, Feng Jie, Huang Guoming.
White striped cane quarantine and identification of bacteria
1 Scope
This standard specifies the method for inspection and identification of bacteria cane white stripes.
This standard applies to quarantine and identification of sugarcane cane seedlings, stems and other plant material sugarcane white stripe disease.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 4789.28 Microbiological examination of food hygiene staining, media and reagents
SN/T 1193 genetic testing laboratory technical requirements
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 white striped cane germs basic information
Chinese name. white cane stripes germs.
Scientific name. Xanthomonasalbilineans (Ashby) Dowson.
Disease English. sugarcaneleafscalddisease.
Spp community Bacteria, Proteobacteria door Proteobacteria, γ- Proteobacteria Gang Gammaproteobacteria, Xanthomonas Head
Xanthomonodales, Xanthomonas Branch Xanthomonodaceae, Xanthomonas Xanthomonas.
Mainly by a sense of strain and mechanical dissemination; infected sugarcane is the main mode of transmission of the disease, cutting knives (including loading mechanized cutter) is
An important source of transmission. Clip storm, rodents and beetles, grasshoppers, leafhoppers and sugar cane borer and other pests is also a white stripe disease
way for spreading.
Other basic information, see Appendix A. germs cane white stripes
4 principle of the method
Disease symptoms based on characteristics of sugarcane white stripes on the host plants causing bacteria, and biological characteristics of the pathogen, physiological and biochemical
Characteristics, biological characteristics and molecular characteristics of pathogenic detection and identification.
5 Apparatus appliances
Electronic balance (inductance 0.0001g), incubator, temperature oscillation incubator, autoclave, clean benches, biological microscope,
High-speed refrigerated centrifuge (12000r/min), handheld centrifuge, water meter, water bath pot, BIOLOG bacterial automatic identification system, bacteria
Micro biochemical identification tube cryogenic refrigerator, PCR instrument, electrophoresis, gel imager, vacuum freeze-drying machine, dish (diameter 90mm), adjustable
Type micro-injector (10μL, 100μL, 200μL, 1000μL,), mortar tubes (1.5mL), PCR tubes (0.2μL), a one-time injection
Syringe (2mL) and the like.
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