GB/T 22332: Evolution and historical versions
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Diagnostic techniques for duck virus enteritis
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GB/T 22332-2025
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| GB/T 22332-2008 | English | 149 |
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Diagnostic techniques for duck virus enteritis
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Basic data | Standard ID | GB/T 22332-2025 (GB/T22332-2025) | | Description (Translated English) | Diagnostic techniques for duck virus enteritis | | Sector / Industry | National Standard (Recommended) | | Date of Implementation | 2026-03-01 | | Older Standard (superseded by this standard) | GB/T 22332-2008 | GB/T 22332-2008: Diagnostic techniques for duck virus enteritis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for duck virus enteritis
ICS 11.220
B41
National Standards of People's Republic of China
Duck viral enteritis diagnostic techniques
Posted 2008-08-22
2008-12-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This technology uses a standard part of OIE "Terrestrial animal diagnostic tests and vaccines Handbook" (2004) Recommended test methods, and the specific operation
Program to be refined.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee on Animal Epidemic Prevention.
This standard was drafted. South China Agricultural University, People's Republic of China Guangdong CIQ.
The main drafters of this standard. Guo Xiao Feng, Liao Ming, Hong Jie heart.
Duck viral enteritis diagnostic techniques
1 Scope
This standard specifies the (PCR) diagnostic technique test duck enteritis virus (DVEV) and the isolation and identification of the polymerase chain reaction
Claim.
This standard applies to the diagnosis of duck viral enteritis.
2 clinical symptoms and pathological changes
Domesticated ducks and ducklings from 7 days of age to adulthood can be infected with the disease. In susceptible ducks, initial symptoms is usually sudden persistent high death
Phenomenon, egg production decreased significantly. Due to the different infections of avian species, age, gender, and the virulence of the virus, the outbreak of duck viral enteritis
(DVE) after clinical symptoms and pathological lesions is very different. Duck clinical symptoms of tearing, photophobia, thirst, lack of appetite, ataxia,
Watery diarrhea and nasal discharges. Disease usually duck feather disheveled, anus sticky dirt. With duck wings disease support in order to maintain balance, the entire imaginary appearance
Weak, depression. But two weeks to 7 weeks of age Duck Duck is lower than a loss, the symptoms of dehydration, weight loss, blue beak, anal bloody.
Necropsy found dead duck is not thin. Sexually mature drake's penis may prolapse. Sexually mature female birds ovarian follicles bleeding.
Gross lesions characteristic vascular injury, bleeding tissue with blood and body cavity free, gastrointestinal mucosal surface of the annular-like bleeding and loss of diphtheria
Bad, lymphoid organ damage, organ parenchyma was characterized by degeneration. White Beijing duck characteristic lesions of blood vessels and internal organs damage
Injury, gastrointestinal epithelial cells showed nuclear eosinophilic inclusion bodies and cytoplasmic inclusion bodies.
3 virus isolation
3.1 Material preparation
3.1.1 diseased collection. general should be taken from live birds or poultry dying in the early stages of the infection or the onset of the acute phase. Collection of birds dying of liver, spleen, brain
And other tissue samples. Live bird with a sterile cotton swab smear cloaca. Cotton swab with secretions into Penicillium 1000IU per milliliter
Su, 1000 ug streptomycin, phosphate buffer of pH7.2 ~ 7.6 (PBS) medium. Diseased submission should be placed 50% of Gan Yousheng Li salt
Water.
Save 3.1.2 Diseased. The collected samples if processed within 48h, can be stored at 4 ℃; otherwise, the following should be placed at -20 ℃ (-70 ℃
Storage is best).
3.1.3 Disease material handling. cotton swab full twisting action to remove the swab wring. Samples were dried 3000r/min4 ℃ centrifugation 30min, take
Supernatant as inoculum material. Tissue samples first with PBS, pH7.2 ~ 7.6 made 5 to 10 times emulsion, 3000r/min4 ℃ centrifugation
30min, the supernatant as inoculum material. To avoid bacterial contamination, may be added in a liquid sample penicillin (1000IU/mL), streptomycin
(1000μg/mL), kanamycin (1000μg/mL), 37 ℃ incubator role 30min. Sterility testing.
3.1.4 duck embryo. 10 days to 11 days of non-DVE vaccine duck embryos.
3.2 experimental operation
3.2.1 embryo inoculation. learn to handle and inspection sterile sample, an amount of 0.2mL/embryo chorioallantoic membrane inoculation by 10 ...
Non-DVE vaccine in 11-day-old duck embryo, each sample was inoculated with four to five embryos were incubated at 38 ℃ ~ 38.5 ℃ incubator.
72h 1 day before the embryo according to 2 times, according to the embryo after five times a day. 72h before death discarded embryos, frozen embryos die within 72h ~ 120h
Or viable embryos.
3.2.2 viral harvest. a sterile collect dead embryos or embryos 72h ~ 120h live within the chorioallantoic membrane and allantoic fluid, -20 ℃ stored for use.
3.2.3 As the first generation of isolated negative results, to be blind pass three generations.
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