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GB/T 21854-2025: Chemicals - Fish early-life stage toxicity test
Status: Valid GB/T 21854: Evolution and historical versions
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| GB/T 21854-2025 | English | 439 |
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Chemicals - Fish early-life stage toxicity test
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GB/T 21854-2025
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| GB/T 21854-2008 | English | 399 |
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Testing of chemicals -- Fish, early-life stage toxicity test
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GB/T 21854-2008
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Basic data | Standard ID | GB/T 21854-2025 (GB/T21854-2025) | | Description (Translated English) | Chemicals - Fish early-life stage toxicity test | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300 | | Word Count Estimation | 22,299 | | Date of Issue | 2025-08-29 | | Date of Implementation | 2025-12-01 | | Older Standard (superseded by this standard) | GB/T 21854-2008 | | Issuing agency(ies) | State Administration for Market Regulation, National Standardization Administration | GB/T 21854-2025: Chemicals - Fish early-life stage toxicity test---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT21854-2025
ICS 13.300
CCSA80
National Standard of the People's Republic of China
Replaces GB/T 21854-2008
Chemical Toxicity Test for Fish Early Life Stages
Released on August 29, 2025
Implementation on December 1, 2025
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface III
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Test substance information 2
5 Test Principle 2
6 Instruments and Equipment 2
7 Experimental Preparation 3
8 Test Procedure 3
9 Quality Assurance and Quality Control 5
10 Data and Reports 6
Appendix A (Informative) Test conditions, cycles and survival rates of recommended fish species 8
Appendix B (Informative) Recommended Feeding and Reproduction Requirements for Test Fish Species 9
Appendix C (Informative) Chemical properties of qualified dilution water 10
Appendix D (Informative) Statistics of NOEC determinations based on different endpoints 11
Appendix E (Informative) Statistics of Regression Estimation 14
Reference 16
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 21854-2008 “Chemicals - Toxicity to Early Life Stages of Fish” and is consistent with GB/T 21854-2008.
In addition to structural adjustments and editorial changes, the main technical changes are as follows.
--- Added the terms and definitions of "harpoon length", "standard length of fish", "full length of fish", "effect concentration" and "unknown complex substance" (see 3.1~3.4,
3.7);
--- Changed the test substance information content (see Chapter 4, Chapter 3 of the.2008 edition);
--- Changed the test principle (see Chapter 5, 4.1 of the.2008 edition);
--- Changed the instrumentation (see Chapter 6, Chapter 5 of the.2008 edition);
--- Changed the test preparation and test procedures (see Chapters 7 and 8, Chapters 6 and 7 of the.2008 edition);
--- Modified quality assurance and quality control (see Chapter 9, Chapter 8 of the.2008 edition);
--- Changed data and reporting (see Chapter 10, Chapter 9 of the.2008 edition).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Technical Committee for Standardization of Hazardous Chemicals Management (SAC/TC251).
This document was drafted by. Solid Waste and Chemicals Management Technology Center of the Ministry of Ecology and Environment, Liaoning Provincial Ecological Environment Affairs Service Center,
Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Shanghai Testing Center, Beijing Normal University, Shanghai Academy of Environmental Sciences, Guangdong Academy of Sciences
Institute of Microbiology (Guangdong Provincial Microbiological Analysis and Testing Center).
The main drafters of this document are. Liu Xiaojian, Liu Hongying, Teng Xiaoming, Bai Yang, Wang Lei, Ma Yan, Yang Li, Yang Kun, Dong Mengqi, Li Wenchao, Yang Li,
Li Feifan, Chen Xiaoqian, Liu Jining, Hu Shuangqing, Chen Guilan, Yang Xichen, Zhang Hongchang, Hu Junjie, and Lu Ling.
The previous versions of this document and the documents it replaces are as follows.
---First published in.2008 as GB/T 21854-2008;
---This is the first revision.
Chemical Toxicity Test for Fish Early Life Stages
1 Scope
This document specifies the test substance information, instrumentation, test system, test procedures, quality control, and other related information for the early life stage toxicity test of chemicals in fish.
Quality assurance and quality control, data and reporting requirements.
This document is applicable to the testing of chemicals for lethal and sublethal effects on early life stages of fish to evaluate chronic toxicity and
Subchronic toxic effects.
2 Normative references
This document has no normative references.
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
fishforklength;FFL
The straight-line distance from the tip of the snout to the end of the mid-tail fin line is used to measure the body length of fish in which the end of the vertebral column is difficult to distinguish.
3.2
fish standard length fishstandardlength;FSL
The straight-line distance from the tip of the snout to the end of the last vertebra at the base of the caudal fin or to the base of the caudal fin, excluding the length of the caudal fin.
3.3
Fish total length; FTL
The straight-line distance from the tip of the snout to the end of the longer lobe of the caudal fin.
3.4
effect concentration;ECx
The concentration of the test substance that causes a certain observed effect in x% of the test organisms compared with the control organisms during a given test period.
[Source. HJ1257-2022, 2.1.33]
3.5
The lowest concentration of a substance that produces a statistically significant effect (p less than 0.05) on the test organism compared with the control group within a given test period
Test substance concentration.
[Source. HJ1257-2022, February 1, 38]
3.6
No statistically significant effect (p greater than or equal to 0.000) was observed on the test organisms compared to the control group within a given test period.
0.05) of the highest test substance concentration.
[Source. HJ1257-2022, February 1, 39]
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