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GB/T 19495.5-2018: Detection of genetically modified organisms and derived products -- Quantitative real-time polymerase chain reaction (PCR) methods
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| GB/T 19495.5-2018 | English | 439 |
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Detection of genetically modified organisms and derived products -- Quantitative real-time polymerase chain reaction (PCR) methods
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GB/T 19495.5-2018
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| GB/T 19495.5-2004 | English | RFQ |
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Detection of genetically modified organisms and derived products -- Quantitative nucleic acid-based methods
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GB/T 19495.5-2004
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Basic data | Standard ID | GB/T 19495.5-2018 (GB/T19495.5-2018) | | Description (Translated English) | Detection of genetically modified organisms and derived products -- Quantitative real-time polymerase chain reaction (PCR) methods | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 22,262 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Older Standard (superseded by this standard) | GB/T 19495.5-2004 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 19495.5-2018: Detection of genetically modified organisms and derived products -- Quantitative real-time polymerase chain reaction (PCR) methods
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Detection of genetically modified organisms and derived products - Quantitative real-time polymerase chain reaction (PCR) methods
ICS 65.020.01
B16
National Standards of People's Republic of China
Replace GB/T 19495.5-2004
Genetically modified product testing
Real-time fluorescence quantitative polymerase chain reaction (PCR)
Detection method
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
GB/T 19495 "GMO Testing" is divided into the following sections.
---GB/T 19495.1 General requirements and definitions for the detection of genetically modified products;
---GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products;
---GB/T 19495.3 Detection of nucleic acid extraction and purification methods for transgenic products;
---GB/T 19495.4 Transgenic product detection real-time fluorescence qualitative polymerase chain reaction (PCR) detection method;
---GB/T 19495.5 Transgenic product detection real-time fluorescence quantitative polymerase chain reaction (PCR) detection method;
---GB/T 19495.6 Genetically modified product detection gene chip detection method;
---GB/T 19495.7 GM product testing sampling and sample preparation methods;
---GB/T 19495.8 Detection method for protein detection of genetically modified products;
---GB/T 19495.9 Detection method of liquid product chip for detection of plant products by genetically modified products.
This part is the fifth part of GB/T 19495.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
This part replaces GB/T 19495.5-2004 "Quantitative PCR detection method for detection of nucleic acid in genetically modified products". And GB/T 19495.5-
Compared with.2004, the main technical changes except editorial changes are as follows.
--- Revised the scope of application of the standard;
--- Added the ability to quantitatively detect transgenic plant lines in samples based on matrix reference materials and plasmid standard molecules, respectively.
Procedure
--- Increased the copy number of transgenic plant lines such as soybean, corn, canola, cotton, rice, potato, sugar beet and papaya
Quantitative detection method.
This part is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This section drafted by. China Academy of Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people
Heguo Shandong Entry-Exit Inspection and Quarantine Bureau and Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Huang Xin, Li Xiang, Gao Hongwei, Ling Xingyuan, Zhu Shuifang, Chen Hongjun, Pan Liangwen, Cao Jijuan, Zhang Guiming.
The previous versions of the standards replaced by this section are.
---GB/T 19495.5-2004.
Genetically modified product testing
Real-time fluorescence quantitative polymerase chain reaction (PCR)
Detection method
1 Scope
This part of GB/T 19495 specifies soybeans, corn, rapeseed, rice (rice), cotton, potatoes, beets, papaya and other plants and
A real-time fluorescent PCR quantitative detection method for the content of transgenic lines in products.
This section applies to quantitative detection methods for hundreds of copies of transgenic lines in the above plants and their products.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products
GB/T 19495.3 Transgenic product detection nucleic acid extraction and purification method
GB/T 19495.7 GM product testing sampling and sample preparation method
GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing
JJF1059.1 Measurement Uncertainty Evaluation and Representation
SN/T 4562 Transgenic Testing Laboratory Measurement Uncertainty Evaluation Guide
3 Terms, definitions and abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Transgenic transgene
Functional DNA sequences derived from other species that are not native to the species, through bioengineering techniques, allowing them to enter the species
Lines are expressed in order to give the species the technology to acquire new traits.
3.1.2
Line-specific event-specific
The contiguous region sequence resulting from the recombination of the foreign DNA into the recipient crop genome.
3.1.3
Real-time fluorescence PCR real-timepolymerasechainreaction
Adding a fluorophore to the polymerase chain reaction system, monitoring the entire PCR process in real time using fluorescence signal accumulation, and passing the standard
A method of quantitative analysis of an unknown template by a curve.
3.1.4
Internal standard gene endogenousreferencegene
A gene with a constant copy number and no display of allelic changes in the species is detected. This gene can be used to determine species specificity.
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