|
US$439.00 ยท In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 18637-2018: Technical specifications for diagnosis of bovine viral diarrhea/mucosal disease
Status: Valid GB/T 18637: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 18637-2018 | English | 439 |
Add to Cart
|
4 days [Need to translate]
|
Technical specifications for diagnosis of bovine viral diarrhea/mucosal disease
| Valid |
GB/T 18637-2018
|
| GB/T 18637-2002 | English | 359 |
Add to Cart
|
3 days [Need to translate]
|
Diagnostic technique for bovine viral diarrhoea/mucosal disease
| Obsolete |
GB/T 18637-2002
|
PDF similar to GB/T 18637-2018
Basic data | Standard ID | GB/T 18637-2018 (GB/T18637-2018) | | Description (Translated English) | Technical specifications for diagnosis of bovine viral diarrhea/mucosal disease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 22,271 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Older Standard (superseded by this standard) | GB/T 18637-2002 | | Regulation (derived from) | National Standard Announcement No. 11 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 18637-2018: Technical specifications for diagnosis of bovine viral diarrhea/mucosal disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical specifications for diagnosis of bovine viral diarrhea/mucosal disease
ICS 11.220
B41
National Standards of People's Republic of China
Replace GB/T 18637-2002
Technical specification for diagnosis of bovine viral diarrhea/mucosal disease
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces GB/T 18637-2002 "Blood virus diarrhea/mucosal disease diagnosis technology", in addition to editorial changes, the main technical changes
The following is as follows.
--- Added "clinical diagnosis" method (see Chapter 4);
--- Added "real-time fluorescent RT-PCR detection" (see 5.4).
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
This standard was drafted. China Veterinary Drug Inspection Institute, Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Yangzhou University, Chinese
Ningxia Entry-Exit Inspection and Quarantine Bureau of the Republic of China, Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Animal Health and Epidemiology
center.
The main drafters of this standard. Li Junping, Liu Huan, Yin Chunsheng, Tao Jie, Hao Junhu, Pu Jing, Fan Xuezheng, Wu Huawei, Sun Wei, Wang Jianhua,
Song Jiande, Zhu Guoqiang.
The previous versions of the standards replaced by this standard are.
---GB/T 18637-2002.
Introduction
Bovine Viral Diarrhea/Mucosal Disease (BVD/MD, BVD for short) is caused by bovine disease
Toxic diarrhea/mucosal disease virus (BovineViralDiarrhea/Mucosal Disease Virus, BVD/MDV, referred to as BVDV) infection
Bovine-induced fever, mucosal erosion ulcers, leukopenia, diarrhea, cough, and miscarriage of pregnant cows or the production of malformed fetuses
An infectious disease. BVDV is a taxonomic genus of the genus Flaviviridae and Pestivirus.
The species, currently identified by the International Classification of Viruses, BVDV has two genotypes (or species), namely BVDV-1 and BVDV-2. it
They are associated with the same family of swine fever virus (ClassicalSwineFeverVirus, CSFV) and Border Disease Virus (BorderDiseaseVirus,
There is a structural and antigenic association between BDV) and a partial cross-reaction in serology. The disease caused by BVDV-1 is globally distributed.
More common, and the BVDV-2 caused by the disease is rare. Both genotypes of virus can produce cells based on their cultured cells.
The lesion is divided into two biotypes, Cytopathic Type (CP) and Non-cytopathic (Non-cytopathic).
Type, NCP). NCP strains are biotypes that cause all types of infections and pathologies, while CP strains are mainly found in mucosal cases.
At present, the disease is widespread in the world. In 1983, China first isolated BVDV. In recent years, pathogen surveys have shown
China's cattle, sheep, deer, pigs, etc. are infected with BVDV to varying degrees, which has seriously affected the healthy development of animal husbandry in China.
The methods described in this standard are commonly used in the diagnosis of BVD in China in recent years, including clinical diagnosis and pathological diagnosis.
Broken and serological diagnostic techniques. Serological diagnostic techniques are virus neutralization assays. Pathogenic diagnostic techniques include virus isolation and identification and real-time fluorescence
Real RT-PCR (Real-time RT-PCR) detection method, the Real-time RT-PCR detection method in this standard has BVDV specificity
Sexuality, capable of simultaneously detecting BVDV-1 and BVDV-2, and specifically distinguishing between BVDV and CSFV.
Combined with China's animal epidemic prevention laws, regulations and policies, this standard can effectively diagnose and monitor the occurrence of BVD/MD in China.
popular.
Technical specification for diagnosis of bovine viral diarrhea/mucosal disease
1 Scope
This standard specifies the clinical diagnosis of epidemiology, clinical symptoms, pathological changes of bovine viral diarrhea/mucosal disease, and sample collection and
Technical requirements and operational specifications for laboratory diagnostics such as isolation, identification and identification, Real-time RT-PCR and virus neutralization tests.
This standard is applicable to the detection of type 1 and type 2 bovine viral diarrhea/mucosal disease virus, nucleic acid and antibody levels in different samples.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 19438.1-2004 Avian influenza virus universal fluorescent RT-PCR detection method
GB 19489 General requirements for laboratory biosafety
GB/T 27401 Laboratory Quality Control Regulations Animal Quarantine
3 Abbreviations
The following abbreviations apply to this document.
BT. Bovine Turbinatecel
BVD/MD. Bovine Viral Diarrhea/Mucosal Disease
BVD/MDV. Bovine Viral Diarrhea/Mucosal Disease Virus
CPE. Cytopathic Effect (CytopathicEffect)
CP. Cytopathic type
cRNA. Complement RNA (ComplementRNA)
Ct(Cp). the number of cycles experienced by the amount of fluorescent signal in each reaction tube reaching a set threshold (CycleThreshold, or
CrossingPoint)
DEPC. DiethylPyrocarbonate
FAM. Carboxyfluorescein
FBS. fetal bovine serum (FetalBovineSerum)
FITC. Fluorescein Isothiocyanate
MDBK. Bovine kidney secondary cells (Madin-DarbyBovineKidneycel)
MEM. Basic Medium (MinimumEssentialMedium)
NCP. Non-cytopathic type
PBS. Phosphate Buffer (PhosphateBufferSaline)
PBST. Tween Phosphate Buffer (PhosphateBufferSolutionwithTween20)
PI. Persistent Infection
Real-time RT-PCR. Real-time Reverse Transcription Polymerase Chain Reaction (Real-time Reverse Transcription Polymerase
ChainReaction)
|