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GB/T 18204.4-2025: Examination methods for public places - Part 4: Microorganism indicators on a surface of public articles
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Examination methods for public places - Part 4: Microorganism indicators on a surface of public articles
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GB/T 18204.4-2025
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| GB/T 18204.4-2013 | English | 275 |
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Examination methods for public places - Part 4: Microorganism on a surface of public articles
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| GB/T 18204.4-2000 | English | 199 |
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Methods of microbiological examination for towel and bedclothes in public places. Determination of aerobic bacterial count
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GB/T 18204.4-2000
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Basic data | Standard ID | GB/T 18204.4-2025 (GB/T18204.4-2025) | | Description (Translated English) | Examination methods for public places - Part 4: Microorganism indicators on a surface of public articles | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C51 | | Classification of International Standard | 13.060 | | Word Count Estimation | 26,257 | | Date of Issue | 2025-05-30 | | Date of Implementation | 2025-12-01 | | Older Standard (superseded by this standard) | GB/T 18204.4-2013 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 18204.4-2025: Examination methods for public places - Part 4: Microorganism indicators on a surface of public articles
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ICS 13.060
CCSC51
National Standard of the People's Republic of China
Replaces GB/T 18204.4-2013
Public Place Hygiene Inspection Methods
Part 4.Microbiological indicators for public supplies and utensils
Released on 2025-05-30
2025-12-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of contents
Preface III
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Total bacteria count 2
5 Coliform bacteria 4
6 Staphylococcus aureus 7
7 Total fungi 10
8 β-hemolytic Streptococcus 12
Appendix A (Normative) Microbiological sampling methods for public supplies and utensils in public places 15
Appendix B (Normative) Quality Control and Biosafety 17
Reference 18
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document is part 4 of GB/T 18204 “Test methods for hygiene in public places”. GB/T 18204 has been published in the following parts.
--- Part 1.Physical properties;
--- Part 2.Chemical properties;
--- Part 3.Air microbial indicators;
--- Part 4.Microbiological indicators for public supplies and utensils;
--- Part 5.Central air conditioning and ventilation systems;
--- Part 6.Technical specifications for health monitoring.
This document replaces GB/T 18204.4-2013 "Public Place Hygiene Inspection Methods Part 4.Microorganisms of Public Supplies and Utensils" and
Compared with GB/T 18204.4-2013, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The terms and definitions have been changed (see Chapter 3, Chapter 2 of the.2013 edition);
b) Some instruments and consumables have been changed (see 4.2, 5.2, 6.2, 7.2 and 8.2, 3.2, 4.2, 5.2, 6.2 and 7.2 of the.2013 edition);
c) Some culture media and reagents have been changed (see 6.3 and 8.3, 5.1 and 7.1 of the.2013 edition);
d) The inspection steps have been changed (see 4.4, 5.4, 6.4, 7.4 and 8.4, 3.3, 4.3, 5.3, 6.3 and 7.3 of the.2013 edition);
e) Added biochemical identification methods such as the catalase test for β-hemolytic streptococci (see 8.4, 7.3 of the.2013 edition);
f) The calculation rules and result reports have been changed (see 4.6, 4.7, 5.5, 6.5, 7.6, 7.7 and 8.5, 3.5, 3.6, 4.4, 5.4,
6.4, 6.5 and 7.4);
g) The microbial sampling method for public supplies and utensils in public places has been changed (see Appendix A, Appendix A of the.2013 edition);
h) Increased quality control and biosafety (see Appendix B).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Center for Disease Control and Prevention.
This document was drafted by. Jiangsu Provincial Center for Disease Control and Prevention, Changzhou Center for Disease Control and Prevention, Nanjing Center for Disease Control and Prevention,
Suzhou Center for Disease Control and Prevention, Southeast University, Institute of Environmental and Health-Related Product Safety, Chinese Center for Disease Control and Prevention.
The main drafters of this document are. Ding Zhen, Ma Kai, Ma Xiaoying, Wang Fengming, Yong Wei, Shen Qiang, Qian Hua, Zhou Zhirong, Zhang Fugang, Zhou Lian and Zhao Ying.
The previous versions of this document and the documents it replaces are as follows.
---GB/T 17220-1998 "Technical Specifications for Hygiene Monitoring in Public Places" was first published in.1998 and in.2000
GB/T 18204.2-2000 "Microbiological examination method for tea sets in public places - Determination of total bacterial count", GB/T 18204.3-
2000 "Microbiological Examination Methods for Teaware in Public Places - Determination of Coliform Groups", GB/T 18204.4-2000 "Towels,
GB/T 18204.5-2000 Microbiological examination methods for bedding and pillowcases - Determination of total bacterial count, GB/T 18204.5-2000 Towels, bedding and pillowcases in public places
GB/T 18204.6-2000 Microbiological examination method for coliform bacteria in hairdressing utensils
GB/T 18204.7-2000 "Microbiological test method for hairdressing utensils - Determination of Staphylococcus aureus",
GB/T 18204.8-2000 "Microbiological test methods for slippers in public places - Determination of mold and yeast", GB/T 18204.11-
2000 "Microbiological examination methods for bathtubs, washbasins (foot basins) in public places - Determination of total bacterial count", GB/T 18204.12-2000 "Microbiological examination methods for bathtubs, washbasins (foot basins) in public places - Determination of total bacterial count"
Microbiological inspection methods for bathtubs, face (foot) basins in places - Determination of coliform bacteria";
---First revised in.2013 as GB/T 18204.4-2013, replacing GB/T 18204.2~18204.8-2000,
GB/T 18204.11~18204.12-2000, partially replaces GB/T 17220-1998;
---This is the second revision.
Introduction
GB/T 18204 "Public Place Hygiene Inspection Methods" is a national recommended standard and is consistent with GB 37488 "Public Place Hygiene Indicators and Limits".
GB/T 18204 (all parts) is used in conjunction with the "Requirements for Public Places" and is the recommended standard test method for public place hygiene monitoring.
The publication is conducive to standardizing sample collection, on-site and laboratory testing in public places, and improving the precision and accuracy of test results of testing institutions.
This document is part 4 of GB/T 18204.
This revision improves the test methods for microbiological indicators of public supplies and utensils in GB 37488 and supplements the results of various indicators.
The biochemical identification step of β-hemolytic streptococcus was added to further improve the advancement, operability and
Applicability, meeting the needs of health inspection in public places.
GB/T 18204 consists of 6 parts.
--- Part 1.Physical indicators. The purpose is to provide the basic principles and
Require.
--- Part 2.Chemical indicators. The purpose is to provide sample collection and laboratory testing of chemical indicators in public places.
Basic principles and requirements.
--- Part 3.Air microbial indicators. The purpose is to provide sample collection and laboratory testing of air microbial indicators in public places.
Basic principles and requirements of testing.
--- Part 4.Microbiological indicators of public supplies and utensils. The purpose is to provide microbiological indicators of public supplies and utensils in public places.
Basic principles and requirements for sample collection and laboratory testing.
--- Part 5.Central air conditioning and ventilation systems. The purpose is to provide sample collection of indicators of central air conditioning and ventilation systems in public places
and basic principles and requirements of laboratory testing.
--- Part 6.Technical specifications for health monitoring. The purpose is to standardize the frequency and sample size of health monitoring in public places, quality control, etc.
Technical requirements.
Public Place Hygiene Inspection Methods
Part 4.Microbiological indicators for public supplies and utensils
1 Scope
This document describes the total number of bacteria, fungi, coliform bacteria, Staphylococcus aureus and β-lysine on the surfaces of public utensils in public places.
Sampling and testing methods for Streptococcus sanguinis.
This document is applicable to the inspection of microbial indicators of public supplies and utensils in public places, and other places shall refer to it.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB 4789.28 National food safety standard Quality requirements for culture media and reagents for food microbiology testing
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB 19489 General requirements for laboratory biosafety
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Total bacterial count
The growth of mesophilic aerobic and facultative anaerobic bacterial colonies on nutrient agar medium at 36℃±1℃ for 48h
total.
3.2
coliforms
Aerobic and facultative anaerobic Gram-negative, non-spore-forming bacillus that can ferment lactose, produce acid and gas under certain culture conditions.
3.3
Staphylococcus aureus
It grows well on Baird-Parker plates, forms a completely transparent hemolytic ring on blood plates, and is Gram-positive in plasma coagulase tests.
Positive cocci are often arranged in a staphylococcal shape.
3.4
Total fungal count
The number of colonies formed on Sabouraud agar medium after cultivation at 28℃±1℃ for 3d~5d.
3.5
A suppurative (or A) infection that produces hemolysin and forms a clear, completely transparent hemolytic ring (β-hemolysis) around the colonies on the blood agar plate.
group) Streptococci and agalactiae (or group B) Streptococci.
[Source. GB 4789.11-2014, 2.2, modified]
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