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GB 17512.2-2010 English PDF

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GB 17512.2-2010: National food safety standards -- Food additives -- Erythrosine aluminum lake
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GB 17512.2: Evolution and historical versions

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GB 17512.2-2010English329 Add to Cart 3 days [Need to translate] National food safety standards -- Food additives -- Erythrosine aluminum lake Valid GB 17512.2-2010
GB 17512.2-1998English359 Add to Cart 3 days [Need to translate] Food additive. Erythrosine aluminum lake Obsolete GB 17512.2-1998

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Basic data

Standard ID GB 17512.2-2010 (GB17512.2-2010)
Description (Translated English) National food safety standards -- Food additives -- Erythrosine aluminum lake
Sector / Industry National Standard
Classification of Chinese Standard X42
Classification of International Standard 67.220.20
Word Count Estimation 14,120
Date of Issue 2010-12-21
Date of Implementation 2011-02-21
Older Standard (superseded by this standard) GB 17512.2-1998
Regulation (derived from) Ministry of Health Bulletin No. 19 of 2010
Issuing agency(ies) Ministry of Health of the People's Republic of China
Summary This Chinese standard applies to food additives and erythrosine and aluminum hydroxide reacts add and erythrosine aluminum lake.

GB 17512.2-2010: National food safety standards -- Food additives -- Erythrosine aluminum lake

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National food safety standards - food additives - erythrosine aluminum lake National Food Safety Standard Food Additives erythrosine aluminum lake Issued on. 2010-12-21 2011-02-21 implementation National Standards of People's Republic of China People's Republic of China Ministry of Health issued

Foreword

This standard replaces GB 17512.2-1998 "food additive erythrosine aluminum lake." This standard compared with GB 17512.2-1998, the main technical changes are as follows. - Added safety tips; - Modify the Test method for identification; - Increase the spectrophotometric colorimetry parallel determination of allowable difference; - Canceled Chloride (NaCl) and sulfate (Na2SO4 to count) indicators; - Arsenic (As) is modified by chemical detection methods limit law atomic absorption spectrometry; - Cancel the heavy metals (as Pb) indicator; - Increase the lead (Pb) indicator and detection methods; - Added control indicators and detection methods of zinc; - Barium (Ba) detection method to modify the barium sulfate precipitation assay limits. Appendix A of this standard is a normative appendix. This standard replaces the standards previously issued as follows. --GB 17512.2-1998. National Food Safety Standard Food Additives erythrosine aluminum lake

1 Scope

This standard applies to food additives produced by the erythrosine and aluminum hydroxide additive effect erythrosine aluminum lake.

2 Normative references

The standard file referenced in the application of this standard is essential. For cited documents with dates, only the date of Version applies to this standard. For undated references, the latest edition (including any amendments) applies to this standard.

3 formula and relative molecular mass

Formula 3.1 C20H6I4Na2O5 · H2O 3.2 relative molecular mass 897.87 (according to 2007 international relative atomic mass) 4. Technical Requirements 4.1 Sensory requirements. comply with Table 1. Table 1 Sensory requirements Project requires test methods Red color By visual assessment of natural light. Organization Status powder 4.2 Physical indicators. to comply with Table 2. Table 2. Physical and chemical indicators Item Index Test Method Erythrosine (sodium salt dollars), w /% ≥ 10.0 Appendix A A.4 Loss on drying, w /% ≤ 30.0 Appendix A A.5 Hydrochloric acid and ammonia water insolubles, w /% ≤ 0.5 A.6 in Appendix A Deputy dye, w /% ≤ 1.5 Appendix A A.7 Sodium iodide, w /% ≤ 0.2 Appendix A A.8 Arsenic (As)/(mg/kg) ≤ 3.0 A.9 in Appendix A Lead (Pb)/(mg/kg) ≤ 10.0 Appendix A A.10 Zinc (Zn)/(mg/kg) ≤ 50.0 A.11 Appendix A Barium (Ba), w /% ≤ 0.05 Appendix A A.12

Appendix A

(Normative) Testing method A.1 Safety Tips Reagents The standard test methods used for toxic or corrosive, according to the relevant provisions of the operation, the operation need to be careful. If splashed on the skin should immediately wash with water, severe cases should be treated immediately. When using a volatile acid, to be carried out in a fume hood. A.2 General Provisions This standard reagents and water, did not indicate when the other requirements, refer to three analytical reagent and GB/T 6682-2008 specified water. Standard test solution required impurity standard solution, preparations and products at the time did not indicate other provisions, according to GB/T 601, GB/T 602, GB/T 603 regulations formulated and calibration. A.3 Identification Test A.3.1 Reagents and materials A.3.1.1 sulfuric acid. A.3.1.2 hydrochloric acid solution. 13. A.3.1.3 sodium hydroxide solution. 90g/L. A.3.1.4 ammonium acetate solution. 1.5g/L. A.3.1.5 activated carbon. A.3.2 Instruments and Equipment A.3.2.1 Spectrophotometer. A.3.2.2 cuvette. 10mm. A.3.3 Identification method It should meet the following conditions. A.3.3.1 Weigh about 0.1g sample, add 5mL of sulfuric acid, at 50 ℃ ~ 60 ℃ water bath shaken from time to time, about 5min when heated, the solution was Orange-red. After cooling, the supernatant take 2 to 3 drops of drops, add 5mL water, the solution was red. A.3.3.2 Weigh about 0.1g sample, add 5mL sodium hydroxide solution was heated and dissolved in a water bath, add ammonium acetate solution equipped to 100mL, Centrifuged the solution is not clarified. Then take this solution 1mL ~ 10mL, plus ammonium acetate solution equipped to 100mL, the measurement of absorbance In the range of 0.2 to 0.7, a maximum absorption wavelength of this solution was 526 nm ± 2nm. A.3.3.3 Weigh about 0.1g sample was added 10mL hydrochloric acid solution and heated in a water bath, so that most of the dissolved. Add 0.5g of activated carbon, charge Shake points after filtration. Take a colorless filtrate, add sodium hydroxide solution and after rendering aluminum reaction. A.4 Determination erythrosine aluminum lake of A.4.1 Method summary After sample processing were dissolved in an aqueous medium or with known content erythrosine standards, diluted with ammonium acetate solution after constant volume, At the maximum absorption wavelength, were measured absorbance value, and then calculate the content. A.4.2 Reagents and materials A.4.2.1 ammonia solution. 13. A.4.2.2 ammonium acetate solution. 1.5g/L. A.4.2.3 erythrosine standard. ≥85.0% (mass fraction, according to GB/T 17512.1-2010 A.4.1 Determination). A.4.3 Instruments and Equipment A.4.3.1 Spectrophotometer. A.4.3.2 cuvette. 10mm. A.4.4 formulated erythrosine standard solution Weigh about 0.25g erythrosine standard (accurate to 0.0001g), was dissolved in an appropriate amount of ammonium acetate solution, transferred to 1000mL volumetric flask, Diluted with water to the mark. Draw 10mL, transferred to 500mL volumetric flask, add ammonium acetate solution was diluted to the mark. A.4.5 formulated erythrosine aluminum lake of the sample solution Weigh about 0.5g sample (accurate to 0.0001g), transferred to 250mL beaker, 150mL ammonia solution, stirring occasionally until the After dissolution transferred 500mL volumetric flask, dilute to the mark, shake. Draw 10mL transferred to 500mL volumetric flask, with ammonium acetate solution It was diluted to the mark. A.4.6 Analysis step Erythrosine standard solution and the sample solution erythritol red aluminum lake were placed in 10mm cuvettes, with the maximum absorption wavelength with points Light photometer respective absorbance values of ammonium acetate solution as reference solution. A.4.7 Calculation Results Erythrosine aluminum lake mass fraction 1w its value is expressed in%, according to formula (A.1) Calculated. 1) 500/10 500/( ) 500/101000/(w mA mAw ×× × = (A.1) Where. A1 - absorbance values Erythrosine aluminum lake of the sample solution; Quality value m0-- erythrosine standards, in units of grams (g); 0w - erythrosine standard mass fraction%; Absorbance values A0-- erythrosine standard solution; Quality value m1-- erythrosine aluminum lake sample in grams (g). The results represent a decimal. The absolute difference between the parallel determination results is not more than 1.0% (mass fraction), the arithmetic mean value as a measurement result. A.5 Determination of loss on drying A.5.1 Analysis step Weigh about 2g sample (accurate to 0.001g), has been placed in the weighing bottle constant at 135 ℃ ± 2 ℃ constant temperature oven, in 135 ℃ ± 2 ℃ constant temperature oven drying to constant weight. A.5.2 Calculation Results Loss on drying mass fraction to 2w and its value is expressed in%, according to formula (A.2) Calculated. 2 × - = m mmw (A.2) Where. Numerical m2-- sample before drying mass in grams (g); m3-- sample dried to a constant mass values, expressed in grams (g). The results represent a decimal. The absolute difference between the parallel determination results is not more than 0.2% (mass fraction), the arithmetic mean value as a measurement result. A.6 Determination of hydrochloric acid and ammonia water insolubles A.6.1 Reagents and materials A.6.1.1 hydrochloric acid. A.6.1.2 hydrochloric acid solution. 37. A.6.1.3 ammonia solution. 496. A.6.1.4 silver nitrate solution. c (AgNO3) = 0.1mol/L. A.6.2 Instruments and Equipment A.6.2.1 sand core glass crucible. G4, a pore size of 5μm ~ 15μm. A.6.2.2 oven thermostat. A.6.3 Analysis step Join Weigh about 2g sample (accurate to 0.001g), placed in 600mL beaker, add 20mL of water and 20mL of hydrochloric acid, stir 300mL hot water, stir well, cover the surface of the dish, heating 30min, cooled at 70 ℃ ~ 80 ℃ water bath, use has been baked at 135 ℃ ± 2 ℃ to constant The amount G4 sintered glass filter crucible, with about 30mL of water to rinse the beaker insolubles to G4 sintered glass crucible to colorless after lotion, Washed first with aqueous ammonia solution 100mL, after washed with 10mL of hydrochloric acid solution, then washed with water until the silver nitrate solution was washed with a non-white test Precipitation, followed by 135 ℃ ± 2 ℃ constant temperature oven drying to constant weight. A.6.4 Calculation Results Hydrochloric acid and ammonia water insolubles mass fraction 3w and its value is expressed in%, according to formula (A.3) Calculated. 3 × = m mw (A.3) Where. m4 - Numerical dried water insoluble mass in grams (g); m5 - the value of the sample mass, in grams (g). The results represent two decimal. The absolute difference between parallel determination results is not more than 0.05% (mass fraction), the arithmetic mean value as a measurement result. Determination A.7 deputy dye A.7.1 Method summary The components are separated by paper chromatography, eluted and quantified by spectrophotometry. A.7.2 Reagents and materials A.7.2.1 ethanol. A.7.2.2 n-butanol. Acetone solution A.7.2.3. 1 1. A.7.2.4 ammonia solution. 496. A.7.2.5 sodium bicarbonate solution. 4g/L. A.7.2.6 sodium hydroxide solution. 100g/L. A.7.3 Instruments and Equipment A.7.3.1 Spectrophotometer. A.7.3.2 chromatography filter paper. No. 1 in speed, 150mm × 250mm. A.7.3.3 chromatography tank. φ240mm × 300mm. A.7.3.4 micro injector. 100μL. A.7.3.5 Nessler colorimetric tube. 50mL glass grinding mouth stopper. A.7.3.6 glass frit funnel. G3, pore size of 15μm ~ 40μm. A.7.3.7 50mm cuvette. A.7.3.8 10mm cuvette. A.7.4 Analysis step A.7.4.1 paper chromatographic conditions A.7.4.1.1 developing solvent. n-butanol ethanol solution of aqueous ammonia = 623. A.7.4.1.2 Temperature. 20 ℃ ~ 25 ℃. A.7.4.2 preparation of the sample solution Weigh 2g sample (accurate to 0.001g). Placed in a beaker, adding the right amount of water and 50mL of sodium hydroxide solution, heated to dissolve, Transferred to 100mL volumetric flask, dilute to volume, shake up the sample solution concentration of 2%. A.7.4.3 wash out the sample preparation liquid With micro-injector draw 100μL sample solution evenly note on the bottom edge of the filter paper from a baseline of 25mm, a straight line, Its width on the filter paper does not exceed 5mm, length 130mm, with a hair dryer. The filter paper containing preformulated good Expand Chromatography tank agent, expand, filter paper dipped under the bottom edge of the agent level l0mm, to be solvent front line rose to 150mm or until the dye deputy Material separation satisfaction. Remove the filter paper chromatography, with cold dry. Blank filter paper under the same conditions to expand the blank paper and the above steps should be expanded with the adjacent portion of the paper on the same piece of filter paper Bit clipping. Deputy dye paper chromatography is shown in Figure A.1. The respective deputy dye expanded and made on a blank paper to each subsidiary colors corresponding parts of the paper in the same size cut, And cut into thin strips about 5mm × 15mm, and were placed in 50mL of Nessler colorimetric tube, accurately added to the acetone solution 5mL, shake 3min ~ 5min, then the exact solution of sodium bicarbonate was added 20mL, shake well, and then were naturally filtered G3 sintered glass funnel, and the filtrate We should clarify that no suspension. Respectively each subsidiary colors and blank eluate. In the respective maximum absorption wavelength of the dye deputy, with 50mm Cuvette wash the dye out of each sub-liquid measuring their absorbance on a spectrophotometer. Absorbance values measured on a spectrophotometer, with a mixture of 5mL and 20mL acetone solution of sodium bicarbonate solution as a reference solution. A.7.4.4 preparation of standard solution Draw sample solution 6mL 2% moved into 100mL volumetric flask, dilute to the mark, shake, and the solution as the standard solution. A.7.4.5 Preparation of standard eluate With micro-injector draw 100μL standard solution, uniform injection site on the bottom edge of the filter paper from a baseline of 25mm with a hair dryer Dry. The filter paper containing previously prepared well eluent chromatography tank to expand, to be solvent front line up 40mm, remove with cold Wind and dry, cut out all the dye partially deployed, according to A.7.4.3 subjected to extraction methods to obtain standard eluate. Colorimetric with 10mm Dish at the maximum absorption wavelength measured absorbance values. Meanwhile blank filter paper under the same conditions to start operating in the same manner after washing the absorbance value measured liquid. Baseline Vice-dye (2) Main dye Deputy dye (1) 130mm 150mm 250mm 25m m Figure A.1 deputy dye paper chromatography schematic A.7.4.6 Calculation Results Deputy dye mass fraction 4w and its value is expressed in%, according to formula (A.4) Calculated. () () [] S bA bAbAw ss nn × - - - = ) 6/100) (( LL (A.4) Where. A1An - each sub-dye eluate 50mm optical path length measured absorbance values; b1bn - each sub-dye control blank eluate 50mm optical path length measured absorbance values; As - Standard eluate 10mm path length measured absorbance values; bs - standard control blank eluate 10mm path length measured absorbance values; 5 - converted to 10mm in number than the optical path length; 100/6 - Standard eluate converted into a 2% solution of the sample number ratio; S - mass fraction% of the sample. The results represent a decimal. The absolute difference between the parallel determination results is not more than 0.2% (mass fraction), the arithmetic mean value as a measurement result. A.8 Determination of sodium iodide A.8.1 Method summary By potentiometric titration with standard silver nitrate content of the solution titrated sample titration of sodium iodide. A.8.2 Reagents and materials Standard silver nitrate titration solution. c (AgNO3) = 0.001mol/L. A.8.3 Instruments and Equipment A.8.3.1 digital millivolt meter. A.8.3.2 iodide ion selective electrode. A.8.3.3 reference electrode. A.8.3.4 magnetic stirrer. A.8.4 Preparation of sample solution Weigh about 2.0g sample (accurate to 0.0001 g), placed in a beaker, was added 100mL of water were accurately taken, magnetic stirrer After stirring, filled with dry filter paper in a glass funnel crucible sand filtration, the filtrate as the sample solution. A.8.5 Analysis step The iodide ion selective electrode and a reference electrode is inserted into the test solution, and then adjust the millivolt millivolt meter readings in full stirring, Standard silver nitrate titration solution titration. At the start of the titration titration every 0.5mL, gradually added, and then observe the potential of dropping each variable Of, and record the reading of the potential, when close to the end, dropping down to each 0.1mL, the measured potential and the corresponding millivolt readings The maximum jump point titration volume plotted standard silver nitrate titration solution, titration curve is the end, come to the corresponding standard silver nitrate Volume of titrant. A.8.6 Calculation Results 5w mass fraction of sodium iodide and its value is expressed in%, according to formula (A.5) Calculated. 0) 1000/( 5 × = m MVc w (A.5) Where. c - accurate standard silver nitrate titration solution concentration value in units of moles per liter (mol/L); V - accurate value of the sample consumed titration standard silver nitrate titration solution volume in milliliters (mL); M - molar mass values of sodium iodide, in units of grams per mole (g/mol) [M (NaI) = 149.89]; Mass values m6-- sample in grams (g). The results represent a decimal A.9 Determination of Arsenic A.9.1 Method summary Erythrosine aluminum lake after wet digestion to prepare a sample solution, determination of arsenic by atomic absorption spectrometry. A.9.2 Reagents and materials A.9.2.1 nitrate. A.9.2.2 sulfuric acid solution. 11. A.9.2.3 nitric acid - perchloric acid mixed solution. 31. A.9.2.4 arsenic (As) standard solution. According to GB/T 602 formulation and calibration, and then diluted formulated to contain the instrument according to the requirements of use Three corresponding concentration of arsenic standard solution. A.9.2.5 sodium hydroxide solution. 1g/L. A.9.2.6 sodium borohydride. 8g/L (solvent 1g/L sodium hydroxide solution). A.9.2.7 hydrochloric acid solution. 110. A.9.2.8 potassium iodide solution.200g/L. A.9.3 Instruments and Equipment A.9.3.1 atomic absorption spectrometer. A.9.3.2 Instrument Reference conditions. arsenic hollow cathode lamp analytical line wavelength. 193.7nm; slit. 0.5nm ~ 1.0nm; lamp current. 6mA ~ 10mA. A.9.3.3 carrier gas flow rate. Argon gas 250mL/min. A.9.3.4 atomizer temperature. 900 ℃. A.9.4 Analysis step A.9.4.1 sample digestion Weigh about 1g sample (accurate to 0.001g), placed in 250mL triangular or round-bottomed flask, add 10mL ~ 15mL of nitric acid and 2mL Sulfuric acid solution, after shaking out of the nitrogen dioxide gas is heated over low heat, solution turned brown, stop heating, add 5mL nitric acid to cool - Perchloric acid mixture, strong heat until the solution became clear or slightly yellow, is still not as transparent, add 5mL nitric acid supplemented After cooling - perchloric acid mixed Solution, heating was continued until the solution is clear and colorless or slightly yellow and white smoke (to avoid charring dry out phenomenon occurs), heating was stopped and allowed to cool after 5mL water heated to boiling, remove residual nitric acid - perchloric acid (if necessary, can be combined with water to boil once), continue heating until white smoke occurred, Paul Holding 10min, transferred to 100mL volumetric flask After cooling (if the solution appears cloudy, precipitation or mechanical impurities to be filtered), diluted with hydrochloric acid solution Volume. At the same time, prepare a blank solution in the same manner. A.9.4.2 Determination Measure 25mL sample solution after digestion to 50mL volumetric flask, add 5mL potassium iodide solution with hydrochloric acid solution was diluted to volume, shake, Stand for 15min. At the same time in the same manner to prepare a blank solution blank test solution. Turn on the instrument, and the instrument is fully preheated arsenic hollow cathode lamp, a stable baseline, with a solution of sodium borohydride as hydride reduction occurs Agent to order standard blank, standard solution, sample solution and the blank test sample solution, according to computer instructions injections, respectively. End of test After the computer automatically generate the curve and deduct the arsenic concentration of the sample solution after sample blank, enter sample information (name, sample weight, Dilution volume, etc.), automatically converted from arsenic content in the sample. The absolute difference between the parallel determination results is not more than 0.1mg/kg, the arithmetic mean as a measurement result. A.10 Determination of Lead A.10.1 Method summary Erythrosine aluminum lake after wet digestion to prepare a sample solution, Determination of lead by atomic absorption spectrometry. A.10.2 reagents and materials A.10.2.1 Lead (Pb) standard solution. According to GB/T 602 formulation and calibration, and then according to the requirements of the instrument used in diluted formulated to contain Three corresponding concentration of lead standard solution. A.10.2.2 sodium hydroxide solution. 1g/L. A.10.2.3 sodium borohydride. 8g/L (solvent 1g/L sodium hydroxide solution). A.10.2.4 hydrochloric acid solution. 110. A.10.3 instruments and equipment A.10.3.1 atomic absorption spectrometer. A.10.3.2 instrument reference conditions. GB 5009.12-2010 third flame atomic absorption spectrometry. A.10.4 Analysis steps A.9.4.1 can be directly used in the sample solution and blank solution. Press GB 5009.12-2010 third flame atomic absorption spectrometry operations. The absolute difference between the parallel determination results is not more than 1.0mg/kg, the arithmetic mean as a measurement result. Determination of zinc A.11 A.11.1 Method summary Erythrosine aluminum lake after wet digestion to prepare the sample solution by atomic absorption spectrometry determination of zinc content. A.11.2 reagents and materials A.11.2.1 zinc (Zn) standard solution. According to GB/T 602 formulation and calibration, and then according to the requirements of the instrument used in diluted formulated to contain Three corresponding concentration of zinc standard solution. A.11.2.2 sodium hydroxide solution. 1g/L. A.11.2.3 sodium borohydride. 8g/L (solvent 1g/L sodium hydroxide solution). A.11.2.4 hydrochloric acid solution. 110. A.11.3 instruments and equipment A.11.3.1 atomic absorption spectrometer. A.11.3.2 instrument reference conditions. GB/T 5009.14-2003 first Atomic Absorption Spectrometry. A.11.4 Analysis steps A.9.4.1 can be directly used in the sample solution and blank solution. According to GB/T 5009.14-2003 first atomic absorption spectrometry determination. The absolute difference between the parallel determination results is not more than 5.0mg/kg, the arithmetic mean as a measurement result. Determination of barium A.12 A.12.1 Method summary Erythrosine aluminum lake after the dry digestion process to prepare a sample solution, a solution of barium compared to the standard, the turbidity limit for barium sulfate test. A.12.2 reagents and materials A.12.2.1 sulfuric acid. A.12.2.2 anhydrous sodium carbonate. A.12.2.3 hydrochloric acid solution. 13. A.12.2.4 sulfuric acid solution. 119. A.12.2.5 barium standard solution. barium chloride (BaCl2 · 2H2O) 177.9mg, dissolved in water and dilute to 1000mL. 0 per milliliter. 1 mg of barium (0.1mg/mL). A.12.3 preparation of the sample solution Weigh about 1g sample (accurate to 0.001g), placed in a platinum crucible or a ceramic crucible, add a small amount of sulfuric acid wetting, slowly heating, Try to make almost all of carbonization at low temperatures. After cooling, plus 1mL of sulfuric acid, heated slowly to hardly occurs until the sulfuric acid vapor, Placed in a furnace at 800 ℃ burning 3h. After cooling, add 5g anhydrous sodium carbonate were thoroughly mixed with official placed in a furnace at 860 ℃ burning 15min, after cooling, add water, 20mL, heated in water bath, dissolve the melt. After cooling and filtration, the filter was washed with water The residue was washed paper to sulfate was not reacted. The paper is then moved together with the filter paper and residue beaker, add 30mL salt Acid solution, shake it well boiled. After cooling and filtration, the residue was washed with 10mL of water on the filter paper. The washings were combined with the filtrate, in Evaporated to dryness on a water bath. The residue was dissolved 5mL water added, if necessary, by filtration, the solution added 0.25mL of hydrochloric acid, after mixing, add water To 25mL with a sample solution. A.12.4 turbidity standard solution preparation Take 5mL barium standard solution, add a solution of hydrochloric acid 0.25mL. Add water to 25mL, as a standard turbidity solution. A.12.5 Analysis steps In each sample solution and the standard solution of sulfuric acid plus 1mL mixed turbidity solution, placed 10min, the sample solution must not exceed the degree of opacity By standard turbidity solution is qualified.

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