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GB 15193.19-2015 English PDF

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GB 15193.19-2015: National food safety standard -- Methods for disposing mutagens, teratogens and carcinogens
Status: Valid

GB 15193.19: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB 15193.19-2015English209 Add to Cart 3 days [Need to translate] National food safety standard -- Methods for disposing mutagens, teratogens and carcinogens Valid GB 15193.19-2015
GB 15193.19-2003English199 Add to Cart 2 days [Need to translate] Methods for disposing mutagens, teratogens and carcinogens Obsolete GB 15193.19-2003
GB 15193.19-1994English199 Add to Cart 2 days [Need to translate] Methods for disposing mutagen, teratogens and carcinogens Obsolete GB 15193.19-1994

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Basic data

Standard ID GB 15193.19-2015 (GB15193.19-2015)
Description (Translated English) National food safety standard -- Methods for disposing mutagens, teratogens and carcinogens
Sector / Industry National Standard
Classification of Chinese Standard C53
Classification of International Standard 7.1
Word Count Estimation 10,145
Date of Issue 2015-08-07
Date of Implementation 2015-10-07
Older Standard (superseded by this standard) GB 15193.19-2003
Regulation (derived from) National Food Safety Standard Announcement 2015 No.6
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China
Summary This standard specifies the laboratory mutagens, teratogenic and carcinogenic treatment methods. This standard is applicable to the treatment of mutagens, teratogens and carcinogens used in food safety toxicology evaluation methods.

GB 15193.19-2015: National food safety standard -- Methods for disposing mutagens, teratogens and carcinogens


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(National food safety standards caused by treatment mutagens, teratogens and carcinogens) National Standards of People's Republic of China National Food Safety Standard Mutagens, teratogens and carcinogens Approach Issued on.2015-08-07 2015-10-07 implementation People's Republic of China National Health and Family Planning Commission released

Foreword

This standard replaces GB 15193.19-2003 "mutagens, teratogens and carcinogens approach." This standard compared with GB 15193.19-2003, the main changes are as follows. --- Standard name was changed to "national food safety standards mutagens, teratogens and carcinogens approach"; --- Revised range. --- Increased test purposes, the "general principles" to "principle", delete the original content "to make such substances destroyed chemical reaction Treatment, such as a compound susceptible to oxidation (such as hydrazine, or aromatic amines containing carbon-carbon double bonds separated = compound), can be saturated Potassium permanganate in acetone (1000mL dissolved in acetone 15g potassium permanganate) solution. Alkylate in principle with a suitable Affinity agent, such as water, hydroxide ions, ammonia, and other sulfite and thiosulfate reacts been destroyed, "was changed to" a number of chemical reactions Should destroy such substances caused mutagenic, teratogenic and carcinogenic effects of functional groups, so as to achieve food safety toxicology evaluation The method used mutagens, teratogens and carcinogens harmless treatment purposes. " --- Revised approach benzidine, β- naphthylamine. --- Increases the processing method of sodium azide. --- Modify the processing method of aflatoxin B1. --- Modify the benzo [a] anthracene name and processing method, the name of 3-methyl gall anthracene, processing method and abbreviation. --- Modified 2-acetylamino-fluorene, 2,7-diamino fluorene, benzo [a] pyrene approach. --- Increased 7,12-dimethyl-benz [a] anthracene, 2-amino-fluorene, 2-nitro-fluorene, anthracene-9,10-dimethyl, 2,4,7-trinitro-fluorenone processing method. --- Modify the N- nitroso-methyl urea processing method and abbreviation, N- methyl -N- nitro-nitrosoguanidine -N- name and Treatment method. --- Increasing the processing method of Ethylnitrosourea. --- Modify the treatment of mitomycin C. --- Changing the name of N- nitroso dimethylamine, abbreviation and treatment methods. --- Modified ethyl methane sulfonate, methyl methanesulfonate name and processing method. --- Modify the processing method of ochratoxin A. --- Revised approach ICR-170, the name of triethylene melamine and treatment. --- Increased 4-nitro-quinoline -N- oxide, ICR-191, furanose amide, 9-amino acridine, treatment of PCBs. --- Revised approach cyclophosphamide. --- Increasing the Congo red approach. --- Increases the processing method dimethylaminobenzaldehyde diazonium sulfonate. --- Increasing the processing method of sodium pentachlorophenol. --- Increase the cumene hydroperoxide approach. --- Increased daunorubicin treatment. --- Removed ethyleneimine, Trenimone approach. --- Added Appendix A. National Food Safety Standard Mutagens, teratogens and carcinogens Approach

1 Scope

This standard specifies the laboratory mutagens, teratogens and carcinogens approach. This standard applies to food safety toxicology evaluation methods used mutagens, teratogens and carcinogens treatment.

2 test purposes and principles

For most types of mutagens, teratogens and carcinogens, could use some kind of chemical reaction damage caused by mutagenic substances Change, teratogenic and carcinogenic effects of functional groups, so as to achieve food safety toxicology evaluation methods used mutagens, teratogens and cause Carcinogen treatment and disposal purposes.

3 Processing Method

Note. This chapter relates mutagens, teratogens and carcinogens in the English name of the control, CAS and the initials refer to Appendix A. 3.1 benzidine, β- naphthylamine 3.1.1 Reagents 2mol/L sulfuric acid, 0.2mol/L potassium permanganate, sodium metabisulfite, 10mol/L of potassium hydroxide. 3.1.2 Processing Method 3.1.2.1 such as benzidine or β- naphthylamine concentrations higher than 0.9mg/mL, diluted with water to 0.9mg/mL or less. 3.1.2.2 Each 10mL benzidine or β- naphthylamine was added 5mL0.2mol L potassium permanganate solution/and 5mL2mol/L sulfuric acid solution Liquid, the reaction should be continued for at least 10h. 3.1.2.3 Each 10mL reaction system was added 0.8g of sodium metabisulfite decolorization of the reaction system, if not completely decolorized then add more. 3.1.2.4 Each reaction system mentioned above was added 10mL 8mL10mol/L of potassium hydroxide, the reaction system is strongly alkaline (pH > 12), the trans Should exothermic process. 3.1.2.5 plus diluted with 100mL of water, filtered manganese compound, and the filtrate after the chemical waste in accordance with waste disposal. Sodium azide 3.2 3.2.1 Reagents Sodium nitrite, 4mol/L of sulfuric acid, the volume ratio of mass concentration of 10% potassium iodide solution, 1mol/L hydrochloric acid, starch. 3.2.2 solution preparation Sodium nitrite solution. 7.5g of sodium nitrite in 38mL water. 3.2.3 Processing Method 3.2.3.1 Each 5g of sodium azide were dissolved in 100mL of water. 3.2.3.2 sodium nitrite solution with stirring to a solution of sodium azide (3.2.3.1) in. 3.2.3.3 slowly added 4mol/L sulfuric acid until acidic reaction system and the stirring was continued for 1h, such as more samples, the process proceeds with an ice bath Carried out this operation need to be carried out in a fume hood. 3.2.3.4 should be sodium nitrite, and then adding sulfuric acid, was added in reverse order will produce harmful substances. 3.2.3.5 Add a few drops of the reaction solution to a system of equal volume mass concentration of 10% by volume potassium iodide solution was added 1 drop of 1mol/L Hydrochloric acid and 1 drop of the starch solution. If the reaction solution dark blue shows the presence of excess nitrite that the reaction has been completed, in accordance with the waste chemical waste disposal Management. If you are not adding more excess sodium nitrite. 3.3 Aflatoxin B1 3.3.1 Reagents 5.25% sodium hypochlorite, acetone. 3.3.2 Processing Method 3.3.2.1 mg of aflatoxin per 2mL5.25% sodium hypochlorite solution was added, the reaction system was allowed to stand overnight, sodium hypochlorite solution at any time Room slow decomposition, should be detected in the active chlorine content of the solution at regular intervals. 3.3.2.2 After addition of 3 volumes of water and diluted with a total volume equivalent to 5% of acetone, the reaction of at least 30min. After the process is completed in accordance with waste Chemical waste. 3.4 PAHs (benzo [a] pyrene, 7,12-dimethyl-benz [a] anthracene, 3-methyl gall anthracene, 2-amino-fluorene, 2,7-diamino fluorene, 2-acetamido Fluorene, benz [a] anthracene, 2-nitrofluorene, 9,10-dimethyl anthracene, trinitro fluorenone) 3.4.1 Reagents Potassium permanganate, sulfuric acid, sodium metabisulfite, 10mol/L of potassium hydroxide and acetone. 3.4.2 preparation of sulfuric acid solution of potassium permanganate Added 1L3mol/L sulfuric acid in 47.4g of potassium permanganate, stirring 15min ~ 60min backup, using now. 3.4.3 Processing Method 3.4.3.1 Each 5mg PAHs added 2mL acetone and ensure that polycyclic aromatic hydrocarbons completely dissolved. 3.4.3.2 Each 5mg PAHs added 10mL sulfuric acid solution of potassium permanganate, the reaction system should be stirred for 1h. The need to maintain throughout the reaction The reaction system was purple, purple if not more potassium permanganate solution of sulfuric acid should be added until the reaction system was kept above purple 1h. 3.4.3.3 Each 10mL reaction system was added 0.8g of sodium metabisulfite decolorization of the reaction system, if not completely decolorized then add more. 3.4.3.4 Each reaction system was added 10mL 8mL10mol/L of potassium hydroxide, the reaction system is strongly alkaline (pH > 12), through the reaction Exothermic process. 3.4.3.5 plus diluted with 100mL of water, filtered manganese compound, and the filtrate after the chemical waste in accordance with waste disposal. 3.4.3.6 such as polycyclic aromatic hydrocarbons less than 5mg, should also be addressed in accordance with the method 3.4.3.1 ~ 3.4.3.5. 3.5 N- methyl -N- -N- nitro-nitrosoguanidine, N- nitroso-methyl urea, Ethylnitrosourea 3.5.1 Reagents Methanol, 6mol/L hydrochloric acid, sulfamic acid. 3.5.2 Processing Method 3.5.2.1 Each 30g methyl - nitro - nitrosoguanidine, N- nitroso-methyl urea or Ethylnitrosourea added 1L of methanol. 3.5.2.2 added slowly with stirring and 1L6mol/L hydrochloric acid. 3.5.2.3 sulfamic acid 70g was added, the reaction system was stirred for at least 24h. Waste water treatment in accordance with the chemical waste after processing is completed. 3.6 mitomycin C 3.6.1 Reagents 5.25% sodium hypochlorite, 1% sodium hydrogen sulfite. 3.6.2 Processing Method 3.6.2.1 As the concentration is too high, it should be diluted mitomycin C to not more than 0.5mg/mL. 3.6.2.2 Each 10mL mitomycin C was added 15mL5.25% sodium hypochlorite solution, the reaction process is very fast. 3.6.2.3 After the completion of the reaction was added excess 1% sodium bisulfite to remove the excess sodium hypochlorite. After the process is completed in accordance with the waste chemical waste deal with. 3.7 N- NDMA 3.7.1 Reagents Methanol, 1mol/L of potassium hydroxide, an aluminum-nickel alloy. 3.7.2 Processing Method 3.7.2.1 The water (or methanol) N- nitroso dimethylamine was dissolved to a concentration of not more than 10mg/mL. 1mol/L potassium hydroxide solution was added an equal volume of 3.7.2.2 and using a magnetic stirrer. 3.7.2.3 Each 100mL reaction solution was slowly added 5g of aluminum-nickel alloy, should prevent excessive foaming, this exothermic reaction should be less than the total volume of container Reactor volume third. 3.7.2.4 reaction system was closed and stirring was continued for 24h. Discard 3.7.2.5 spent nickel can be put in dry 24h metal bracket (away from combustible materials) later. After the process is completed in accordance with the waste chemical waste deal with. 3.8 methyl methanesulfonate, ethyl methanesulfonate 3.8.1 Reagents 1mol/L NaOH. 3.8.2 Processing Method Each compound was added 1mL 50mL1mol/L sodium hydroxide solution, the reaction system should methyl methanesulfonate was stirred 6h, ethyl methanesulfonate 48h. Waste water treatment in accordance with the chemical waste after processing is completed. 3.9 Ochratoxin A 3.9.1 Reagents Sodium hypochlorite, ethanol. 3.9.2 solution preparation Sodium hypochlorite solution. 100mL5.25% sodium hypochlorite was added 200mL of water into the preparation of the appropriate concentration of sodium hypochlorite solution prepared Use, using now. 3.9.3 Processing Method 3.9.3.1 Each 1mg ochratoxin A was dissolved in 1mL ethanol and 1mL each dissolved in ethanol ochratoxin A was added 50mL times Chlorite solution. 3.9.3.2 using sonication to make it fully dissolved, the system should reflect at least 30min. After the process is completed in accordance with the waste chemical waste deal with. 3.10 heterocyclic aromatics (4-nitroquinoline-oxide -N-, ICR-170, ICR-191, triethylene melamine, furan sugar amide, 9-amino acridine, and more Polychlorinated biphenyls) 3.10.1 Reagent Acetonitrile, sulfuric acid, potassium permanganate, sodium metabisulfite, 10mol/L of sodium hydroxide. 3.10.2 sulfuric acid solution of potassium permanganate preparation Added 1L3mol/L sulfuric acid in 47.4g of potassium permanganate, stirring 15min ~ 60min backup, using now. 3.10.3 Processing Method 3.10.3.1 heterocyclic aromatic per 5mg added 3mL of acetonitrile and make sure it is completely dissolved. 3.10.3.2 Each 5mg heterocyclic aromatic sulfuric acid was added potassium permanganate solution 10mL, and the reaction was stirred for 1h; during the reaction of the system should be maintained Purple, such as color fade should continue adding potassium permanganate solution of sulfuric acid, the reaction system was kept until the purple 1h more. 3.10.3.3 Each 10mL reaction system was added 0.8g of sodium metabisulfite decolorization of the reaction system, if not completely decolorized then add more. Each reaction system was added 10mL 3.10.3.4 8mL10mol/L of potassium hydroxide, the reaction system is strongly alkaline (pH > 12), through the reaction Exothermic process. 3.10.3.5 add 100mL diluted with water, filtered manganese compound, and the filtrate after the chemical waste in accordance with waste disposal. 3.10.3.6 heterocyclic aromatics such as less than 5mg, should also be addressed in accordance with 3.10.3.1 ~ 3.10.3.5 methods. 3.11 cyclophosphamide 3.11.1 Reagent 1mol/L hydrochloric acid, sodium thiosulfate, sodium hydroxide. 3.11.2 Processing Method 3.11.2.1 added per 250mg cyclophosphamide 10mL1mol/L hydrochloric acid, after cooling to room temperature was heated at reflux for 1h. 3.11.2.2 Add 0.2g/mL sodium hydroxide solution to pH 6, was cooled to room temperature. 3.11.2.3 per 250mg cyclophosphamide 1.5g sodium thiosulfate and 0.2g/mL of sodium hydroxide solution is strongly alkaline, the system should Reaction of at least 1h, diluted with water after completion of the reaction discarded. 3.12 Congo red 3.12.1 Reagent AmberliteXAD-16 resin. 3.12.2 Processing Method The 1gAmberliteXAD-16 resin was added 20mL of Congo Red concentration 100μg/mL solution and stirred for at least 2h. Waste and chemical waste in accordance with the resin treatment after treatment is completed. 3.13 dimethylaminobenzaldehyde diazonium sulfonate Heated to above 200 ℃ can be decomposed properly handle the waste gas. 3.14 sodium pentachlorophenol At 600 ℃ ~ 900 ℃ burning, and properly handle the waste gas. 3.15 cumene hydroperoxide Rotary kiln incineration, the temperature range of 820 ℃ ~ 1600 ℃, burning a few seconds to properly handle the waste gas. 3.16 daunorubicin Rotary kiln incineration, the temperature range of 820 ℃ ~ 1600 ℃, liquid incineration seconds, burning solid hours required to properly handle the waste gas.

Appendix A

Mutagens, teratogens and carcinogens in control English name, CAS and abbreviation Mutagens, teratogens and carcinogens in control English name, CAS and abbreviation in Table A.1. Table A.1 mutagens, teratogens and carcinogens in control English name, CAS and abbreviation Chinese name English name CAS abbreviation 2-acetamido-fluorene 2-acetamidofluorene 53-96-3 2-AAF Aflatoxin B1 aflatoxinB1 1162-65-8 AFB1 Benzo [a] anthracene, benz [a] anthracene 56-55-3 BA Benzidine benzidine 92-87-5 Bz Benzo [a] pyrene, benzo [a] pyrene 50-32-8 BP Cyclophosphamide cyclophosphamide 50-18-0 CP Amino-2,7-fluorene 2,7-diaminofluorene 525-64-4 2,7-AF 7,12-dimethyl-benz [a] anthracene-7,12-dimethylbenz [a] anthracene 57-97-6 DMBA Ethyl methane sulfonate ethylmethanesulphonate 62-50-0 EMS 3-methyl-MCA 3-methylcholanthrene 56-49-5 3-MC Methyl methanesulfonate methylmethanesulfonate 66-27-3 MMS Mitomycin C mitomycinC 50-07-7 MMC β- naphthylamine 2-naphthylamine 91-59-8 2-NAP Ethylnitrosourea N-nitroso-N-ethylurea 759-73-9 ENU N- methyl -N- -N- nitro-nitrosoguanidine N-methyl-N'-nitro-N-nitrosoguanidine 70-25-7 MNNG N- nitroso-methyl urea N-nitroso-N-methylurea 684-93-5 MNU 2-amino-fluorene 2-aminofluorene 153-78-6 - -N- 4- nitroquinoline-oxide 4-nitroquinoline-N-oxide 56-57-5 - N- NDMA N-nitrosodimethylamine 62-75-9 NDMA Ochratoxin A ochratoxinA 303-47-9 OA Sodium azide sodiumazide 26628-22-8 - ICR-170 ICR-170 146-59-8 - ICR-191 ICR-191 17070-45-0 - Triethylene melamine triethylenemelamine 51-18-3 - Furanose amide furylfuramide 3688-53-7 AF-2 9-amino acridine 9-aminoacridine 90-45-9 - 2-nitro-fluoren-2-nitrofluorene 607-57-8 - Anthracene-9,10-dimethyl-9,10-dimethylanthracene 781-43-1 - Trinitro fluorenone, 2,4,7-trinitro-9H-fluoren-9-one 129-79-3 - Table A.1 (CONTINUED) Chinese name English name CAS abbreviation Congo Red congored 573-58-0 - Dimethylaminobenzaldehyde diazonium sulfonate p ara-dimethylaminobenzenediazo sodiumsulfonate 140-56-7-- Sodium pentachlorophenol sodiumpentachlorophenolate 131-52-2 - Cumene hydroperoxide cumenehydroperoxide 80-15-9 - Daunorubicin daunorubicin 20830-81-3 - PCBs polychorinatedbiphenyls 1336-36-3 -

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