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QB/T 2738-2023: Evaluating methods for antibacterial and bacteriostatic efficacy of daily chemical products Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid QB/T 2738: Historical versions
Similar standardsQB/T 2738-2023: Evaluating methods for antibacterial and bacteriostatic efficacy of daily chemical products---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/QBT2738-2023 QB LIGHT INDUSTRY STANDARD OF THE PEOPLE��S REPUBLIC OF CHINA ICS 71.100.40 CCS Y 40 Replacing QB/T 2738-2012 Evaluating methods for antibacterial and bacteriostatic efficacy of daily chemical products Issued on. APRIL 21, 2023 Implemented on. NOVEMBER 01, 2023 Issued by. Ministry of Industry and Information Technology of PRC Table of ContentsForeword... 3 1 Scope... 5 2 Normative references... 5 3 Terms and definitions... 5 4 Basic requirements for laboratories and aseptic operations for testing the effects of antibacterial and bacteriostatic daily chemical products... 7 5 Sample collection... 8 6 Principles for evaluating the antibacterial and bacteriostatic efficacy of daily chemical products... 8 7 Test methods for antibacterial and bacteriostatic efficacy of daily chemical products ... 9 8 Stability test method for antibacterial and bacteriostatic efficacy of daily chemical products... 38 Appendix A (Informative) Statistical test examples... 39 References... 421 ScopeThis document describes the detection method of the antibacterial and bacteriostatic efficacy of daily chemical products with special hygiene functions; stipulates the evaluation criteria. This document is applicable to the testing and evaluation of the antibacterial and bacteriostatic properties of common detergents. The testing and evaluation of the antibacterial and bacteriostatic properties of other daily chemical products are selected according to the purpose.2 Normative referencesThe contents of the following documents constitute the essential terms of this document through normative references in the text. Among them, for dated references, only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable to this document. GB 4789.2 National food safety standard - Microbiological examination of food. Aerobic plate count QB/T 2153 Industrial oleic acid QB/T 2850 General technical requirements for antibacterial and bacteriostatic detergents3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Antibacterial The process of killing bacteria or hindering bacterial growth and activity by chemical or physical methods. 3.2 Bacteriostasis The process of inhibiting or hindering bacterial growth and activity by chemical or physical methods. 3.3 Carrier The support for test microorganisms. 3.4 Colony forming unit (CFU) In the counting of live bacteria, the colony expressing the number of live bacteria formed by the growth and reproduction of a single bacterium or multiple bacteria in a cluster on a solid culture medium. 3.5 Neutralizer In the microbial killing test, it is a reagent used to neutralize the suspension of the test microorganism and the bactericide and the residual bactericide on the surface of the microorganism, so that it loses its inhibitory and killing effects on the microorganism. 3.6 Product of neutralization The product after the neutralizer (3.5) reacts with the bactericide.4 Basic requirements for laboratories and aseptic operations for testing the effects of antibacterial and bacteriostatic dailychemical products 4.1 Microbiological laboratories shall adopt a closed layout; the building shall be easy to clean and disinfect. 4.2 Before the start of the test, the table and the indoor floor shall be cleaned by wet methods; then the air in the laboratory shall be disinfected by ultraviolet rays or other methods. 4.3 Experimenters shall wear work clothes, masks, hats; when conducting sterility tests, they shall enter the laboratory after air showering. Then, wear sterile isolation clothes, hats, masks correctly. 4.4 The sterile pipette shall be replaced every time a different sample is drawn; the inoculation loop (needle) shall be burned and sterilized on the flame before it can be used again. 4.6 Reagents that require sterility, such as distilled water, phosphate buffer, culture medium, bovine serum albumin, standard hard water, neutralizer, etc., shall be sterilized or filtered. 4.7 Before using sterile equipment and reagents, check whether the container or packaging is intact. If damaged, it shall not be used. 4.8 Sterile equipment and reagents in use shall not be exposed to the air for a long time. 4.9 When pipetting or inoculating, the mouth of the test tube and the agar plate shall be close to the flame to prevent contamination. 4.10 All used contaminated equipment shall be immediately placed in a container containing disinfectant, to prevent contamination of the surrounding environment and clean items.5 Sample collectionIn order to make the samples representative, at least 12 minimum sales packaging samples shall be randomly selected from the three transport packages of the same batch number, of which 4 samples shall be retained, 4 samples shall be tested for antibacterial or bactericidal performance, 4 samples shall be tested for stability.6 Principles for evaluating the antibacterial and bacteriostatic efficacy of daily chemical products6.1 Bactericidal test determination method The bactericidal test determination method in the document is used to evaluate the antibacterial effect of the product. 6.2 Bacteriostatic test determination method The bacteriostatic test determination method in the document is used to evaluate the bacteriostatic effect of the product. 6.3 Indicator microorganisms for inspection The test bacteria in Table 1 shall be selected and tested according to the product implementation standard or the scope of application of the instructions. Other strains can also be added as test strains according to the product usage requirements. 6.4 Action concentration It is carried out according to the standard requirements clearly stated on the product. If it is not clearly stated, QB/T 2850 shall be implemented. 6.5 Action time It is carried out according to the standard requirements clearly stated on the product. If it is not clearly stated, QB/T 2850 shall be implemented. 6.6 Colony count Count according to the provisions of GB 4789.2. 6.7 Result report The test result report is a written expression of the test conditions and results. The result report shall at least include.7 Test methods for antibacterial and bacteriostatic efficacyof daily chemical products 7.1 Classification of test methods for antibacterial and bacteriostatic efficacy of daily chemical products See Table 2 for the classification of test methods for antibacterial and bacteriostatic efficacy of daily chemical products. 7.2 Bactericidal effect test method for daily chemical products (suspension quantitative method) 7.2.1 Equipment The main equipment is. 7.2.2 Reagents 7.2.3 Neutralizer identification test 7.2.3.1 General In order to accurately evaluate the killing effect of the sample on microorganisms, it is required to select an appropriate neutralizer in the sterilization test. The selected neutralizer can not only stop the inhibitory and killing effect of the antibacterial wash on microorganisms in time, but also the reaction product of the neutralizer itself and the sample (i.e., the neutralization product) has no inhibitory or killing effect on microorganisms, has no adverse effect on the culture medium. 7.2.5 Calculation formula The sterilization rate is calculated according to formula (2); the result is expressed as a percentage (%), with two decimal places. 7.3 Bacteriostatic effect test method for daily chemical products (suspension quantitative method) 7.3.1 Equipment Same as 7.2.1. 7.3.2 Reagents Same as 7.2.2. 7.3.3 Bacteriostatic test operation steps 7.3.3.1 Dilute the test bacteria suspension (7.2.2.5) with PBS solution appropriately; the required concentration is as follows. take 0.1 mL and drop it into 5.0 mL control sample solution (PBS); the number of recovered bacteria is 1��104 CFU/mL ~ 9��104 CFU/mL. 7.3.4 Calculation formula The bacteriostatic rate is calculated according to formula (9); the result is expressed as a percentage (%), with 2 decimal places retained. 7.3.5 Error evaluation Same as 7.2.6. 7.3.6 Evaluation of product bacteriostatic effect Fill the test results into Table 7 to evaluate the bacteriostatic effect of the product. When the bacteriostatic rate is not less than 90%, it indicates that the product has a strong bacteriostatic effect under this condition; when the bacteriostatic rate is not less than 50% and less than 90%, it indicates that the product has an bacteriostatic effect under this condition. 7.4 Test method for antibacterial or bacteriostatic effect of fabric detergents (simulation method) 7.4.5 Operation steps of bactericidal or bacteriostatic test 7.4.5.1 Place 2 pieces of bacterial carrier (7.4.4.4) between the 6th and 7th layers of cloth strips of the rotating bracket; place the third piece between the 7th and 8th layers of cloth strips. 7.4.5.2 Aseptically place the rotating unit (bracket, cloth strip, contaminated carrier) into the glass jar containing the test sample and cover it. Fix the glass jar on the shaker; roll and rotate to wash until the set time (i.e., the action time of antibacterial and bacteriostatic tests); remove the glass jar. 7.5 Bacteriostatic effect test method for daily chemical products (bacteriostatic ring method) 7.5.1 Principle The bacteriostatic agent is continuously dissolved and diffused through agar, to form different concentration gradients to show its bacteriostatic effect. The size of the bacteriostatic ring is used to determine whether it has bacteriostatic ability. 7.5.2 Equipment 7.5.3 Reagents 7.5.4 Bacteriostatic test operation steps 7.5.4.1 Preparation of bacteriostatic tablets For liquid bacteriostatic test samples, take sterile dry filter paper pieces; add 20 ��L of bacteriostatic test sample solution of actual use concentration to each piece; then place the filter paper piece flat in a clean sterile plate; open the cover and place it in a constant temperature oven (36 �� 1) ��C to dry, or place it at room temperature to dry naturally to prepare for use. For solid bacteriostatic test samples, directly make a round piece (block) with a diameter of 5 mm and a thickness of no more than 4 mm, to prepare for use. 7.6 Test method for bacteriostatic effect of human skin cleaning products (retention method) 7.6.1 Principle This test simulates skin conditions suitable for bacterial growth, reproduction and possible infection; uses a randomized, double-blind, paired comparison method to detect the retention antibacterial effect of human skin cleaning products on human skin, over a certain period of time. 7.6.2 Equipment 7.6.3 Reagents 7.6.3.1 Culture medium (commercial qualified commercial culture medium). Tryptone soy broth medium (TSB), Tryptone soy agar medium (TSA). 7.6.3.2 0.03 mol/L phosphate buffer (same as 7.2.2.3). 7.6.3.3 70% ~ 75% alcohol. 7.6.3.4 Sheep blood. 7.6.3.5 Fatty alcohol polyoxyethylene ether. 7.6.3.6 External antibiotic ointment. 7.6.4 Samples The test products are divided into 25 groups according to the sequence number. Each group has 2 samples, one is the test sample prepared according to the specified action concentration, whilst the other is the control sample without bacteriostatic agent. 7.6.5 Bacteriostatic test operation steps 7.9 Bacteriostatic effect test method for bacteriostatic daily chemical products (carrier immersion quantitative method) 7.9.1 Equipment Same as 7.2.1. 7.9.2 Reagents Same as 7.2.2. 7.9.3 Preparation of bacterial carrier Same as 7.8.3. 7.9.4 Bacteriostatic test operation steps 7.9.4.1 Dilute the test sample with sterile standard hard water to the specified concentration (soap products are diluted with distilled water).8 Stability test method for antibacterial and bacteriostatic efficacy of daily chemical products8.1 Test conditions 8.1.1 Natural retention sample. Place the original packaged sample at room temperature (15 ��C ~ 30 ��C) and sample it for the time specified in the product instructions, for sterilization or antibacterial performance determination. 8.1.2 Accelerated test. Place the original packaged sample in a (54 �� 2) ��C constant temperature box for 14 days, or in a (37 �� 2) ��C constant temperature box for 90 days, or in a 40 ��C ~ 45 ��C constant temperature box for 180 days, or in a 40 ��C ~ 45 ��C constant temperature box for 270 days; conduct a bactericidal or bacteriostatic performance test. 8.2 Test method Use the corresponding method in Table 2. 8.3 Evaluation standard 8.3.1 After the product is naturally retained, its bactericidal rate or bacteriostatic rate reaches the specified index value; the bactericidal or bacteriostatic effect of the product is valid for the natural retention time. 8.3.2 After the product is placed in a (54 �� 2) ��C constant temperature box for 14 days, its bactericidal rate or bacteriostatic rate reaches the specified index value; the bactericidal or bacteriostatic effect of the product is valid for one year. ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. 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