QB/T 2738-2023 PDF English
US$470.00 · In stock · Download in 9 secondsQB/T 2738-2023: Evaluating methods for antibacterial and bacteriostatic efficacy of daily chemical products Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid QB/T 2738: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| QB/T 2738-2023 | English | 470 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
Evaluating methods for antibacterial and bacteriostatic efficacy of daily chemical products
| Valid |
| QB/T 2738-2012 | English | 245 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
Test methods for evaluating daily chemical products in antibacterial and bacteriostatic efficacy
| Valid |
| QB/T 2738-2005 | English | RFQ |
ASK
|
6 days
|
Test methods for evaluating daily chemical products in antibacterial and bacteriostatic efficacy
| Obsolete |
Excerpted PDFs (Download full copy in 9 seconds upon purchase)PDF Preview: QB/T 2738-2023
QB/T 2738-2023: Evaluating methods for antibacterial and bacteriostatic efficacy of daily chemical products
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/QBT2738-2023
QB
LIGHT INDUSTRY STANDARD OF THE
PEOPLE��S REPUBLIC OF CHINA
ICS 71.100.40
CCS Y 40
Replacing QB/T 2738-2012
Evaluating methods for antibacterial and bacteriostatic
efficacy of daily chemical products
Issued on. APRIL 21, 2023
Implemented on. NOVEMBER 01, 2023
Issued by. Ministry of Industry and Information Technology of PRC
Table of Contents
Foreword... 3
1 Scope... 5
2 Normative references... 5
3 Terms and definitions... 5
4 Basic requirements for laboratories and aseptic operations for testing the effects of
antibacterial and bacteriostatic daily chemical products... 7
5 Sample collection... 8
6 Principles for evaluating the antibacterial and bacteriostatic efficacy of daily chemical
products... 8
7 Test methods for antibacterial and bacteriostatic efficacy of daily chemical products
... 9
8 Stability test method for antibacterial and bacteriostatic efficacy of daily chemical
products... 38
Appendix A (Informative) Statistical test examples... 39
References... 42
1 Scope
This document describes the detection method of the antibacterial and bacteriostatic
efficacy of daily chemical products with special hygiene functions; stipulates the
evaluation criteria.
This document is applicable to the testing and evaluation of the antibacterial and
bacteriostatic properties of common detergents. The testing and evaluation of the
antibacterial and bacteriostatic properties of other daily chemical products are selected
according to the purpose.
2 Normative references
The contents of the following documents constitute the essential terms of this document
through normative references in the text. Among them, for dated references, only the
version corresponding to that date is applicable to this document; for undated references,
the latest version (including all amendments) is applicable to this document.
GB 4789.2 National food safety standard - Microbiological examination of food.
Aerobic plate count
QB/T 2153 Industrial oleic acid
QB/T 2850 General technical requirements for antibacterial and bacteriostatic
detergents
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Antibacterial
The process of killing bacteria or hindering bacterial growth and activity by
chemical or physical methods.
3.2
Bacteriostasis
The process of inhibiting or hindering bacterial growth and activity by chemical or
physical methods.
3.3
Carrier
The support for test microorganisms.
3.4
Colony forming unit (CFU)
In the counting of live bacteria, the colony expressing the number of live bacteria
formed by the growth and reproduction of a single bacterium or multiple bacteria in
a cluster on a solid culture medium.
3.5
Neutralizer
In the microbial killing test, it is a reagent used to neutralize the suspension of the
test microorganism and the bactericide and the residual bactericide on the surface of
the microorganism, so that it loses its inhibitory and killing effects on the
microorganism.
3.6
Product of neutralization
The product after the neutralizer (3.5) reacts with the bactericide.
4 Basic requirements for laboratories and aseptic operations for testing the effects of antibacterial and bacteriostatic daily
chemical products
4.1 Microbiological laboratories shall adopt a closed layout; the building shall be easy
to clean and disinfect.
4.2 Before the start of the test, the table and the indoor floor shall be cleaned by wet
methods; then the air in the laboratory shall be disinfected by ultraviolet rays or other
methods.
4.3 Experimenters shall wear work clothes, masks, hats; when conducting sterility tests,
they shall enter the laboratory after air showering. Then, wear sterile isolation clothes,
hats, masks correctly.
4.4 The sterile pipette shall be replaced every time a different sample is drawn; the
inoculation loop (needle) shall be burned and sterilized on the flame before it can be
used again.
4.6 Reagents that require sterility, such as distilled water, phosphate buffer, culture
medium, bovine serum albumin, standard hard water, neutralizer, etc., shall be sterilized
or filtered.
4.7 Before using sterile equipment and reagents, check whether the container or
packaging is intact. If damaged, it shall not be used.
4.8 Sterile equipment and reagents in use shall not be exposed to the air for a long time.
4.9 When pipetting or inoculating, the mouth of the test tube and the agar plate shall be
close to the flame to prevent contamination.
4.10 All used contaminated equipment shall be immediately placed in a container
containing disinfectant, to prevent contamination of the surrounding environment and
clean items.
5 Sample collection
In order to make the samples representative, at least 12 minimum sales packaging
samples shall be randomly selected from the three transport packages of the same batch
number, of which 4 samples shall be retained, 4 samples shall be tested for antibacterial
or bactericidal performance, 4 samples shall be tested for stability.
6 Principles for evaluating the antibacterial and bacteriostatic efficacy of daily chemical products
6.1 Bactericidal test determination method
The bactericidal test determination method in the document is used to evaluate the
antibacterial effect of the product.
6.2 Bacteriostatic test determination method
The bacteriostatic test determination method in the document is used to evaluate the
bacteriostatic effect of the product.
6.3 Indicator microorganisms for inspection
The test bacteria in Table 1 shall be selected and tested according to the product
implementation standard or the scope of application of the instructions. Other strains
can also be added as test strains according to the product usage requirements.
6.4 Action concentration
It is carried out according to the standard requirements clearly stated on the product. If
it is not clearly stated, QB/T 2850 shall be implemented.
6.5 Action time
It is carried out according to the standard requirements clearly stated on the product. If
it is not clearly stated, QB/T 2850 shall be implemented.
6.6 Colony count
Count according to the provisions of GB 4789.2.
6.7 Result report
The test result report is a written expression of the test conditions and results. The result
report shall at least include.
7 Test methods for antibacterial and bacteriostatic efficacy
of daily chemical products
7.1 Classification of test methods for antibacterial and bacteriostatic efficacy of
daily chemical products
See Table 2 for the classification of test methods for antibacterial and bacteriostatic
efficacy of daily chemical products.
7.2 Bactericidal effect test method for daily chemical products (suspension
quantitative method)
7.2.1 Equipment
The main equipment is.
7.2.2 Reagents
7.2.3 Neutralizer identification test
7.2.3.1 General
In order to accurately evaluate the killing effect of the sample on microorganisms, it is
required to select an appropriate neutralizer in the sterilization test. The selected
neutralizer can not only stop the inhibitory and killing effect of the antibacterial wash
on microorganisms in time, but also the reaction product of the neutralizer itself and the
sample (i.e., the neutralization product) has no inhibitory or killing effect on
microorganisms, has no adverse effect on the culture medium.
7.2.5 Calculation formula
The sterilization rate is calculated according to formula (2); the result is expressed as a
percentage (%), with two decimal places.
7.3 Bacteriostatic effect test method for daily chemical products (suspension
quantitative method)
7.3.1 Equipment
Same as 7.2.1.
7.3.2 Reagents
Same as 7.2.2.
7.3.3 Bacteriostatic test operation steps
7.3.3.1 Dilute the test bacteria suspension (7.2.2.5) with PBS solution appropriately;
the required concentration is as follows. take 0.1 mL and drop it into 5.0 mL control
sample solution (PBS); the number of recovered bacteria is 1��104 CFU/mL ~ 9��104
CFU/mL.
7.3.4 Calculation formula
The bacteriostatic rate is calculated according to formula (9); the result is expressed as
a percentage (%), with 2 decimal places retained.
7.3.5 Error evaluation
Same as 7.2.6.
7.3.6 Evaluation of product bacteriostatic effect
Fill the test results into Table 7 to evaluate the bacteriostatic effect of the product. When
the bacteriostatic rate is not less than 90%, it indicates that the product has a strong
bacteriostatic effect under this condition; when the bacteriostatic rate is not less than
50% and less than 90%, it indicates that the product has an bacteriostatic effect under
this condition.
7.4 Test method for antibacterial or bacteriostatic effect of fabric detergents
(simulation method)
7.4.5 Operation steps of bactericidal or bacteriostatic test
7.4.5.1 Place 2 pieces of bacterial carrier (7.4.4.4) between the 6th and 7th layers of
cloth strips of the rotating bracket; place the third piece between the 7th and 8th layers
of cloth strips.
7.4.5.2 Aseptically place the rotating unit (bracket, cloth strip, contaminated carrier)
into the glass jar containing the test sample and cover it. Fix the glass jar on the shaker;
roll and rotate to wash until the set time (i.e., the action time of antibacterial and
bacteriostatic tests); remove the glass jar.
7.5 Bacteriostatic effect test method for daily chemical products (bacteriostatic
ring method)
7.5.1 Principle
The bacteriostatic agent is continuously dissolved and diffused through agar, to form
different concentration gradients to show its bacteriostatic effect. The size of the
bacteriostatic ring is used to determine whether it has bacteriostatic ability.
7.5.2 Equipment
7.5.3 Reagents
7.5.4 Bacteriostatic test operation steps
7.5.4.1 Preparation of bacteriostatic tablets
For liquid bacteriostatic test samples, take sterile dry filter paper pieces; add 20 ��L of
bacteriostatic test sample solution of actual use concentration to each piece; then place
the filter paper piece flat in a clean sterile plate; open the cover and place it in a constant
temperature oven (36 �� 1) ��C to dry, or place it at room temperature to dry naturally to
prepare for use. For solid bacteriostatic test samples, directly make a round piece (block)
with a diameter of 5 mm and a thickness of no more than 4 mm, to prepare for use.
7.6 Test method for bacteriostatic effect of human skin cleaning products
(retention method)
7.6.1 Principle
This test simulates skin conditions suitable for bacterial growth, reproduction and
possible infection; uses a randomized, double-blind, paired comparison method to
detect the retention antibacterial effect of human skin cleaning products on human skin,
over a certain period of time.
7.6.2 Equipment
7.6.3 Reagents
7.6.3.1 Culture medium (commercial qualified commercial culture medium). Tryptone
soy broth medium (TSB), Tryptone soy agar medium (TSA).
7.6.3.2 0.03 mol/L phosphate buffer (same as 7.2.2.3).
7.6.3.3 70% ~ 75% alcohol.
7.6.3.4 Sheep blood.
7.6.3.5 Fatty alcohol polyoxyethylene ether.
7.6.3.6 External antibiotic ointment.
7.6.4 Samples
The test products are divided into 25 groups according to the sequence number. Each
group has 2 samples, one is the test sample prepared according to the specified action
concentration, whilst the other is the control sample without bacteriostatic agent.
7.6.5 Bacteriostatic test operation steps
7.9 Bacteriostatic effect test method for bacteriostatic daily chemical products
(carrier immersion quantitative method)
7.9.1 Equipment
Same as 7.2.1.
7.9.2 Reagents
Same as 7.2.2.
7.9.3 Preparation of bacterial carrier
Same as 7.8.3.
7.9.4 Bacteriostatic test operation steps
7.9.4.1 Dilute the test sample with sterile standard hard water to the specified
concentration (soap products are diluted with distilled water).
8 Stability test method for antibacterial and bacteriostatic efficacy of daily chemical products
8.1 Test conditions
8.1.1 Natural retention sample. Place the original packaged sample at room temperature
(15 ��C ~ 30 ��C) and sample it for the time specified in the product instructions, for
sterilization or antibacterial performance determination.
8.1.2 Accelerated test. Place the original packaged sample in a (54 �� 2) ��C constant
temperature box for 14 days, or in a (37 �� 2) ��C constant temperature box for 90 days,
or in a 40 ��C ~ 45 ��C constant temperature box for 180 days, or in a 40 ��C ~ 45 ��C
constant temperature box for 270 days; conduct a bactericidal or bacteriostatic
performance test.
8.2 Test method
Use the corresponding method in Table 2.
8.3 Evaluation standard
8.3.1 After the product is naturally retained, its bactericidal rate or bacteriostatic rate
reaches the specified index value; the bactericidal or bacteriostatic effect of the product
is valid for the natural retention time.
8.3.2 After the product is placed in a (54 �� 2) ��C constant temperature box for 14 days,
its bactericidal rate or bacteriostatic rate reaches the specified index value; the
bactericidal or bacteriostatic effect of the product is valid for one year.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Tips & Frequently Asked QuestionsQuestion 1: How long will the true-PDF of English version of QB/T 2738-2023 be delivered?Answer: The full copy PDF of English version of QB/T 2738-2023 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice. Question 2: Can I share the purchased PDF of QB/T 2738-2023_English with my colleagues?Answer: Yes. The purchased PDF of QB/T 2738-2023_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- QB/T 2738-2023 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds. Question 5: Should I purchase the latest version QB/T 2738-2023?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version QB/T 2738-2023 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. How to buy and download a true PDF of English version of QB/T 2738-2023?A step-by-step guide to download PDF of QB/T 2738-2023_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "QB/T 2738-2023".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9
|