Search result: YC/T 282-2009
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Tobacco leaf. Determination of dissociated amino acids. Amino analyzer method
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YC/T 282-2009
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| Standard ID | YC/T 282-2009 (YC/T282-2009) | | Description (Translated English) | Tobacco leaf. Determination of dissociated amino acids. Amino analyzer method | | Sector / Industry | Tobacco Industry Standard (Recommended) | | Classification of Chinese Standard | X87 | | Classification of International Standard | 65.160 | | Word Count Estimation | 12,134 | | Date of Issue | 2009-03-30 | | Date of Implementation | 2009-05-01 | | Quoted Standard | YC/T 31-1996 | | Drafting Organization | Hubei tobacco industry limited liability company | | Administrative Organization | National Standardization Technical Committee of Tobacco | | Regulation (derived from) | 2009 No. 5 (total 113) Chinese industry standard filing Announcements | | Summary | This standard specifies the amino acids in tobacco amino acid analyzer method. This standard applies to tobacco aspartic acid (Asp), threonine (Thr), serine (Ser), asparagine (Asn), glutamic acid (Glu), proline (Pro), glycine (Gly), alanine (Ala), valine (Val), cystine (Cys), isoleucine (Ile), leucine (Leu), tyrosine (Tyr), phenylalanine (Phe), ��-alanine (��-Ala), ��-amino isobutyric acid (��-Aia), ��-aminobutyric acid (��-Aba), lysine (Lys), histidine (His), tryptophan (Trp), arginine (Arg) and other 21 kinds of free amino acids measured. |
YC/T 282-2009
Tobacco leaf.Determination of dissociated amino acids.Amino analyzer method
ICS 65.160
X87
Record Number. 25965-2009
People's Republic of China tobacco industry standards
Determination of free amino acids in tobacco
Amino acid analyzer method
Posted 2009-03-30
2009-05-01 implementation
State Tobacco Monopoly Bureau issued
Foreword
This standard Annex A, Annex B, Annex C is informative appendix.
This standard was proposed by the State Tobacco Monopoly Bureau.
This standard by the National Standardization Technical Committee on Tobacco Cigarette Technical Committee (SAC/TC144/SC1) centralized.
This standard was drafted. Hubei tobacco industry limited liability company, China National Tobacco Corporation, Zhengzhou Tobacco Research Institute.
The main drafters of this standard. Juan, Dan, Liu Jianfeng, Machou wing Songxu Yan, Guo Guoning, Liu Jian.
Determination of free amino acids in tobacco
Amino acid analyzer method
1 Scope
This standard specifies the amino acid analyzer method of free amino acids in tobacco leaves.
This standard applies to tobacco aspartate (Asp), threonine (Thr), serine (Ser), asparagine (Asn), glutamate (Glu),
Proline (Pro), glycine (Gly), alanine (Ala), valine (Val), cystine (Cys), isoleucine (Ile), leucine (Leu), casein
Acid (Tyr), phenylalanine (Phe), β- alanine (β-Ala), β- aminoisobutyric acid (β-Aia), γ- aminobutyric acid (γ-Aba), lysine acid
(Lys), histidine (His), tryptophan (Trp), arginine (Arg) and other 21 kinds of Determination of free amino acids.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Preparation and water YC/T 31-1996 Tobacco and tobacco products - Determination of the sample oven method
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Tobacco extract hydrochloric acid in the presence of a free state, unbound amino acids in the protein molecule.
Principle 4
Amino acid ampholytes form cations in an acidic environment. Tobacco free amino acids after acid solution was extracted, by amino acid
Analyzer sulfonate type lithium-ion exchange column, and then mixed with ninhydrin, by heating the reaction, with a primary amine compound generated blue-violet, sec
With an amine to form a yellow compound. Two Derivatives with a wavelength of 570nm and 440nm dual channel UV detector were carried out at the same time
Qualitative and Quantitative Determination (amino acid analyzer line diagram see Appendix A).
5 Reagents
In addition to special requirements, this standard reagents used were of analytical reagent water is deionized water.
5.1 hydrochloric acid solution, 0.005mol/L.
Accurate Pipette 420μL of concentrated hydrochloric acid (mass fraction of 36% -38%) in 1000mL volumetric flask with water to volume. This solution
It was used as the extraction solution.
5.2 sample dilution, pH2.20.
Accurately weighed four water lithium citrate 11.3g, citric acid, 6.0g, was dissolved in 500mL water and then adding 20mL25% (mass fraction
Number) thiodiglycol solution and 15mL32% (mass fraction) of hydrochloric acid solution. Transferred to a 1000mL volumetric flask, make to volume with water.
Lithium-ion 5.3 buffer solution 1)
1) preparation of a lithium-ion buffer solution is recommended, the operator can adjust according to the situation of the use of the instrument.
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