Search result: SN/T 3084.1-2012
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Occular irritation test of cosmetics for import and export. Neutral red uptake assays in vitro
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SN/T 3084.1-2012
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| Standard ID | SN/T 3084.1-2012 (SN/T3084.1-2012) | | Description (Translated English) | Occular irritation test of cosmetics for import and export. Neutral red uptake assays in vitro | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 10,115 | | Quoted Standard | GB/T 6682; SN/T 2285 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China | | Summary | This standard specifies the use of neutral red uptake assay in vitro eye irritation potential of cosmetic method of operation. This standard applies to soluble surfactants and surfactant-based cosmetic raw materials and formulations. This standard applies |
SN/T 3084.1-2012
Occular irritation test of cosmetics for import and export.Neutral red uptake assays in vitro
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Importers cosmetic eye irritation test
In vitro neutral red absorption method
Issued on. 2012-05-07
2012-11-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This section drafted in accordance with GB/T 1.1-2009 given rules.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Guangdong Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this section. Shixi Ju, Ma Guiping, Zeng Jing, Cheng Shujun, Li Bingling, Liyan Xin, Liu Quanguo, Liu Xuhui.
Importers cosmetic eye irritation test
In vitro neutral red absorption method
1 Scope
This section specifies the method of operation used in vitro neutral red absorption assay cosmetic eye irritation potential.
This section applies to soluble surfactants and surfactant-based cosmetic ingredients and formulations.
This part applies to weak eye irritation test products for poorly soluble, volatile, non-ferrous and lysosomal reactive substances does not apply.
This section is a pre-screening test cosmetics eye irritation potential, it can be used as in vitro eye irritation test method or combination of methods a series
Points, cosmetic eye irritation final classification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 2285 cosmetic vitro alternatives experiments
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this document.
3.1.1 Cell viability
Metric cell population total activity, to express to absorbance at 540nm (OD540nm).
3.1.2 relative cell viability
Participants to the vitality and viability of the negative control cells ratio of cell material, in this relative light absorbance at 540nm
(OD540nm%) represented.
3.2 Acronyms
The following abbreviations apply to this document.
DMEM. cell culture medium.
NRU. neutral red.
DMSO. dimethyl sulfoxide.
PBS. phosphate buffer (cell wash liquor).
SDS. sodium dodecyl sulfate.
IC50. make BALB/c cells neutral red adsorption capacity reduction of 50% concentration is the test substance.
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