SN/T 4877.10-2017 English PDF

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SN/T 4877.10-2017: DNA barcoding screening method - Part 10: quarantine Phytophthora
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Similar standards

GBZ 57   GB/T 31989   SN/T 4877.14   SN/T 4877.15   SN/T 4877.13   

Basic data

Standard ID: SN/T 4877.10-2017 (SN/T4877.10-2017)
Description (Translated English): DNA barcoding screening method - Part 10: quarantine Phytophthora
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C62
Word Count Estimation: 19,156
Date of Issue: 2017-08-29
Date of Implementation: 2018-04-01
Regulation (derived from): National Quality Inspection (2017) 384
Issuing agency(ies): General Administration of Customs

SN/T 4877.10-2017: DNA barcoding screening method - Part 10: quarantine Phytophthora

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Screening methods for gene barcodes - Part 10. Phytophthora) People's Republic of China entry and exit inspection and quarantine industry standards Gene barcode screening method Part 10. Quarantine Phytophthora DNAbarcodingscreeningmethod-Part 10. QuarantinePhytophthora Published on.2017-08-29 2018-04-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 4877 "Gene Barcode Screening Method" is divided into 10 parts. --- Part 1. Quarantine coryneform bacteria; --- Part 2. Phytophthora xanthan; --- Part 3. Quarantine phytoplasma; --- Part 4. Quarantine stem mold; --- Part 5. Quarantine Pseudomonas spp.; --- Part 6. Quarantine acidophilus; --- Part 7. Phytophthora Verticillium; --- Part 8. Quarantine anthrax; --- Part 9. Quarantine smut; --- Part 10. Quarantine Phytophthora. This part is the 10th part of SN/T 4877. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine. Drafters of this section. Zhang Guiming, Gao Ruifang, Wang Ying, Cheng Yinghui, Wang Ying, Huang Heqing. Gene barcode screening method Part 10. Quarantine Phytophthora

1 Scope

This part of SN/T 4877 specifies Phytophthora capsici blackwater pathogen, Phytophthora capsici rot, Phytophthora infestans, Phytophthora raspberry Root rot, Phytophthora lucidum, Phytophthora capsici root rot, Phytophthora root rot, Phytophthora sojae, eucalyptus, and soybean disease DNA barcode screening of mold and Phytophthora syriae. This section applies to Phytophthora capsici blackwater pathogen, Phytophthora capsici, Phytophthora erythraea, Phytophthora capsici, root rot, citrus Phytophthora sojae, Phytophthora capsici root rot, Phytophthora root rot, Phytophthora sojae, eucalyptus, Phytophthora sojae and clove Amplification, analysis and determination of results of DNA barcode screening of Phytophthora infestans.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 1131 Phytophthora Phytophthora Quarantine and Identification Method SN/T 1135.6 Quarantine and identification method for potato rot SN/T 2080 Quarantine and identification method for eucalyptus death Real-time fluorescent PCR detection method for SN/T 2474 Phytophthora sojae SN/T 2617 Phytophthora Phytophthora Quarantine Identification Method SN/T 2756 Phytophthora Phytophthora Quarantine Identification Method SN/T 2759 Quarantine and identification method for Phytophthora capsici SN/T 3403 Phytophthora Phytophthora Quarantine Identification Method

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 DNA barcode DNAbarcode A standard, sufficiently variable, easily amplified and relatively short DNA fragment of the species that can represent the species. 3.2 Mitochondrial cytochrome c oxidase subunit I gene mitochondrialcytochromecoxidasesubunitI, COI The rate of mitochondrial gene changes rapidly. Among the 13 protein-coding genes, there are few insertions and deletions in the COI gene, and the sequence length is 500 bp~ 700bp, suitable as a fungal DNA barcode, is also the earliest proposed DNA barcode fragment. 3.3 Internal transcribed spacer sequence internaltranscribedspacer; ITS In eukaryotes, ribosomal DNA consists of a ribosome gene and a spacer adjacent to it, with a genome sequence from 5' to 3' In order. external transcribed spacer, 18S gene, internal transcribed spacer 1 (ITS1), 5.8S gene, internal transcribed spacer 2 (ITS2), 28S gene and gene spacer sequence. The internal transcribed spacer is the region present between 18SrDNA, 5.8SrDNA and 28SrDNA ......

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