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SN/T 4853.3-2017 English PDF

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SN/T 4853.3-2017: Quantitative detection of genetically modified rice. Digital PCR - Part 3: Event KF6
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 4853.3-2017209 Add to Cart 3 days Quantitative detection of genetically modified rice. Digital PCR - Part 3: Event KF6 Valid

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Basic data

Standard ID: SN/T 4853.3-2017 (SN/T4853.3-2017)
Description (Translated English): Quantitative detection of genetically modified rice. Digital PCR - Part 3: Event KF6
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X11
Word Count Estimation: 9,919
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Quoted Standard: GB/T 6682; GB/T 19495.1; GB/T 19195.2; GB/T 19495.3; GB/T 19495.5; GB/T 19495.7; SN/T 1194
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 337
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the digital PCR quantitative detection method for the rice and rice Zhongkefeng 6 strains. This standard applies to the digital PCR quantitative detection of rice and rice in the Kefeng No. 6 strain. The method has a quantitative detection limit (LOQ) of 0.1% (mass fraction).

SN/T 4853.3-2017: Quantitative detection of genetically modified rice. Digital PCR - Part 3: Event KF6


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quantitative detection of genetically modified rice.Digital PCR - Part 3. Event KF6 People's Republic of China entry and exit inspection and quarantine industry standards GM rice quantitative detection digital PCR Part 3. Kefeng No. 6 strain Part 3. EventKF6 Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 4853 "GMO quantitative detection digital PCR method" series of standards are divided into 7 parts. --- Part 1. TT51-1 strain; --- Part 2. Kezhen rice line; --- Part 3. Kefeng No. 6 strain; --- Part 4. M12 strain; --- Part 5. LL62 strain; --- Part 6. T2A-1 strain; --- Part 7. T1C-19 strain. This part is the third part of the SN/T 4853 standard. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. China Academy of Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people And the National Jilin Entry-Exit Inspection and Quarantine Bureau. The main drafters of this section. Huang Wensheng, Chen Ying, Deng Tingting, Wu Yajun, Fu Wei, Nie Dandan, Zhu Shuifang, Han Jianxun, Li Xiang. GM rice quantitative detection digital PCR Part 3. Kefeng No. 6 strain

1 Scope

This section specifies the digital PCR quantitative detection method for the rice and rice Zhongkefeng 6 strains. This section applies to the digital PCR quantitative detection of rice and rice in the Kefeng No. 6 strain. The method has a quantitative detection limit (LOQ) of 0.1% (mass fraction).

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 19495.1 General requirements and definitions for the detection of genetically modified products GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products GB/T 19495.3 Transgenic product detection nucleic acid extraction and purification method GB/T 19495.5 Genetically modified nucleic acid quantitative PCR detection method for genetically modified products GB/T 19495.7 GM product testing sampling and sample preparation method SN/T 1194 Detection and sampling method for genetically modified components of plants and their products 3 terms and definitions, abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 SPS gene Thegeneofsucrosephosphatesynthase Sucrose phosphate synthase gene, rice single copy internal reference gene. 3.1.2 Kefeng 6 transformant specific sequence event-specificsequenceofKF6 The contiguous region sequence resulting from the recombination of the foreign DNA into the recipient rice genome. See Appendix A for specific sequences. 3.1.3 Taq enzyme TaqDNApolymerase A thermostable DNA polymerase derived from Azotobacter. 3.1.4 Template template Specific DNA fragments detected by digital PCR primer/probe amplification.
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