SN/T 4827-2017 English PDF

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SN/T 4827-2017: Quarantine protocol for Chryseobacterium meningosepticum of frog
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4827-2017	279	Add to Cart	3 days	Quarantine protocol for Chryseobacterium meningosepticum of frog	Valid

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Basic data

Standard ID: SN/T 4827-2017 (SN/T4827-2017)
Description (Translated English): Quarantine protocol for Chryseobacterium meningosepticum of frog
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 12,188
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 337
Issuing agency(ies): General Administration of Customs

SN/T 4827-2017: Quarantine protocol for Chryseobacterium meningosepticum of frog

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical specification for quinatal brucellosis in frog meningitis) People's Republic of China entry and exit inspection and quarantine industry standards Technical specification for quarantine of frog meningitis Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Jiangsu Province Entry and Exit Inspection Epidemic situation. The main drafters of this standard. Xu Shufei, Kong Fande, Xie Mingxing, Tang Taishan, Wang Kaimin, Jiang Yan. Technical specification for quarantine of frog meningitis

1 Scope

This standard specifies the clinical symptoms and bacteriology, PCR and other laboratory diagnosis and detection methods for frog meningitis. This standard is applicable to the epidemiological investigation, monitoring, diagnosis and entry-exit inspection and quarantine of frog meningitis.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 18088 entry and exit animal quarantine sampling

3 reagents and media

Unless otherwise specified, the following biochemical reagents were of analytical grade. See Appendix A for the reagent preparation method in the text. 3.1 Nutritional broth. 3.2 Tryptone soy agar (TSA). 3.3 thiosulfate citrate bile salt sucrose agar (TCBS). 3.4 Nutritional agar. 3.5 Biochemical identification kit. 3.6 Gram staining solution. 3.7 DNA extraction reagents. related reagents required for proteinase K, SDS, phenol-chloroform extraction; commercial DNA extraction kit. 3.8 PCR reagent. 10× concentrated buffer, dNTP, magnesium chloride, 6× electrophoresis loading buffer, agarose, 5× running buffer (TBE) Etc., all of the ingredients in the commercial kit. 3.9 Primers. a) Specific primers. ---P1.5'-GATTCGTCGGATTATATTG-3'; ---P2. 5'-CCACTTCAACCTTACTCAAGACTAAC-3'. The concentration was 10 μmol/L, and the size of the amplified product was 475 bp to 479 bp. b) Universal primers. ---P3. 5'-AGAGTTTGATCMTGGCTCAG-3'; ---P4. 5'-GGYTACCTTGTTACGACTT-3'. The concentration was 10 μmol/L, and the size of the amplified product was 1488 bp. 3.10 Negative and positive controls. provided by the designated laboratory of the AQSIQ. 3.11 DNADL2000Marker. It is suitable for detecting DNA fragments between 100bp and.2000bp in molecular weight and stored at -20°C.

4 Disease Overview

Frog meningitis, septicemia, caused by meningitis, Elizabethkingiameningoseptica ......

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