SN/T 4821-2017 English PDFUS$209.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 4821-2017: Detection method for bovine complex vertebral malformation by TaqMan PCR Status: Valid
Basic dataStandard ID: SN/T 4821-2017 (SN/T4821-2017)Description (Translated English): Detection method for bovine complex vertebral malformation by TaqMan PCR Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Word Count Estimation: 9,979 Date of Issue: 2017-07-21 Date of Implementation: 2018-03-01 Quoted Standard: GB/T 6682 Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 337 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the detection method of TaqMan probe PCR for bovine spinal deformity syndrome (see Appendix A). This standard is applicable to rapid screening of carriers of recessive bovine spondylolisthesis genes in cattle. SN/T 4821-2017: Detection method for bovine complex vertebral malformation by TaqMan PCR---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection method for bovine complex vertebral malformation by TaqMan PCR People's Republic of China entry and exit inspection and quarantine industry standards Bovine spinal deformity syndrome TaqMan probe PCR Detection method Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. Please note that some of the contents of this document may involve patents. The issuing authority of this document shall not be responsible for identifying these patents. This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the General Administration of Quality Supervision, Inspection and Quarantine, and the Chinese People Xiamen Entry-Exit Inspection and Quarantine Bureau of the Republic. The main drafters of this standard. Wu Xiaowei, Liu Zhongyong, Jia Guangle, Peng Xiaoli, Chen Ru, Zhu Daozhong, Yu Haiqiong, Liu Zhiling, Duan Yanyu, Lin Zhixiong. Bovine spinal deformity syndrome TaqMan probe PCR Detection method1 ScopeThis standard specifies the detection method for TaqMan probe PCR in bovine spinal deformity syndrome (see Appendix A). This standard is applicable to rapid screening of carriers of recessive bovine spondylolisthesis genes in cattle.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods3 AbbreviationsThe following abbreviations apply to this document. CVM Spinal Malformation Syndrome (ComplexVertebralMalformation) TaqMan Probe TaqMan Probe (TaqManprobes) PCR polymerase chain reaction (Polymerasechainreaction) Cycle number (Cyclethreshold) experienced when the Ct value fluorescence signal reaches the set threshold SN P single nucleotide polymorphism (Singlenucleotide polymorphism)4 PrincipleThe principle of the SN P typing detection technology of the TaqMan method. when the ordinary polymerase chain reaction is based, the pair of two ends are different. Fluorescently labeled specific probes to recognize different alleles (alele1 and alele2). The 5' end is a reporter fluorophore (reporter), The 3' end is a quenching fluorophore (quencher). Complementary sequence-specific annealing junction between the two probes and the forward and reverse primers Hehe. When the probe is present in its intact form, the fluorophore emits only weak fluorescence due to energy resonance transfer; when the specific probe is associated with After the alleles are heterozygous, the DNA polymerase exerts 5' to 3' exonuclease activity, and the fluorescent reporter group is cleaved and detached from the 3' end. The quenching of the photo group thereby emits a fluorescent signal. According to the difference of the detected fluorescent signal, the corresponding sample can be judged SN P allele type.5 instruments and reagent supplies5.1 Instruments and equipment Fluorescence quantitative PCR instrument. Constant temperature water bath. 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