SN/T 4822-2017 English PDF

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SN/T 4822-2017: Detection method of deficiency of uridine monophosphate synthase by RT-PCR
Status: Valid

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Basic data

Standard ID: SN/T 4822-2017 (SN/T4822-2017)
Description (Translated English): Detection method of deficiency of uridine monophosphate synthase by RT-PCR
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 7,788
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 337
Issuing agency(ies): General Administration of Customs

SN/T 4822-2017: Detection method of deficiency of uridine monophosphate synthase by RT-PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Detection method of bovine uridine synthase deficiency by fluorescence quantitative) People's Republic of China entry and exit inspection and quarantine industry standards Bovine uridine synthase deficiency Fluorescent quantitative PCR detection method Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Li Hongquan, Sun Liangjuan, Zhang Na, Liu Zhongyong, Xie Yanhui, Wu Xiaowei, Li Jiaqiao, Zhou Guangxuan, Zhu Shikang, Zhu Daozhong, Ma Baohua, Liu Zhiling, Lin Zhixiong, Yu Haiqiong. Bovine uridine synthase deficiency Fluorescent quantitative PCR detection method

1 Scope

This standard specifies the method for the detection of bovine uridine synthase deficiency by quantitative PCR. This standard applies to the monitoring, diagnosis and identification of bovine uridine synthase deficiency (see Appendix A).

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this document. Ct value Cycle number (cyclethreshold) experienced when the fluorescence signal in each reaction tube reaches a set threshold DUMPS uridine acid synthase deficiency (deficiency ofuridine monophosphatesynthase) RT-PCR real-time fluorescence quantitative PCR (realtimefluorescence quantitative PCR) SN P single nucleotide polymorphism (singlenucleotide polymorphism)

4 reagents and materials

4.1 Phenol (C6H6O). 4.2 Trichloromethane (CHCl3). 4.3 Isoamyl alcohol [(CH3)2CHCH2CH2OH]. 4.4 Phenol-trichloromethane-isoamyl alcohol. saturated re-steamed phenol. chloroform.isoamyl alcohol was mixed in a ratio of 25.24.1. 4.5 Protease K Lysate (10mg/mL). Dissolve 100mg of Proteinase K in 10mL of Sterilized Distilled Water and Store at -20°C Stand by and avoid repeated freezing and thawing. 4.6 SDS solution. Dissolve.200g of electrophoresis grade SDS in 900mL water, heat to 68 °C to help dissolve, add concentrated hydrochloric acid to adjust the pH value to 7.2, plus The water was made up to 1000 mL and stored at room temperature. 4.7 DNA extraction and purification kit. 4.8 Real-time PCR Master Kit (Realtime PCR MasterMix), available from Reagents or other equivalent kits. 4.9 Sterilize ddH2O. 4.10 Primers and probes (concentration is 10 μmol/L). Fl. 5'-ttctgaatttgtgattggttttat-3' Rl. 5'-cctcctgcttctaactgaactc-3' probe-W. 5'-VIC-ctggctcCgagtaagca-BHQ-3' probe-M. 5'-FAM-ctggctcTgagtaagca-BHQ-3' ......

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