SN/T 4747.2-2017 English PDF

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SN/T 4747.2-2017: Determination of macrolide residues in live animal for import and export. MIA method
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SN/T 4747.2-2017	189	Add to Cart	3 days	Determination of macrolide residues in live animal for import and export. MIA method	Valid

Similar standards

GB/T 9959.1   SB/T 10381   SB/T 10294   SN/T 4747.3   SN/T 4744   SN/T 4747.1   

Basic data

Standard ID: SN/T 4747.2-2017 (SN/T4747.2-2017)
Description (Translated English): Determination of macrolide residues in live animal for import and export. MIA method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X22
Word Count Estimation: 8,875
Date of Issue: 2017-05-12
Date of Implementation: 2017-12-01
Regulation (derived from): National Quality Inspection (2017) 228
Issuing agency(ies): General Administration of Customs

SN/T 4747.2-2017: Determination of macrolide residues in live animal for import and export. MIA method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Method for determination of macrolide residues in edible animals) People's Republic of China entry and exit inspection and quarantine industry standards Import and export of animal macrolides Determination of microbial inhibition export-MIAmethod Released on.2017-05-12 2017-12-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This part is the second part of the SN/T 4747 series of standards. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Wang Naifu, Huang Chen, Wu Dongxue, Wang Wanqi, Liu Yang, Dong Zhizhen, Zhao Xiangping. Import and export of animal macrolides Determination of microbial inhibition

1 Scope

This part of SN/T 4747 specifies the preparation of samples and microbial inhibition of the detection of residues of macrolides and veterinary drugs in imported and exported food animals. Detection method. This part is applicable to the screening and testing of macrolide lactone residues in blood and urine of imported and exported food animals during entry-exit inspection and quarantine. Positive results need to be confirmed by other methods.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and experimental methods

3 method summary

After the sample is allowed to stand or centrifuge, the supernatant is added to a medium containing a certain amount of M. luteus, and the antibiotic is used for the sensitive bacteria. Inhibition to determine whether the sample contains antibiotics.

4 reagents and materials

4.1 Micrococcus luteus CMCC28001. 4.2 Preservation and Subculture Media. See Appendix AA.1. 4.3 Enrichment solid medium. see A.2. 4.4 assay medium. see A.3. 4.5 Methanol. 4.6 Phosphate buffer. Weigh 0.523g potassium dihydrogen phosphate (KH2PO4) and 16.73g dipotassium hydrogen phosphate (K2HPO4) with distilled water Dissolve and dilute to 1000 mL, autoclave at 121 ° C for 15 min. 4.7 Standard substance erythromycin. purity ≥ 98%. 4.8 Erythromycin standard stock solution. 10μg/mL. Weigh 20.0mg of the standard substance erythromycin (accurate to 0.1mg), first dissolve with a small amount of methanol Solution, then make up to.2000mL with phosphate buffer, store in 2 ° C ~ 8 ° C refrigerator, can be used 7d. 4.9 erythromycin standard working solution. take erythromycin standard stock solution, diluted with phosphate buffer to a concentration of 0.05μg/mL, 0.1μg/mL, Standard working solutions of 0.2 μg/mL, 0.4 μg/mL, and 0.8 μg/mL must be prepared for use on the same day.

5 instruments and equipment

5.1 Petri dish. 90mm in diameter, flat and smooth at the bottom, with ceramic tile cover. 5.2 Centrifuge. The minimum centrifugal force is not less than 4000g. ......

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