SN/T 4747.1-2017 English PDFUS$149.00 · In stock
Delivery: <= 2 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 4747.1-2017: Determination of macrolide residues in live animal for import and export. Radio-receptor assay method Status: Valid
Basic dataStandard ID: SN/T 4747.1-2017 (SN/T4747.1-2017)Description (Translated English): Determination of macrolide residues in live animal for import and export. Radio-receptor assay method Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X22 Word Count Estimation: 5,559 Date of Issue: 2017-05-12 Date of Implementation: 2017-12-01 Regulation (derived from): State-Quality-Inspection-Accreditation (2017) 228 Issuing agency(ies): General Administration of Customs SN/T 4747.1-2017: Determination of macrolide residues in live animal for import and export. Radio-receptor assay method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. (Method for determination of macrolide residues in edible animals) People's Republic of China entry and exit inspection and quarantine industry standards Import and export of animal macrolides Radiation receptor assay Released on.2017-05-12 2017-12-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis part is the first part of the SN/T 4747 series of standards. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part is proposed and managed by the National Certification and Accreditation Supervision Committee. This section drafted by. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Zhang Lihuai, Chen Benlong, Chai Mingjun, Yang Chao, Wang Wanqi, Liu Yang, Huang Chen. Import and export of animal macrolides Radiation receptor assay1 ScopeThis part of SN/T 4747 specifies the detection method for macrolide residues in the blood and urine of imported and exported food animals. This section applies to erythromycin, tylosin, spiramycin, lincomycin and other macrolides in import and export food activities. Micardo's drug residue screening test.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6882 analytical laboratory water specifications and test methods3 Method OverviewRadioreceptor analysis is based on the binding of drug functional groups to microbial receptor sites, which share functions with certain classes of antibiotics. Group-related, this specificity of the application of the receptor allows for multi-residue analysis of a certain class of antibiotics. In this method, the macro ring remaining in the sample After the lactone drug is extracted and diluted, it competes with the [14C]-labeled erythromycin to bind to a specific receptor site, and after reacting at a certain temperature, Centrifugation to remove unbound macrolides, and finally adding scintillation fluid to determine [14C] decay of beta-particle radioactivity cpm (countperminute). The measured cpm value is inversely related to the amount of macrolide antibiotic residues in the sample.4 reagents and materials4.1 Macrolide antibiotic (Charm-II Assay 1) (MRLSequential Assay) 1). 1) This information is given for the convenience of the users of this section and does not imply endorsement of the operation steps of a particular product. If other products have steps Different, must be used after experimental evaluation. 4.2 Negative control sample. macrolide antibiotic negative control concentrated dry powder (MNC), use 10mL water to dissolve, shake well, so that Warm back to room temperature before use. Long-term storage should be between 2 ° C and 6 ° C. 4.3 MSU multi-antibiotic concentration standard (MSUMA, which is a commercialized macrolide residue Residue Charm-II assay kit Band). A standard containing a variety of macrolide antibiotics. Dissolve with 10mL of water when using, shake well, use in the refrigerator or ice before use Place in the bath for 15 min. The reconstituted standard contained 10000 μg/kg erythromycin. 4.4 MSU Extraction Buffer Concentrated dry powder. Dissolve in 1000 mL of water, shake well, and warm to room temperature before use. Available at 2 ° C~ Store at 6 ° C for 2 months. 4.5 M2 buffer. Take concentrated concentrated powder in 50mL water when used, prepare M2 buffer, adjust the pH value of serum sample, Store at 2 ° C ~ 6 ° C for 2 months. 4.6 0.1mol/L NaOH, prepared by yourself, adjust the pH value of the urine sample. 4.7 0.1mol/L HCl, prepared by yourself, adjust the pH value of the urine sample. 4.8 Scintillation fluid. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 4747.1-2017_English be delivered?Answer: Upon your order, we will start to translate SN/T 4747.1-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 2 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 4747.1-2017_English with my colleagues?Answer: Yes. 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