SN/T 4728-2016 English PDF

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SN/T 4728-2016: Detection and identification of Coconut tinangaja viroid
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SN/T 4728-2016	329	Add to Cart	3 days	Detection and identification of Coconut tinangaja viroid	Valid

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Basic data

Standard ID: SN/T 4728-2016 (SN/T4728-2016)
Description (Translated English): Detection and identification of Coconut tinangaja viroid
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,143
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4728-2016: Detection and identification of Coconut tinangaja viroid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Identification method of coconut virus) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Quarantine identification method for coconut aboriginal virus Released on December 12,.2016 2017-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Hainan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. Drafters of this standard. Li Weidong, Pan English, Liu Fuxiu, Han Yuchun, Yu Su. Quarantine identification method for coconut aboriginal virus

1 Scope

This standard specifies the quarantine and identification methods for coconut-borne viruses. This standard applies to the quarantine and identification of coconut-borne viruses in palm plants, seedlings and plants.

2 Normative references

The following documents are indispensable for the reference to this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products 3 Basic information about coconut-borne viruses Scientific name. Coconuttinangajaviroid Abbreviation. CTiVd Chinese name. Coconut aboriginal virus Taxonomic status. Pospiviroidae, a potato spindle tuber viroid, and Cocadviroid, a coconut death virus. See Appendix A for additional information on coconut-borne viruses.

4 Principle of the method

The genome of a coconut-borne virus is circular or linear, and the nucleic acid is a single-stranded ribonucleic acid (ssRNA). Planting based on this type of virus The strain specificity, the genomic RNA sequence, and the like are specifically detected by PCR, and the results are determined by the electrophoresis band size or the Ct value.

5 instruments and main reagents

5.1 Instrumentation PCR instrument, real-time PCR instrument, ultra-clean workbench, sterilizer, ice machine, nucleic acid protein analyzer, high-speed refrigerated centrifuge, table Small centrifuges, ultra-low temperature refrigerators, conventional refrigerators, vortex shakers, micro-injectors, electrophoresis systems, gel imaging systems, electronic analytical balances The amount is 0.1 mg) and the like. 5.2 main reagent All reagents were of analytical grade unless otherwise stated. RT-PCR detection reagent (see Appendix B), fluorescent quantitative PCR detection reagent (see Appendix C).

6 Sampling and sample preparation

Observe the symptoms of plant damage at the quarantine site. For the description of the symptoms of CTiVd, see A.3. For plants suspected of having viral symptoms, each strain ......

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