SN/T 4538.4-2016 English PDF

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SN/T 4538.4-2016: Commercial kit method--Melamine--Test method Ⅳ
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4538.4-2016	139	Add to Cart	2 days	Commercial kit method--Melamine--Test method Ⅳ	Valid

Similar standards

GB/T 687   GB/T 693   SN/T 4538.2   SN/T 4538.3   SN/T 4538.1   

Basic data

Standard ID: SN/T 4538.4-2016 (SN/T4538.4-2016)
Description (Translated English): Commercial kit method--Melamine--Test method ��
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: G66
Word Count Estimation: 6,626
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4538.4-2016: Commercial kit method--Melamine--Test method Ⅳ

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Commercial kit detection method melamine method four) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Commercial kit detection method Melamine method four Published on.2016-08-23 2017-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 4538 "Commercial kit detection method melamine" is divided into the following four parts. ---SN/T 4538.1 Commercialization kit detection method melamine method one; ---SN/T 4538.2 Commercialization kit detection method melamine method 2; ---SN/T 4538.3 Commercialization kit detection method melamine method three; ---SN/T 4538.4 Commercialization kit detection method melamine method IV. This part is the fourth part of the SN/T 4538 series of standards. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. China Academy of Inspection and Quarantine, Beijing Qinbang Biotechnology Co., Ltd. The main drafters of this section. Zou Mingqiang, Wang Yanfei, Xue Qiang, Liu Caihong, Ma Jixiang. Commercial kit detection method Melamine method four

1 Scope

This part of SN/T 4538 specifies the method for the determination of melamine immunocolloidal gold in dairy products. This section applies to the screening of melamine in milk and milk powder by immunocolloidal gold method.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this standard. SN/T 2775 Commercialized Food Testing Kit Evaluation Method

3 Principle

Applying the principle of immunocompetitive chromatography, the melamine in the sample flows with the colloidal gold-labeled specific monoclonal antibody Binding, reducing or preventing the binding of the colloidal gold-labeled antibody to the upper melamine-BSA conjugate of the NC membrane detection line, making the detection line shallow It even disappeared to qualitatively detect melamine in the sample.

4 reagents and materials

4.1 Melamine rapid test strip card It is transported and stored at 2 °C ~ 8 °C, and the product is valid for more than 12 months. The kit reference SN/T 2775 was evaluated and evaluated by the National Accreditation Administration Committee for the Evaluation of Commercialized Food Testing Kits. See Appendix A for the results. The kit consists of the following. a) Test strip. coated with melamine-specific antibody labeled with colloidal gold, labeled with melamine conjugate on the test line; b) microporous reagents; c) Instruction manual.

5 Instruments and equipment

Pipette.200 μL.

6 sample preparation

6.1 Liquid milk sample The sample to be tested needs to be returned to room temperature and mixed thoroughly. ......

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