SN/T 4537.2-2016 English PDF

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SN/T 4537.2-2016: Commercial kit method--Chloramphenicol--Test method Ⅱ
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4537.2-2016	229	Add to Cart	3 days	Commercial kit method--Chloramphenicol--Test method Ⅱ	Valid

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GB 5009.271   GB 5009.22   SN/T 2641   SN/T 4544.3   SN/T 4537.3   SN/T 4537.1   

Basic data

Standard ID: SN/T 4537.2-2016 (SN/T4537.2-2016)
Description (Translated English): Commercial kit method--Chloramphenicol--Test method ��
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X04
Word Count Estimation: 10,154
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Older Standard (superseded by this standard): SN/T 2057-2008
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 4537.2-2016: Commercial kit method--Chloramphenicol--Test method Ⅱ

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Commercialization kit detection method chloramphenicol method II) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Replace SN/T 2058-2008 Commercial kit detection method Chloramphenicol Method Two Released on December 12,.2016 2017-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 4537 "Commercial kit detection method chloramphenicol" is divided into three parts. ---SN/T 4537.1. Method 1; ---SN/T 4537.2. Method 2; ---SN/T 4537.3. Method three. This part is the second part of SN/T 4537. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part replaces SN/T 2058-2008 "Method for determination of chloramphenicol residues in import and export royal jelly" by enzyme-linked immunosorbent assay, Compared with SN/T 2058-2008, the main technical changes are as follows. --- Part of the name from the "Import and export royal jelly in the determination of chloramphenicol residues in the enzyme-linked immunoassay" was changed to "Commodity kit check Method for measuring chloramphenicol method II. --- Add the evaluation results of the kit to the appendix. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Zhejiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Netherlands EURO-PROXIMA company. The main drafters of this section. Zhang Xiaofeng, Wu Hao, Shao Honghong, Shuai Jiangbing, Li Ke. The previous versions of the standards replaced by this section are. ---SN/T 2058-2008. Commercial kit detection method Chloramphenicol Method Two

1 Scope

This part of SN/T 4537 specifies an enzyme-linked immunosorbent assay for the determination of chloramphenicol residues in royal jelly and lyophilized powder. This section applies to the screening and determination of chloramphenicol residues in royal jelly and lyophilized powder.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 2775 Commercialized Food Testing Kit Evaluation Method

3 Method summary

This standard uses trichloroacetic acid to precipitate the protein of royal jelly and extract chloramphenicol from royal jelly, and then purify it with a solid phase extraction column. Microplate Medium-coated sheep anti-rabbit IgG antibody, specific antibody (rabbit anti-chloramphenicol antibody), enzyme labeled chloramphenicol, chloramphenicol standard or sample After the extract, the specific antibody binds to the coated sheep anti-rabbit IgG antibody, and the free chloramphenicol and the enzyme labeled chloramphenicol compete with each other. Specific antibody binding. Unbound chloramphenicol and enzyme-labeled chloramphenicol were removed by washing, then the substrate was added for color development, and the absorbance was measured by a microplate reader. The luminosity is based on the absorbance and the chloramphenicol content in the sample.

4 reagents and materials

All reagents were of analytical grade except water, and the water was the first grade water specified in GB/T 6682. 4.1 Chloramphenicol test kit. This kit was evaluated by the Expert Committee of the National Certification and Quarantine Committee for the Evaluation of Commercialized Foods in accordance with SN/T 2775. Specific evaluation See Appendix A. The kit consists of the following. a) 96-well plates pre-coated with antibodies. 12 x 8 wells. b) Chloramphenicol standard solutions. 0.025 ng/mL, 0.05 ng/mL, 0.1 ng/mL, 0.2 ng/mL, 0.5 ng/mL, 2 ng/mL and 100 ng/mL. c) Recombinant/zero standard buffer. d) Chloramphenicolase label lyophilized powder. chloramphenicol can be formulated with recombinant/zero standard buffer according to the instructions in the chloramphenicol kit Marker solution. e) Anti-chloramphenicol antibody lyophilized powder. According to the instructions in the chloramphenicol kit, it can be formulated with anti-chloramphenicol antibiotics using recombinant/zero standard buffer. Body solution. f) Substrate TMB solution. ......

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