SN/T 4537.1-2016 English PDF

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SN/T 4537.1-2016: Commercial kit method--Chloramphenicol--Test method Ⅰ
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4537.1-2016	139	Add to Cart	2 days	Commercial kit method--Chloramphenicol--Test method Ⅰ	Valid

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GB 5009.271   GB 5009.22   SN/T 2641   SN/T 4544.3   SN/T 4537.3   SN/T 4537.2   

Basic data

Standard ID: SN/T 4537.1-2016 (SN/T4537.1-2016)
Description (Translated English): Commercial kit method--Chloramphenicol--Test method ��
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X04
Word Count Estimation: 6,618
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Older Standard (superseded by this standard): SN/T 2058-2008
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4537.1-2016: Commercial kit method--Chloramphenicol--Test method Ⅰ

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Commercial kit detection method Chloramphenicol Method One) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Commercial kit detection method Chloramphenicol method one Published on.2016-08-23 2017-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued Entry and exit inspection and quarantine Industry Standard Commercial kit detection method Chloramphenicol method one China Standard Press Publishing First edition in December.2017

Foreword

This part is the first part of the SN/T 4537 series of standards. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. China Academy of Inspection and Quarantine, Beijing Qinbang Biotechnology Co., Ltd. The main drafters of this section. Zou Mingqiang, Xue Qiang, Wang Yanfei, Wan Yuping, Luo Xiaoqin. Commercial kit detection method Chloramphenicol method one

1 Scope

This part of SN/T 4537 specifies the method for chloramphenicol enzyme-linked immunosorbent assay in food. This section applies to the quantitative determination of chloramphenicol in chicken and shrimp by enzyme-linked immunoassay.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 2775 Commercialized Food Testing Kit Evaluation Method

3 Principle

The antigen is pre-coated on the microplate of the microplate, and the chloramphenicol and the pre-coated conjugated antigen on the microporous strip compete with the chloramphenicol The enzyme conjugate is developed with TMB substrate, and the absorbance of the sample is negatively correlated with the content of the residue chloramphenicol. The chloramphenicol content of the sample can be obtained by the corresponding dilution factor.

4 reagents and materials

4.1 Chloramphenicol enzyme-linked immunosorbent assay kit It is transported and stored at 2 °C ~ 8 °C. The product is valid for 12 months. The kit reference SN/T 2775 was evaluated and evaluated by the National Accreditation Administration Committee for the Evaluation of Commercialized Food Testing Kits. See Appendix A for the results. The kit consists of the following. a) 96-well microtiter plate × 1 block, coated with coupled antigen; b) Standard solution × 6 bottles, 1 mL/bottle, the concentration is 0 × 10-9, 0.025 × 10-9, 0.075 × 10-9, 0.3 × 10-9, 1.2 × 10-9, 4.8×10-9; c) high concentration standard. 1mL/bottle, the concentration is 100 × 10-9; d) enzyme conjugate working solution, 7mL; e) substrate liquid A, 7mL; f) substrate liquid B, 7mL; g) Stop solution, 7mL; h) 20× concentrated washing solution, 40 mL; i) 2 x concentrated complex solution, 50 mL. 4.2 ethyl acetate (analytical grade) 4.3 n-hexane (analytical grade) 4.4 Deionized water ......

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